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General Information
Symbol
Dmel\PGRP-LE112
Species
D. melanogaster
Name
FlyBase ID
FBal0182386
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
LE112
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{GS}PGRP-LEGS1068 insertion has created a 2542bp deletion which includes the PGRP-LE start codon.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

PGRP-LE112 adults infected with Erwinia carotovora carotovora by oral feeding exhibit an increase in the immune response (immune deficiency pathway activation) in proventriculus compared to controls.

PGRP-LE112 homozygous individuals exhibit a significantly increased bacterial load after infection with the non-pathogenic E. coli, as compared to equally infected controls.

Mutant flies show reduced survival after infection with Erwinia carotovora carotovora by septic injury compared to controls.

PGRP-LE112 flies show lower survival rates after L. monocytogenes infection compared to controls.

Hemolymph clots from PGRP-LE112 third instar larvae show normal melanization.

The survival rate of mutant animals after bacterial infections is similar to that of wild-type except for infection by P.carotovorum.carotovorum, and Gram-positive bacterial infections, B.megaterium, B.subtilis, E.faecalis and M.luteus. After infection with E.coli weak melanisation is induced at the injury site in mutant animals.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

PGRP-LE112 has abnormal immune response | conditional phenotype, non-suppressible by PGRP-LBΔ

Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

PGRP-LE112 is a non-suppressor of visible | adult stage phenotype of PGRP-LF200

Other
Phenotype Manifest In
NOT suppressed by
Statement
Reference

PGRP-LE112 has adult proventriculus | conditional phenotype, non-suppressible by PGRP-LBΔ

Suppressor of
Statement
Reference

PGRP-LE112 is a suppressor | partially of adult midgut | conditional phenotype of PGRP-LBΔ

NOT Suppressor of
Statement
Reference

PGRP-LE112 is a non-suppressor of adult proventriculus | conditional phenotype of PGRP-LBΔ

PGRP-LE112 is a non-suppressor of adult fat body | conditional phenotype of PGRP-LBΔ

PGRP-LE112 is a non-suppressor of wing phenotype of PGRP-LF200

Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of PGRP-LCΔE and PGRP-LE112 leads to an absence of immune deficiency pathway activation in proventriculus of adults infected with Erwinia carotovora carotovora by oral feeding different than controls.

PGRP-LE112, PGRP-LCΔE double homozygotes show a significant increase in viral load in the head upon infection with ZIKV, as compared to infected double heterozygotes.

The reduced survival rate upon systemic infection with Escherichia coli observed in PGRP-LCΔE mutants is substantially decreased further in PGRP-LE112;PGRP-LCΔE double mutants.

PGRP-LCΔE, PGRP-LE112 double mutants do not exhibit any difference in survival rate of flies either untreated or treated with Leishmania amastigotes, as compared to wild type.

The reduced survival of PGRP-LBΔ flies after infection with Erwinia carotovora carotovora is suppressed if they are also mutant for PGRP-LE112.

Expression of ListericinScer\UAS.cGa using Scer\GAL4Cg.PA restores the survival phenotype against L. monocytogenes infection in a PGRP-LE112 background.

PGRP-LE112;PGRP-LCΔE double mutant flies are rapidly killed by infection with the gram-negative pathogen Erwinia carotovora carotovora.

PGRP-LE112 does not suppress the notching phenotypes and low adult viability seen in homozygous PGRP-LF200 mutants.

Unlike single mutants, PGRP-LC7454/PGRP-LE112 double mutant animals exhibit a reduced resistance to E.coli and B.megaterium infection, but not E.faecalis and M.luteus infection.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (27)