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General Information
D. melanogaster
FlyBase ID
Feature type
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dumb2, DopRf02676, f02676, dumb2
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
Additional Notes
Associated Sequence Data
DNA sequence
Protein sequence
Progenitor genotype
Nature of the lesion
Insertion components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Modifiers Based on Experimental Evidence ( 0 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
Disease-implicated variant(s)
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description

Dop1R1f02676 homozygous mutant adults do not have an increase in aversive olfactory 24 h memory observed when the recall environment better matches the training environment (presence of copper grid) unlike controls.

Dop1R1f02676 heterozygous larvae have a shift in odor-induced increases in mouth hook contractions towards higher concentrations of odorants, compared to controls.

Dop1R1f02676 homozygous adults show an apparently normal sensitivity to ethanol as compared to controls: they shown an apparently normal time until sedation and a normal tolerance development following daily exposures; they also show normal ethanol-induced disinhibition, as shown by the increasing frequency of inter-male courtship upon continual exposure.

Dop1R1f02676 mutant adults have significantly reduced 24-hour associative olfactory water-reward memory, compared to controls, with no alteration in odor acuity or water preference.

Dop1R1f02676 mutant flies do not show increased resistance to paraquat intoxication compared to controls.

Dop1R1[f02676] adults have significantly impaired long-term, but not short-term, appetitive olfactory memory, compared to controls.

Dop1R1f02676 mutants exhibit significantly impaired water learning.

In contrast to wild type controls, rapamycin does not affect night activity, night sleep, night sleep bout number or bout duration in Dop1R1f02676 mutants.

Dop1R1f02676 mutant adults have significantly reduced aversive and appetitive 1-min visual memory, compared to controls and significantly altered naive electric shock, but not sugar preference, compared to controls.

Homozygous flies show a severe defect in long-term olfactory memory measured 24 hours after spaced repetitive training.

Expression of DopRScer\UAS.cKa under the control of Scer\GAL4ey-OK107 in a homozygous DopRf02676 background (which will also result in the expression of DopR from the Scer\UAS regulatory sequences present in the insertion in DopRf02676) results in a severe defect in long-term olfactory memory measured 24 hours after spaced repetitive training.

DopRf02676 mutant flies show a similar increase in sugar sensitivity in response to L-Dopa feeding to wild controls.

The decreased night-time sleep seen when wild-type flies are fed L-dopa is markedly suppressed in a DopRf02676/DopRf02676 background.

Dop1R1f02676 mutant adults have significantly reduced associative appetitive olfactory memory, compared to controls.

DopRf02676 mutants show reduced ethanol-induced hyperactivity and no change in ethanol sedation sensitivity or ethanol absorption. DopRf02676 also show increased activity prior to ethanol exposure.

DopRf02676/Df(3R)ED5634 flies show reduced ethanol-induced hyperactivity.

DopRf02676 mutants perform indistinguishably from wild-type in a rapid tolerance paradigm, showing both increased ethanol-induced hyperactivity and increased resistance to the sedating effects of ethanol during a second exposure to ethanol vapor.

Dop1R1f02676 mutant larvae show a significantly reduced performance in aversive and appetitive olfactory learning (using amylacetate (AM) and benzaldehyde (BA)) compared to controls. Perception of AM and BA, salt and sugar are all similar to wild type.

DopRf02676/DopRf02676 and DopRf02676/In(3LR)234 adults fail to learn to avoid specific odors after conditioning by concurrent shock and odor exposure. The same results are seen when testing is done immediately after conditioning or 1 hour after conditioning and with a variety of odors. These animals also have significantly reduced scores in tests for sugar reward mediated olfactory conditioning.

External Data
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions

Dop1R1f02676 does not suppress the activity and sleep defects seen in Df(3L)Ilp2-3, Ilp51 mutant flies.

DopRf02676/+ suppresses the defect in naive odour avoidance response to 4-methylcyclohexanol (1 x 10[-3] v/v) which is seen in mir-276aRosa/mir-276aD8 flies.

The reduction in nighttime sleep seen in flies expressing TrpA1Scer\UAS.(B).cKa under the control of Scer\GAL4ple.PF at 27[o]C is fully suppressed by Dop1R1f02676/Dop1R1f02676.

Xenogenetic Interactions
Complementation and Rescue Data

Expression of Dop1R1Scer\UAS.cLa in mostly the γ (with Scer\GAL4NP1131 or Scer\GAL4Tab2-201Y), but not the αβ (Scer\GAL4c739) or α'β' (Scer\GAL4c305a) mushroom body neurons, produces a significant restoration of memory performance.

Expressing DopRf02676 in Gr5a gustatory receptor neurons under the control of Scer\GAL4Gr5a.8.5 rescues the defects in the sugar-sensitive proboscis extension reflex (PER) seen in DopRf02676 mutant females.

Expression of DopR (from the Scer\UAS sequences in the PBac{WH}DopRf02676 insertion) under the control of either Scer\GAL4104Y or Scer\GAL4c205 significantly restores the the reduced sleep caused by dopamine signaling in DopRf02676/DopRf02676 flies fed L-dopa. This effect is not seen if expression is driven either by Scer\GAL4ey-OK107 or by Scer\GAL4c547.

Flies of the genotype Scer\GAL45.30/+; DopRf02676/DopRf02676 show selectively restored expression of DopR. Those flies that had restored expression in patterns that include the R2/R4 class ellipsoid body neurons exhibit rescue of ethanol-induced hyperactivity. Restoring DopR expression in the R3 neurons of the ellipsoid body neurons (through Scer\GAL4lilli-189Y) results in a modest but statistically significant increase in ethanol-induced hyperactivity. However, expression with Scer\GAL4Aph-4-c232 has no effect on ethanol induced hyperactivity in DopRf02676 homozygous mutants.

The failure of DopRf02676/In(3LR)234 or DopRf02676/DopRf02676 adults to learn to avoid specific odors after conditioning by concurrent shock and odor exposure is partially rescued by Scer\GAL4elav-C155 and completely rescued by Scer\GAL4Mef2.247. Scer\GAL4elav-C155 and Scer\GAL4Mef2.247 also completely rescued learning by sugar-reward conditioning in these flies. (In all cases, rescue was tested in the presence of Scer\GAL80ts.αTub84B and rescued was seen at 30'C but not at room temperature.)

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Synonyms and Secondary IDs (8)
References (34)