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General Information
Symbol
Dmel\DroncI24
Species
D. melanogaster
Name
FlyBase ID
FBal0190335
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G9968970A

Amino acid change:

W28term | Dronc-PA

Reported amino acid change:

W28term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: ?28term.

Amino acid replacement: W28term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

NcI24 mutants exhibit an increase in autophagy.

NcI24/NcI29 mutant males exhibit ~40% less spermatogonial cyst death compared to controls.

NcI24/NcL32 mutant males exhibit ~40% less spermatogonial cyst death compared to controls.

NcI24 mutant eye clones are significantly larger than their wild-type twin spots.

NcI24 mutant eye clones induce ectopic cell proliferation posterior to the second mitotic wave in the eye-antennal disc.

NcI24 mutant eye clones exhibit extra ommatidial pigment cells in the pupal retina.

NcI24 homozygotes and NcI24/Nc51 transheterozygotes exhibit a delay in vCrz neuron apoptosis. At 7 hours after puparium formation 75% vCrz neurons are still present although these all die by 48 hours after puparium formation.

NcI24 mutant dendrites exhibit an intact morphlogy before puparium formation. Unlike in wild-type, at 7.5 hours after puparium formation NcI24 mutant dendrites continue to possess an overall regular morphology and are mostly attached to the parental neurons. These branches persist even at 18 hours after puparium formation, albeit with less homogenous morphology.

Homozygous adult survivors have approximately 6-8 extra branches on the posterior of the arista compared to wild type. Approximately 10% of stage 14 egg chambers contain persisting nurse cell nuclei.

Salivary glands are degraded by 24 hours after puparium formation (as occurs in wild-type controls) in NcI24/NcI29 mutants that develop normally.

100% of NcI29/NcI24 male escapers have a rotated genitalia defect. 100% of NcI29/NcI24 adult escapers have a ballooned wing phenotype.

NcI24 mutants display severe defects during the spermatid individualization process. The cystic bulges are frequently reduced in size or appear flat due to a failure in the appropriate collection of the cytoplasm of the spermatids . The retained cytoplasm is clearly visualized as a trail along the entire length of what was supposed to have been the post-individualized portion of the spermatids. Frequently a large portion of the spermatid cytoplasm is retained in a 'mini' cystic bulge structure, which often contains part of the individualization complex. Waste bags are also reduced in size.

Programmed cell death in Crz-expressing neurons in the ventral nerve cord is significantly delayed in Nc51/NcI24 mutants. Approximately 12 neurons still survive at 7 hours after pupal formation, while the number is reduced to approximately 8 at 16 hours after pupal formation, and none at 48 hours after pupal formation.

Homozygous escapers have wings that are less transparent than normal and are curved. Homozygous embryos derived from females carrying homozygous germline clones have a head defect. These embryos show a substantial reduction in the number of apoptotic cells compared to wild type. Homozygous clones in the pupal eye disc have on average three additional interommatidial cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

DroncI24, Strica4 has abnormal cell death phenotype, non-enhanceable by DreddB118

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

DroncI24, Strica4 has abnormal cell death phenotype, non-suppressible by DreddB118

Enhancer of
Statement
Reference

DroncI24 is an enhancer of abnormal cell death phenotype of Strica4

Suppressor of
Statement
Reference
NOT Suppressor of
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

DroncI24 has neuron phenotype, enhanceable by Strica4

NOT Enhanced by
Statement
Reference

DroncI24, Strica4 has neuron phenotype, non-enhanceable by DreddB118

Suppressed by
Statement
Reference

DroncI24/Dronc51 has neuron phenotype, suppressible by DreddL23

NOT suppressed by
Statement
Reference

DroncI24, Strica4 has neuron phenotype, non-suppressible by DreddB118

Enhancer of
Suppressor of
Statement
Reference

DroncI24/Dronc[+] is a suppressor of eye phenotype of Srrm1GE25979, Scer\GAL4GMR.PU

DroncI24/DroncL32 is a suppressor | partially of ovariole phenotype of Diap16B/Diap18

DroncI24/DroncI24 is a suppressor of eye | somatic clone phenotype of rprGMR.PW

NOT Suppressor of
Statement
Reference

DroncI24/Nc[+] is a non-suppressor of eye | somatic clone phenotype of hidGMR.PG/WGMR.PG

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

One copy of DroncI24 slightly suppresses the eye roughness seen when SRm160GE25979 is expressed under the control of Scer\GAL4GMR.PU.

No viable vCrz neurons are detected at 7 hours after puparium formation in DreddL23; NcI24/Nc51 double mutants.

Nearly all vCrz neurons are present at 7 hours after puparium formation in dream4; NcI24 double mutants. Such an abnormal programmed cell death phenotype is significantly more severe than in either single mutant. No surviving vCrz neurons are found at 7 hours after puparium formation in dream4; NcI24/+ mutants, suggesting that heterozygosity of Nc is sufficient to compensate for the lack of dream function.

Triple mutants of DreddB118; dream4; NcI24 display results comparable to dream4; NcI24 double mutants, i.e. all vCrz neurons are still present having not undergone apoptosis by 7 hours after puparium formation.

Dcp-1Prev1 ; NcI24/NcI29 IceΔ1 triple mutant larvae undergo cell death with morphology similar to the midgut of wild-type animals when analysed from -4 to -1 hours relative to puparium formation.

dream4 ; NcI24/NcI29 IceΔ1 triple mutant larvae show high levels of TUNEL staining at -4 to -1 hours relative to puparium formation, suggesting that they are undergoing cell death.

Females containing dream4 ; NcI24 double homozygous germline clones are fertile, but their ovaries contain 31% of mid-stage egg chambers with missing follicle cells and large surviving nurse cells. There is also an increase in mid-stage egg chambers containing condensed nurse cell nuclei but lacking follicle cells. 16% of stage 14 egg chambers contain persisting nurse cell nuclei.

The persistence of salivary gland tissue that is seen at 24 hours hours after puparium formation when Pi3K92EScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4fkh.PH is significantly enhanced if the animals are also carrying NcI24/NcI29.

Xenogenetic Interactions
Statement
Reference

The adult wing defects caused by expression of HIV-1\VpuScer\UAS.cLa under the control of Scer\GAL4dpp.blk1 are partially suppressed by the presence of NcI24/+.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: a recessive suppressor of the WGMR.PG eye ablation phenotype.

Selected as: a mutant that recessively suppresses the eye ablation phenotype caused by eye-specific overexpression of W.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (23)