FlyBase Allele Report: Dmel\Drice[Δ1]

General Information

Symbol

Dmel\Drice^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0193685

Feature type

allele

Associated gene

Dmel\Drice

Associated Insertion(s)

P{?EP}Drice^Δ1

Carried in Construct

Also Known As

Ice^Δ1

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Muro et al., 2006)

Mutagen

P-element activity

(Muro et al., 2006)

Progenitor genotype

P{EP}Drice^GE28489

(Muro et al., 2006)

Associated Insertion(s)

P{?EP}Drice^Δ1

Cytology

Description

Imprecise excision of the P{EP} element, resulting in deletion of the entire Ice coding region and fusion of the Ice 5' and 3' UTR sequences. A 1.5kb fragment of the P{EP} element remains.

(Muro et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates retinitis pigmentosa

modeled by ninaE^P37H, ninaE¹⁷

(Griciuc et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death

(Lee et al., 2011)

abnormal cell death | drug conditional

(Muro et al., 2006)

abnormal cell death | somatic clone

(Link et al., 2007)

abnormal developmental rate | partially | pupal stage

(Muro et al., 2006)

abnormal neuroanatomy | adult stage

(Lee et al., 2013)

abnormal neuroanatomy | adult stage (with Drice¹⁷)

(Lee et al., 2013)

decreased cell death

(Florentin and Arama, 2012, Muro et al., 2006)

decreased cell death | adult stage

(Lee et al., 2013)

decreased cell death | adult stage (with Drice¹⁷)

(Lee et al., 2013)

increased cell death

(Yacobi-Sharon et al., 2013)

increased cell death | P-stage | recessive

(Akagawa et al., 2015)

lethal - all die before end of pupal stage

(Muro et al., 2006)

male fertile

(Muro et al., 2006)

neoplasia | pupal stage

(Muro et al., 2006)

partially lethal - majority die

(Lee et al., 2013, Florentin and Arama, 2012)

radiation resistant

(Muro et al., 2006)

short lived

(Lee et al., 2013)

some die during larval stage

(Florentin and Arama, 2012)

some die during pupal stage

(Muro et al., 2006)

Phenotype Manifest In

adult abdomen

(Muro et al., 2006)

adult optic lobe | P-stage

(Akagawa et al., 2015)

arista

(Muro et al., 2006)

dendrite (with Drice¹⁷), with Scer\GAL4^ppk.PG

(Schoenmann et al., 2010)

male genitalia

(Muro et al., 2006)

male germline cell

(Yacobi-Sharon et al., 2013)

midline glial cell

(Muro et al., 2006)

neuron

(Lee et al., 2011)

neuron (with Drice¹⁷), with Scer\GAL4^ppk.PG

(Schoenmann et al., 2010)

peptidergic neuron | adult stage | ectopic

(Lee et al., 2013)

peptidergic neuron | adult stage | ectopic (with Drice¹⁷)

(Lee et al., 2013)

retina

(Muro et al., 2006)

spermatid cyst

(Muro et al., 2006)

spermatogonial cyst

(Yacobi-Sharon et al., 2013)

wing

(Muro et al., 2006)

wing | somatic clone

(Link et al., 2007)

wing disc

(Florentin and Arama, 2012)

Detailed Description

Statement

Reference

In Drice^Δ1 mutants, the peak number of apoptotic cells in the optic lobe is delayed to 36h APF and the number of apoptotic cells is increased relative to wild type. In addition, the spatial distribution of apoptotic cells throughout different regions of the optic lobe is significantly different compared to wild type. Cell corpse clearance is decreased throughout the optic lobe in regions of cell death, as compared to wild type.

(Akagawa et al., 2015)

The abdominal ganglion where axonal bundles exit to innervate abdominal tissue is substantially elongated and broader in homozygous Ice^Δ1 mutants. The programmed cell death of bursCCAP neurons seen in the adult ventral nerve cord of wild type flies after eclosion is strongly suppressed; six bursCCAP neurons survive in 3-5 day old Ice^Δ1 mutant flies.

The programmed cell death of bursCCAP neurons seen in the adult ventral nerve cord of wild type flies after eclosion is strongly suppressed in Ice^Δ1/Ice¹⁷ mutants; six bursCCAP neurons survive in 3-5 day old flies.

(Lee et al., 2013)

Levels of germ cell death are slightly increased in Ice^Δ1 mutant spermatogonial cysts.

(Yacobi-Sharon et al., 2013)

Ice^Δ1 mutant wing discs display a complete block in cell death, even after 50 Gy irradiation.

Ice^Δ1 mutant larva exhibit a <20% survival rate to adulthood.

(Florentin and Arama, 2012)

All 16 vCrz neurons are present at 7 hours after puparium formation in Ice^Δ1 mutants. The surviving neurons display budding structures similar to those of dying neurons in wild-type flies at 1-2 hours after puparium formation. The number of vCrz neurons is progressively reduced to 13 at 12-16 hours after puparium formation and 7 at 24 hours after puparium formation.

(Lee et al., 2011)

Ice¹⁷/Ice^Δ1 trans-heterozygous mutants exhibit a reduction in dendritic branch pruning at 18 hours after puparium formation.

(Schoenmann et al., 2010)

Ice^Δ1 homozygous adult 'escapers' present normal wings at eclosion but develop blemishes with age.

(Link et al., 2007)

80% of Ice^Δ1 mutants die following puparium formation. A similar survival rate is observed for Ice^Δ1 mutants derived from homozygous Ice^Δ1 mothers.

Most (73%) of homozygous Ice^Δ1 pupae appear similar to controls after puparium formation. The remaining 27% are arrested in development. Of these arrested pupae, one-third fail to undergo head eversion and arrest prior to the commencement of salivary gland cell death. The other two thirds appear to have arrested 1 or 2 hours after head eversion, and thus the salivary glands and larval muscle are not degraded, and the larval gut has failed to condense properly. All Ice^Δ1 pupae possess many abnormal masses in the head, abdomen, wing disc and leg disc. The frequency of these masses are increased by almost two-fold when Ice^Δ1 animals are subjected to X-irradiation during larval stages.

The arista of Ice^Δ1 flies has a much thicker central shaft and many more lateral branches than the arista of wild-type flies. The genitalia of approximately 50% of Ice^Δ1 male adults is markedly rotated compared to wild-type males. Additionally, more than 50% of Ice^Δ1 adults have an open 'scar' of varying severity along the dorsal abdominal midline. The tissue appears thin and fragile, with some flies possessing holes or tears in this area associated with leakage of hemolymph.

Ice^Δ1 flies have opaque wings, which sometimes contain trapped fluid or are not fully extended. Visualization of cell death in Ice^Δ1 wings shows that cells persist in the posterior compartment of the wing that have died in the posterior compartment of wild-type wings.

The retinae of Ice^Δ1 flies show on average three additional interommatidial cells per ommatidia compared to wild-type retinae in which cell death eliminates excess interommatidial cells.

Ice^Δ1 embryos show lower levels of cell death than wild-type embryos, according to TUNEL labeling. In addition, stage 17 Ice^Δ1 embryos contain on average 6 midline glia per segment while wild-type embryos contain only 3 midline glia per segment.

The wing discs of Ice^Δ1 flies do not show increased cell death response to irradiation that wild-type discs show. Ice^Δ1 hemocytes do not die in response to cycloheximide as occurs in wild-type cells.

The spermatid cysts of Ice^Δ1 testis show a partial failure in individualization.

(Muro et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Drice^Δ1 has lethal | pupal stage phenotype, enhanceable by Dcp-1[+]/Dcp-1^Prev1

(Muro et al., 2006)

Drice^Δ1 has abnormal developmental rate | pupal stage phenotype, enhanceable by Dcp-1^Prev1

(Muro et al., 2006)

NOT suppressed by

Statement

Reference

Drice^Δ1 has decreased cell death phenotype, non-suppressible by Dcp-1^Prev1

(Florentin and Arama, 2012)

Drice^Δ1 has partially lethal - majority die | larval stage phenotype, non-suppressible by Dcp-1^Prev1

(Florentin and Arama, 2012)

Suppressor of

Statement

Reference

Drice^Δ1/Drice¹⁷ is a suppressor of visible phenotype of hid^GMR.PG

(Wu et al., 2016)

Drice^Δ1/Drice¹⁷ is a suppressor of visible phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Drice^L1 is a suppressor of visible phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Drice^L2 is a suppressor of visible phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^C1/Drice^Δ1 is a suppressor of visible phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Drice^C2 is a suppressor of visible phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Drice^S1 is a suppressor of visible phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Drice^S2 is a suppressor of visible phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Ice[+] is a suppressor of abnormal neurophysiology phenotype of ninaE¹⁷, ninaE^P37H

(Griciuc et al., 2014)

Drice^Δ1/Ice[+] is a suppressor of abnormal neuroanatomy phenotype of ninaE¹⁷, ninaE^P37H

(Griciuc et al., 2014)

Drice^Δ1, Dcp-1[+], Dcp-1^Prev1 is a suppressor of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Drice^Δ1/Ice[+] is a suppressor of viable | conditional phenotype of S2P^KG08356/S2P¹

(Amarneh et al., 2009)

Drice^Δ1/Drice^Δ1 is a suppressor of viable | conditional phenotype of S2P^KG08356/S2P¹

(Amarneh et al., 2009)

NOT Suppressor of

Statement

Reference

Drice^Δ1/Drice[+] is a non-suppressor of visible phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1 is a non-suppressor of decreased cell death phenotype of Dcp-1^Prev1

(Florentin and Arama, 2012)

Other

Statement

Reference

Dcp-1^Prev1, Drice^Δ1 has increased cell death | recessive | P-stage phenotype

(Akagawa et al., 2015)

Dcp-1^Prev1, Drice^Δ1 has lethal | pharate adult stage phenotype

(Lee et al., 2011)

Dcp-1^Prev1, Drice^Δ1 has abnormal developmental rate | pupal stage phenotype

(Lee et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Drice^Δ1 has arista phenotype, enhanceable by Dcp-1[+]/Dcp-1^Prev1

(Muro et al., 2006)

Drice^Δ1 has male genitalia phenotype, enhanceable by Dcp-1[+]/Dcp-1^Prev1

(Muro et al., 2006)

NOT suppressed by

Statement

Reference

Drice^Δ1 has wing disc phenotype, non-suppressible by Dcp-1^Prev1

(Florentin and Arama, 2012)

Suppressor of

Statement

Reference

Drice^Δ1/Drice¹⁷ is a suppressor of eye phenotype of hid^GMR.PG

(Wu et al., 2016)

Drice^Δ1/Drice¹⁷ is a suppressor of eye phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Drice^L1 is a suppressor of eye phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Drice^L2 is a suppressor of eye phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^C1/Drice^Δ1 is a suppressor of eye phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Drice^C2 is a suppressor of eye phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Drice^S1 is a suppressor of eye phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Drice^S2 is a suppressor of eye phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1/Ice[+] is a suppressor of rhabdomere of eye photoreceptor cell phenotype of ninaE¹⁷, ninaE^P37H

(Griciuc et al., 2014)

Drice^Δ1, Dcp-1[+], Dcp-1^Prev1 is a suppressor of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Drice^Δ1/Drice^Δ1 is a suppressor of eye phenotype of hid^GMR.PG

(Muro et al., 2006)

Drice^Δ1/Drice^Δ1 is a suppressor of eye phenotype of grim^GMR.PH

(Muro et al., 2006)

Drice^Δ1/Drice^Δ1 is a suppressor of eye phenotype of rpr^GMR.PH

(Muro et al., 2006)

Drice^Δ1 is a suppressor of arista phenotype of Diap1^th-1

(Muro et al., 2006)

Drice^Δ1 is a suppressor of eye phenotype of Diap1^RNAi.UAS, Scer\GAL4^GMR.PF

(Muro et al., 2006)

Drice^Δ1 is a suppressor of pigment cell phenotype of Diap1^RNAi.UAS, Scer\GAL4^GMR.PF

(Muro et al., 2006)

NOT Suppressor of

Statement

Reference

Drice^Δ1/Drice[+] is a non-suppressor of eye phenotype of rpr^GMR.PW

(Wu et al., 2016)

Drice^Δ1 is a non-suppressor of retina phenotype of Dronc^GMR.PH

(Muro et al., 2006)

Other

Statement

Reference

Dcp-1^Prev1, Drice^Δ1 has adult optic lobe | P-stage phenotype

(Akagawa et al., 2015)

Dcp-1^Prev1, Drice^Δ1 has neuron phenotype

(Lee et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Drice¹⁷/Drice^Δ1 (but not Drice¹⁷/+) significantly suppresses the eye ablation phenotype in hid^GMR.PG flies. Drice¹⁷/Drice^Δ1, Drice^Δ1/Drice^L1, Drice^Δ1/Drice^L2, Drice^Δ1/Drice^C1, Drice^Δ1/Drice^C2, Drice^Δ1/Drice^S1, Drice^Δ1/Drice^S2 (but not Drice^Δ1/+) significantly suppresses the eye ablation phenotype in rpr^GMR.PW flies.

(Wu et al., 2016)

In Drice^Δ1 and Dcp-1^Prev1 double mutants, the number of apoptotic cells throughout the optic lobe is greatly reduced, though a few apoptotic cells are detectable in some regions of the optic lobe at 48h, when in wild type there are no apoptotic cells in these regions.

(Akagawa et al., 2015)

ninaE^P37H, ninaE¹⁷, Ice^Δ1/+ flies show dramatic suppression of retinal degeneration and strong recovery of visual response to light stimulation.

(Griciuc et al., 2014)

Dcp-1^Prev1; Ice^Δ1 double mutants exhibit a block on cell death after irradiation.

A Dcp-1^Prev1; Ice^Δ1 double mutant background fails to abolish the ability of Ice^fl.5'3'UTR mutants to induce cell death, although induction is at a reduced rate compared to in a Ice^Δ1 single mutant background.

The presence of the Dcp-1^Ice.5'3'UTR construct restores larval survival to adulthood in Ice^Δ1 mutants.

Dcp-1^Prev1/+; Ice^Δ1 double mutant third instar larvae exhibit a <10% survival rate to adulthood. Dcp-1^Prev1; Ice^Δ1 double homozygous mutants exhibit lethality at the pupal stage.

The presence of a Ice^fl.5'3'UTR transgene restores third instar larval survival levels to wild-type standards in Dcp-1^Prev1/+; Ice^Δ1 and Dcp-1^Prev1; Ice^Δ1 double mutants.

The presence of a Dcp-1^Ice.5'3'UTR transgene fails to restore third instar larval survival levels to wild-type standards in Dcp-1^Prev1/+; Ice^Δ1 and Dcp-1^Prev1; Ice^Δ1 double mutants.

The presence of a Dcp-1^fl.5'3'UTR transgene partially restores the levels of apoptosis seen after irradiation in Ice^Δ1 mutant imaginal wing discs.

The presence of a Dcp-1^fl.5'3'UTR transgene fails to affect the abolition of apoptosis seen after irradiation in Dcp-1^Prev1/+; Ice^Δ1 or Dcp-1^Prev1; Ice^Δ1 double mutant imaginal wing discs.

(Florentin and Arama, 2012)

Dcp-1^Prev1; Ice^Δ1 double mutants retain all 16 vCrz neurons at 7 and 16 hours after puparium formation, compared to apoptosis of these neurons in wild-type controls. Dcp-1^Prev1; Ice^Δ1 double mutants display developmental arrest around the mid-pupal stage.

(Lee et al., 2011)

S2P^KG08356/S2P¹, Ice^Δ1/+ and S2P^KG08356/S2P¹, Ice^Δ1/Ice^Δ1 mutant larvae exhibit lethality between second and third instar stages. Lethality is rescued partially by supplementing fly food with fatty acids, but viability is still reduced compared to S2P^KG08356/S2P¹ mutants.

(Amarneh et al., 2009)

Dcp-1^Prev1 ; Nc^I24/Nc^I29 Ice^Δ1 triple mutant larvae undergo cell death with morphology similar to the midgut of wild-type animals when analysed from -4 to -1 hours relative to puparium formation.

dream⁴ ; Nc^I24/Nc^I29 Ice^Δ1 triple mutant larvae show high levels of TUNEL staining at -4 to -1 hours relative to puparium formation, suggesting that they are undergoing cell death.

Pupae expressing decay^GD8484 under the control of Scer\GAL4^NP1 in a Ice^Δ1 Nc^unspecified background undergo midgut histolysis at a similar rate as wild-type animals.

(Denton et al., 2009)

The lethality of Ice^Δ1 is enhanced by Dcp-1^Prev1 : less than 1% of Dcp-1^Prev1/+, Ice^Δ1/Ice^Δ1 animals eclose and Dcp-1^Prev1/Dcp-1^Prev1; Ice^Δ1/Ice^Δ1 animals die prior to, or during, early pupal stages. Almost all Dcp-1^Prev1/Dcp-1^Prev1; Ice^Δ1/Ice^Δ1 pupae arrest development prior to head inversion.

Ice^Δ1 suppresses the loss of lateral shafts on the arista of th¹ flies.

Dcp-1^Prev1/+, Ice^Δ1 flies show an increased number of lateral branches on the arista compared to Ice^Δ1 single mutant flies. Additionally, the genitalia misrotation phenotype of Ice^Δ1 flies is increased to full penetrance in Dcp-1^Prev1/+, Ice^Δ1 flies.

The small eye phenotypes exhibited by flies expressing grim^GMR.PH and rpr^GMR.PH are suppressed in a Ice^Δ1 homozygous background, while the small eye phenotype of W^GMR.PG flies is only partially suppressed by Ice^Δ1.

An Ice^Δ1 background does not significantly suppress the retinal degeneration phenotype of Nc^GMR.PH flies.

The small eye phenotype of Scer\GAL4^GMR.PF>th^{dsRNA.Scer\UAS} flies is partially suppressed in an Ice^Δ1 background.

(Muro et al., 2006)

Xenogenetic Interactions

Statement

Reference

A Dcp-1^Prev1/+; Ice^Δ1/Ice^Δ1 background strongly suppresses the adult eye size defect found upon expression of Mmus\Gria1^Lc.Scer\UAS under the control of Scer\GAL4^hs.2sev.

(Yang et al., 2013)

Complementation and Rescue Data

Rescued by

Drice^Δ1 is rescued by Drice^fl.5'3'UTR

(Florentin and Arama, 2012)

Drice^Δ1 is rescued by Drice^+t2.4

(Muro et al., 2006)

Not rescued by

Drice^Δ1 is not rescued by Drice^{Dcp-1.5'3'UTR}

(Florentin and Arama, 2012)

Comments

The presence of the Ice^fl.5'3'UTR construct restores cell death after irradiation in Ice^Δ1 mutants.

The presence of the Ice^{Dcp-1.5'3'UTR} construct fails to restore wild-type levels of cell death after irradiation in Ice^Δ1 mutant imaginal wing discs.

The presence of the Ice^fl.5'3'UTR construct restores larval survival to adulthood in Ice^Δ1 mutants.

(Florentin and Arama, 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

DrICE^Δ1

(McSharry and Beitel, 2019)

Drice^Δ1

(Tavignot et al., 2017)

Ice^LOF

(Griciuc et al., 2014)

Ice^Δ1

(Lee et al., 2011, Muro et al., 2006)

IceΔ1

(Griciuc et al., 2014)

drICE^Delta1

(Yang et al., 2013)

drICE^Δ1

(Amcheslavsky et al., 2018, Wu et al., 2016, Akagawa et al., 2015)

drice^Δ1

(Tarayrah-Ibraheim et al., 2021, Lee et al., 2013, Yacobi-Sharon et al., 2013, Florentin and Arama, 2012, Schoenmann et al., 2010, Amarneh et al., 2009, Denton et al., 2009, Link et al., 2007)

ice^Δ1

(Lee et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (18)

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