mesothoracic ventral neurosecretory neuron & axon, with Scer\GAL4FMRFa.PS
metathoracic ventral thoracic neurosecretory neuron & axon, with Scer\GAL4FMRFa.PS
prothoracic ventral neurosecretory neuron & axon, with Scer\GAL4FMRFa.PS
Axons of motorneuron clones of a laterally located lineage (identified as lineage 15) expressing EcRA-ΔC655.W650A.Scer\UAS generally show missing arbors in the adult femur and tibia, misaligned and clumped neuromuscular junctions, and about half the number of femoral endplates of controls. Seven out of fifteen clones were found to have clumped or misaligned proximal femoral branches, showing a star-shaped morphology projecting away from the primary tract. In approximately half the clones examined, the distal femoral arbors are completely missing, but of those still present many are misaligned and the remaining show a clumped phenotype.
Thoracic ventral neurosecretory cells that express EcRB1-ΔC655.W650A.Scer\UAS under the control of Scer\GAL4Fmrf.PS are of normal size and larval morphology at the start of metamorphosis. However, these mutants show defective neuronal remodelling during metamorphosis. The axons show much lower levels of pruning compared to wild-type cells and show no filopodia during the pruning phase. Effects on adult axon outgrowth are severe, with arbors significantly reduced in size.