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General Information
Symbol
Dmel\Gyc76C2388
Species
D. melanogaster
Name
FlyBase ID
FBal0190851
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

T to G change at the 5' end of the intron following exon 16, changing the conserved GU at the splice donor site into GG. This results in the insertion of 92bp of sequence from intron 6, which contains an in-frame stop codon before the guanylyl cyclase domain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In Gyc76C2388 heterozygous embryos, all salivary gland cells invaginate to form a gland that turns posteriorly during stages 13 and 14. By contrast, in Gyc76C2388 homozygous embryos, 16% of mutant glands do not invaginate completely, with some cells remaining at the ventral surface. Gyc76C2388 homozygous embryos display a severe salivary gland migration defect where 88% of mutant glands fail to turn completely and some glands appear branched or folded.

In Gyc76C2388 heterozygous embryos, the salivary gland lumen is a single continuous structure formed during the process of invagination. By contrast, in Gyc76C2388 homozygous embryos, the salivary gland lumen is branched with ectopic lumens along the length of the central lumen. Branching of the salivary gland lumen in Gyc76C2388 mutant embryos occurs through indentation of the apical membrane during invagination or through formation of an ectopic invagination site.

Gyc76CKG03723ex173/Gyc76C2388 heterozygous embryos display a severe salivary gland migration defect where mutant gland fail to turn completely and some glands appear branched or folded.

Homozygous embryos show defects in the somatic muscle pattern. Numerous ventral and lateral muscle fail to extend or are missing. Free, unfused fusion competent myoblasts are present in some of the mutant embryos. Dorsal muscle patterning is largely unaffected. 60% of homozygous embryos show muscle defects at stage 15, but this is reduced to 20% at stage 16, indicating delayed muscle development.

Patterning of the ventral and lateral somatic muscles is abnormal in Gyc76CKG03723ex173/Gyc76C2388 embryos, whereas dorsal muscles are patterned normally.

Patterning of the ventral and lateral somatic muscles is abnormal in Gyc76CKG03723ex173/Df(3L)XS533 embryos.

In gim2388 embryos, the tracheal branches fail to migrate in a wild-type pattern; the dorsal trunk and visceral branches are the most severely affected.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

82% of salivary glands LanB2MB04039 Gyc76C2388 transheterozygous embryos do not migrate completely.

LanB2MB04039 Gyc76C2388 double mutants exacerbate the salivary gland invagination defect such that 25% of mutant glands fail to invaginate compared to 16% in Gyc76C2388 and 13% in LanB2MB04039 single mutant glands.

Gyc76C2388/Df(3R)e-19 transheterozygotes do not show defects in salivary gland development.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Gyc76CScer\UAS.T:Hsap\MYC in either the salivary glands or mesoderm, under the control of Scer\GAL4fkh.PH or Scer\GAL4twi.PB significantly rescues the gland migration defect found in Gyc76C2388 homozygous embryos. Simultaneous expression of Gyc76CScer\UAS.T:Hsap\MYC in the salivary gland and mesoderm does not result in increased rescue of the migration defect compared to expression in either tissue alone. In contrast to the rescue of the migration defects, the salivary gland invagination defects of Gyc76C2388 mutant embryos are better rescued by expression of Gyc76CScer\UAS.T:Hsap\MYC in the gland than in the mesoderm.

Expression of Gyc76CScer\UAS.T:Hsap\MYC under the simultaneous control of both Scer\GAL4Mef2.PR and Scer\GAL4twi.PB partially rescues the somatic muscle defects of Gyc76C2388 homozygous embryos, such that only 10% of stage 14 and 5% of stage 16 embryos show a patterning defect.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: a mutation that disrupts tracheal development when homozygous.

The mutant "gim[2388]" chromosome originally contained two separable lethal mutations: one at 76B-77B (corresponding to a mutation in Gyc76C) which results in salivary gland defects, and one at 93B-93D (uncovered by Df(3R)e-19) which does not result in in salivary gland defects.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
gim2388
gyc76C2388
Name Synonyms
Secondary FlyBase IDs
    References (3)