T to G change at the 5' end of the intron following exon 16, changing the conserved GU at the splice donor site into GG. This results in the insertion of 92bp of sequence from intron 6, which contains an in-frame stop codon before the guanylyl cyclase domain.
LanB2MB04039 Gyc76C2388 double mutants exacerbate the salivary gland invagination defect such that 25% of mutant glands fail to invaginate compared to 16% in Gyc76C2388 and 13% in LanB2MB04039 single mutant glands.
Expression of Gyc76CScer\UAS.T:Hsap\MYC in either the salivary glands or mesoderm, under the control of Scer\GAL4fkh.PH or Scer\GAL4twi.PB significantly rescues the gland migration defect found in Gyc76C2388 homozygous embryos. Simultaneous expression of Gyc76CScer\UAS.T:Hsap\MYC in the salivary gland and mesoderm does not result in increased rescue of the migration defect compared to expression in either tissue alone. In contrast to the rescue of the migration defects, the salivary gland invagination defects of Gyc76C2388 mutant embryos are better rescued by expression of Gyc76CScer\UAS.T:Hsap\MYC in the gland than in the mesoderm.
Selected as: a mutation that disrupts tracheal development when homozygous.