W28term | Dronc-PA
G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.
Nearly all vCrz neurons are present at 7 hours after puparium formation in dream4; NcI24 double mutants. Such an abnormal programmed cell death phenotype is significantly more severe than in either single mutant. No surviving vCrz neurons are found at 7 hours after puparium formation in dream4; NcI24/+ mutants, suggesting that heterozygosity of Nc is sufficient to compensate for the lack of dream function.
Triple mutants of DreddB118; dream4; NcI24 display results comparable to dream4; NcI24 double mutants, i.e. all vCrz neurons are still present having not undergone apoptosis by 7 hours after puparium formation.
Females containing dream4 ; NcI24 double homozygous germline clones are fertile, but their ovaries contain 31% of mid-stage egg chambers with missing follicle cells and large surviving nurse cells. There is also an increase in mid-stage egg chambers containing condensed nurse cell nuclei but lacking follicle cells. 16% of stage 14 egg chambers contain persisting nurse cell nuclei.