FlyBase Allele Report: Dmel\PGRP-LE[112]

General Information

Symbol

Dmel\PGRP-LE¹¹²

Species

D. melanogaster

Name

FlyBase ID

FBal0182386

Feature type

allele

Associated gene

Dmel\PGRP-LE

Associated Insertion(s)

Carried in Construct

Also Known As

LE¹¹²

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Takehana et al., 2004)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

PGRP-LE^GS1068

(Takehana et al., 2004)

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of the P{GS}PGRP-LE^GS1068 insertion has created a 2542bp deletion which includes the PGRP-LE start codon.

(Takehana et al., 2004)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response

(Neyen et al., 2012, Goto et al., 2010, Takehana et al., 2004)

abnormal immune response | adult stage | recessive

(Wong et al., 2017)

abnormal immune response | conditional

(Charroux et al., 2018)

fertile

(Takehana et al., 2004)

viable

(Takehana et al., 2004)

Phenotype Manifest In

adult proventriculus | conditional

(Charroux et al., 2018)

Detailed Description

Statement

Reference

PGRP-LE¹¹² adults infected with Erwinia carotovora carotovora by oral feeding exhibit an increase in the immune response (immune deficiency pathway activation) in proventriculus compared to controls.

(Charroux et al., 2018)

PGRP-LE¹¹² homozygous individuals exhibit a significantly increased bacterial load after infection with the non-pathogenic E. coli, as compared to equally infected controls.

(Wong et al., 2017)

Mutant flies show reduced survival after infection with Erwinia carotovora carotovora by septic injury compared to controls.

(Neyen et al., 2012)

PGRP-LE¹¹² flies show lower survival rates after L. monocytogenes infection compared to controls.

(Goto et al., 2010)

Hemolymph clots from PGRP-LE¹¹² third instar larvae show normal melanization.

(Bidla et al., 2007)

The survival rate of mutant animals after bacterial infections is similar to that of wild-type except for infection by P.carotovorum.carotovorum, and Gram-positive bacterial infections, B.megaterium, B.subtilis, E.faecalis and M.luteus. After infection with E.coli weak melanisation is induced at the injury site in mutant animals.

(Takehana et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

PGRP-LE¹¹² has abnormal immune response phenotype, enhanceable by PGRP-LC⁷⁴⁵⁴

(Takehana et al., 2004)

Suppressed by

Statement

Reference

PGRP-LE¹¹² has abnormal immune response phenotype, suppressible by Listericin^UAS.cGa/Scer\GAL4^Cg.PA

(Goto et al., 2010)

NOT suppressed by

Statement

Reference

PGRP-LE¹¹² has abnormal immune response | conditional phenotype, non-suppressible by PGRP-LB^Δ

(Charroux et al., 2018)

Enhancer of

Statement

Reference

PGRP-LE¹¹² is an enhancer of abnormal immune response | adult stage phenotype of PGRP-LC^ΔE/PGRP-LC^ΔE

(Paik et al., 2017)

PGRP-LE¹¹² is an enhancer of abnormal immune response phenotype of PGRP-LC⁷⁴⁵⁴

(Takehana et al., 2004)

Suppressor of

Statement

Reference

PGRP-LE¹¹² is a suppressor | partially of abnormal immune response | conditional phenotype of PGRP-LB^Δ

(Charroux et al., 2018)

PGRP-LE¹¹² is a suppressor of abnormal immune response phenotype of PGRP-LB^Δ

(Bosco-Drayon et al., 2012)

PGRP-LE¹¹² is a suppressor of partially lethal - majority die phenotype of PGRP-LF²⁰⁰

(Maillet et al., 2008)

NOT Suppressor of

Statement

Reference

PGRP-LE¹¹² is a non-suppressor of visible | adult stage phenotype of PGRP-LF²⁰⁰

(Maillet et al., 2008)

Other

Statement

Reference

PGRP-LC^ΔE, PGRP-LE¹¹² has abnormal immune response | recessive | adult stage phenotype

(Liu et al., 2018)

PGRP-LC^ΔE, PGRP-LE¹¹² has abnormal immune response phenotype

(Aggarwal et al., 2008)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

PGRP-LE¹¹² has adult proventriculus | conditional phenotype, non-suppressible by PGRP-LB^Δ

(Charroux et al., 2018)

Suppressor of

Statement

Reference

PGRP-LE¹¹² is a suppressor | partially of adult midgut | conditional phenotype of PGRP-LB^Δ

(Charroux et al., 2018)

NOT Suppressor of

Statement

Reference

PGRP-LE¹¹² is a non-suppressor of adult proventriculus | conditional phenotype of PGRP-LB^Δ

(Charroux et al., 2018)

PGRP-LE¹¹² is a non-suppressor of adult fat body | conditional phenotype of PGRP-LB^Δ

(Charroux et al., 2018)

PGRP-LE¹¹² is a non-suppressor of wing phenotype of PGRP-LF²⁰⁰

(Maillet et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of PGRP-LC^ΔE and PGRP-LE¹¹² leads to an absence of immune deficiency pathway activation in proventriculus of adults infected with Erwinia carotovora carotovora by oral feeding different than controls.

(Charroux et al., 2018)

PGRP-LE¹¹², PGRP-LC^ΔE double homozygotes show a significant increase in viral load in the head upon infection with ZIKV, as compared to infected double heterozygotes.

(Liu et al., 2018)

The reduced survival rate upon systemic infection with Escherichia coli observed in PGRP-LC^ΔE mutants is substantially decreased further in PGRP-LE¹¹²;PGRP-LC^ΔE double mutants.

(Paik et al., 2017)

PGRP-LC^ΔE, PGRP-LE¹¹² double mutants do not exhibit any difference in survival rate of flies either untreated or treated with Leishmania amastigotes, as compared to wild type.

(Okuda et al., 2016)

The reduced survival of PGRP-LB^Δ flies after infection with Erwinia carotovora carotovora is suppressed if they are also mutant for PGRP-LE¹¹².

(Bosco-Drayon et al., 2012)

Expression of Listericin^Scer\UAS.cGa using Scer\GAL4^Cg.PA restores the survival phenotype against L. monocytogenes infection in a PGRP-LE¹¹² background.

(Goto et al., 2010)

PGRP-LE¹¹²;PGRP-LC^ΔE double mutant flies are rapidly killed by infection with the gram-negative pathogen Erwinia carotovora carotovora.

(Aggarwal et al., 2008)

PGRP-LE¹¹² does not suppress the notching phenotypes and low adult viability seen in homozygous PGRP-LF²⁰⁰ mutants.

(Maillet et al., 2008)

Unlike single mutants, PGRP-LC⁷⁴⁵⁴/PGRP-LE¹¹² double mutant animals exhibit a reduced resistance to E.coli and B.megaterium infection, but not E.faecalis and M.luteus infection.

(Takehana et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PGRP-LE¹¹² is rescued by PGRP-LE^UAS.Tag:FLAG/Scer\GAL4^He.PZ

(Wong et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33055

y¹ w^67c23 PGRP-LE¹¹²

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

LE¹¹²

(Nagai et al., 2021, Goto et al., 2010)

pgrp le¹¹²

(Okuda et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (27)

Report Sections

Open Close