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General Information
Symbol
Dmel\DriceΔ1
Species
D. melanogaster
Name
FlyBase ID
FBal0193685
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
IceΔ1
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{EP} element, resulting in deletion of the entire Ice coding region and fusion of the Ice 5' and 3' UTR sequences. A 1.5kb fragment of the P{EP} element remains.

Insertion components
P{?EP}DriceΔ1
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In DriceΔ1 mutants, the peak number of apoptotic cells in the optic lobe is delayed to 36h APF and the number of apoptotic cells is increased relative to wild type. In addition, the spatial distribution of apoptotic cells throughout different regions of the optic lobe is significantly different compared to wild type. Cell corpse clearance is decreased throughout the optic lobe in regions of cell death, as compared to wild type.

The abdominal ganglion where axonal bundles exit to innervate abdominal tissue is substantially elongated and broader in homozygous IceΔ1 mutants. The programmed cell death of bursCCAP neurons seen in the adult ventral nerve cord of wild type flies after eclosion is strongly suppressed; six bursCCAP neurons survive in 3-5 day old IceΔ1 mutant flies.

The programmed cell death of bursCCAP neurons seen in the adult ventral nerve cord of wild type flies after eclosion is strongly suppressed in IceΔ1/Ice17 mutants; six bursCCAP neurons survive in 3-5 day old flies.

Levels of germ cell death are slightly increased in IceΔ1 mutant spermatogonial cysts.

IceΔ1 mutant wing discs display a complete block in cell death, even after 50 Gy irradiation.

IceΔ1 mutant larva exhibit a <20% survival rate to adulthood.

All 16 vCrz neurons are present at 7 hours after puparium formation in IceΔ1 mutants. The surviving neurons display budding structures similar to those of dying neurons in wild-type flies at 1-2 hours after puparium formation. The number of vCrz neurons is progressively reduced to 13 at 12-16 hours after puparium formation and 7 at 24 hours after puparium formation.

Ice17/IceΔ1 trans-heterozygous mutants exhibit a reduction in dendritic branch pruning at 18 hours after puparium formation.

IceΔ1 homozygous adult 'escapers' present normal wings at eclosion but develop blemishes with age.

80% of IceΔ1 mutants die following puparium formation. A similar survival rate is observed for IceΔ1 mutants derived from homozygous IceΔ1 mothers.

Most (73%) of homozygous IceΔ1 pupae appear similar to controls after puparium formation. The remaining 27% are arrested in development. Of these arrested pupae, one-third fail to undergo head eversion and arrest prior to the commencement of salivary gland cell death. The other two thirds appear to have arrested 1 or 2 hours after head eversion, and thus the salivary glands and larval muscle are not degraded, and the larval gut has failed to condense properly. All IceΔ1 pupae possess many abnormal masses in the head, abdomen, wing disc and leg disc. The frequency of these masses are increased by almost two-fold when IceΔ1 animals are subjected to X-irradiation during larval stages.

The arista of IceΔ1 flies has a much thicker central shaft and many more lateral branches than the arista of wild-type flies. The genitalia of approximately 50% of IceΔ1 male adults is markedly rotated compared to wild-type males. Additionally, more than 50% of IceΔ1 adults have an open 'scar' of varying severity along the dorsal abdominal midline. The tissue appears thin and fragile, with some flies possessing holes or tears in this area associated with leakage of hemolymph.

IceΔ1 flies have opaque wings, which sometimes contain trapped fluid or are not fully extended. Visualization of cell death in IceΔ1 wings shows that cells persist in the posterior compartment of the wing that have died in the posterior compartment of wild-type wings.

The retinae of IceΔ1 flies show on average three additional interommatidial cells per ommatidia compared to wild-type retinae in which cell death eliminates excess interommatidial cells.

IceΔ1 embryos show lower levels of cell death than wild-type embryos, according to TUNEL labeling. In addition, stage 17 IceΔ1 embryos contain on average 6 midline glia per segment while wild-type embryos contain only 3 midline glia per segment.

The wing discs of IceΔ1 flies do not show increased cell death response to irradiation that wild-type discs show. IceΔ1 hemocytes do not die in response to cycloheximide as occurs in wild-type cells.

The spermatid cysts of IceΔ1 testis show a partial failure in individualization.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

DriceΔ1 has lethal | pupal stage phenotype, enhanceable by Dcp-1[+]/Dcp-1Prev1

NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference

DriceΔ1/Drice17 is a suppressor of visible phenotype of hidGMR.PG

DriceΔ1/Drice17 is a suppressor of visible phenotype of rprGMR.PW

DriceΔ1/DriceL1 is a suppressor of visible phenotype of rprGMR.PW

DriceΔ1/DriceL2 is a suppressor of visible phenotype of rprGMR.PW

DriceC1/DriceΔ1 is a suppressor of visible phenotype of rprGMR.PW

DriceΔ1/DriceC2 is a suppressor of visible phenotype of rprGMR.PW

DriceΔ1/DriceS1 is a suppressor of visible phenotype of rprGMR.PW

DriceΔ1/DriceS2 is a suppressor of visible phenotype of rprGMR.PW

DriceΔ1/Ice[+] is a suppressor of abnormal neuroanatomy phenotype of ninaE17, ninaEP37H

DriceΔ1/Ice[+] is a suppressor of viable | conditional phenotype of S2PKG08356/S2P1

NOT Suppressor of
Statement
Reference

DriceΔ1/Drice[+] is a non-suppressor of visible phenotype of rprGMR.PW

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

DriceΔ1 has arista phenotype, enhanceable by Dcp-1[+]/Dcp-1Prev1

DriceΔ1 has male genitalia phenotype, enhanceable by Dcp-1[+]/Dcp-1Prev1

NOT suppressed by
Statement
Reference

DriceΔ1 has wing disc phenotype, non-suppressible by Dcp-1Prev1

Suppressor of
Statement
Reference

DriceΔ1/Drice17 is a suppressor of eye phenotype of hidGMR.PG

DriceΔ1/Drice17 is a suppressor of eye phenotype of rprGMR.PW

DriceΔ1/DriceL1 is a suppressor of eye phenotype of rprGMR.PW

DriceΔ1/DriceL2 is a suppressor of eye phenotype of rprGMR.PW

DriceC1/DriceΔ1 is a suppressor of eye phenotype of rprGMR.PW

DriceΔ1/DriceC2 is a suppressor of eye phenotype of rprGMR.PW

DriceΔ1/DriceS1 is a suppressor of eye phenotype of rprGMR.PW

DriceΔ1/DriceS2 is a suppressor of eye phenotype of rprGMR.PW

DriceΔ1, Dcp-1[+], Dcp-1Prev1 is a suppressor of eye phenotype of Mmus\Gria1Lc.UAS, Scer\GAL4hs.2sev

DriceΔ1/DriceΔ1 is a suppressor | partially of eye phenotype of hidGMR.PG/WGMR.PG

DriceΔ1/DriceΔ1 is a suppressor of eye phenotype of grimGMR.PH

DriceΔ1 is a suppressor of arista phenotype of Diap11

DriceΔ1/DriceΔ1 is a suppressor of eye phenotype of rprGMR.PH

NOT Suppressor of
Statement
Reference

DriceΔ1/Drice[+] is a non-suppressor of eye phenotype of rprGMR.PW

DriceΔ1 is a non-suppressor of retina phenotype of DroncGMR.PH

Other
Additional Comments
Genetic Interactions
Statement
Reference

Drice17/DriceΔ1 (but not Drice17/+) significantly suppresses the eye ablation phenotype in hidGMR.PG flies. Drice17/DriceΔ1, DriceΔ1/DriceL1, DriceΔ1/DriceL2, DriceΔ1/DriceC1, DriceΔ1/DriceC2, DriceΔ1/DriceS1, DriceΔ1/DriceS2 (but not DriceΔ1/+) significantly suppresses the eye ablation phenotype in rprGMR.PW flies.

In DriceΔ1 and Dcp-1Prev1 double mutants, the number of apoptotic cells throughout the optic lobe is greatly reduced, though a few apoptotic cells are detectable in some regions of the optic lobe at 48h, when in wild type there are no apoptotic cells in these regions.

ninaEP37H, ninaE17, IceΔ1/+ flies show dramatic suppression of retinal degeneration and strong recovery of visual response to light stimulation.

Dcp-1Prev1; IceΔ1 double mutants exhibit a block on cell death after irradiation.

A Dcp-1Prev1; IceΔ1 double mutant background fails to abolish the ability of Icefl.5'3'UTR mutants to induce cell death, although induction is at a reduced rate compared to in a IceΔ1 single mutant background.

The presence of the Dcp-1Ice.5'3'UTR construct restores larval survival to adulthood in IceΔ1 mutants.

Dcp-1Prev1/+; IceΔ1 double mutant third instar larvae exhibit a <10% survival rate to adulthood. Dcp-1Prev1; IceΔ1 double homozygous mutants exhibit lethality at the pupal stage.

The presence of a Icefl.5'3'UTR transgene restores third instar larval survival levels to wild-type standards in Dcp-1Prev1/+; IceΔ1 and Dcp-1Prev1; IceΔ1 double mutants.

The presence of a Dcp-1Ice.5'3'UTR transgene fails to restore third instar larval survival levels to wild-type standards in Dcp-1Prev1/+; IceΔ1 and Dcp-1Prev1; IceΔ1 double mutants.

The presence of a Dcp-1fl.5'3'UTR transgene partially restores the levels of apoptosis seen after irradiation in IceΔ1 mutant imaginal wing discs.

The presence of a Dcp-1fl.5'3'UTR transgene fails to affect the abolition of apoptosis seen after irradiation in Dcp-1Prev1/+; IceΔ1 or Dcp-1Prev1; IceΔ1 double mutant imaginal wing discs.

Dcp-1Prev1; IceΔ1 double mutants retain all 16 vCrz neurons at 7 and 16 hours after puparium formation, compared to apoptosis of these neurons in wild-type controls. Dcp-1Prev1; IceΔ1 double mutants display developmental arrest around the mid-pupal stage.

S2PKG08356/S2P1, IceΔ1/+ and S2PKG08356/S2P1, IceΔ1/IceΔ1 mutant larvae exhibit lethality between second and third instar stages. Lethality is rescued partially by supplementing fly food with fatty acids, but viability is still reduced compared to S2PKG08356/S2P1 mutants.

Dcp-1Prev1 ; NcI24/NcI29 IceΔ1 triple mutant larvae undergo cell death with morphology similar to the midgut of wild-type animals when analysed from -4 to -1 hours relative to puparium formation.

dream4 ; NcI24/NcI29 IceΔ1 triple mutant larvae show high levels of TUNEL staining at -4 to -1 hours relative to puparium formation, suggesting that they are undergoing cell death.

Pupae expressing decayGD8484 under the control of Scer\GAL4NP1 in a IceΔ1 Ncunspecified background undergo midgut histolysis at a similar rate as wild-type animals.

The lethality of IceΔ1 is enhanced by Dcp-1Prev1 : less than 1% of Dcp-1Prev1/+, IceΔ1/IceΔ1 animals eclose and Dcp-1Prev1/Dcp-1Prev1; IceΔ1/IceΔ1 animals die prior to, or during, early pupal stages. Almost all Dcp-1Prev1/Dcp-1Prev1; IceΔ1/IceΔ1 pupae arrest development prior to head inversion.

IceΔ1 suppresses the loss of lateral shafts on the arista of th1 flies.

Dcp-1Prev1/+, IceΔ1 flies show an increased number of lateral branches on the arista compared to IceΔ1 single mutant flies. Additionally, the genitalia misrotation phenotype of IceΔ1 flies is increased to full penetrance in Dcp-1Prev1/+, IceΔ1 flies.

The small eye phenotypes exhibited by flies expressing grimGMR.PH and rprGMR.PH are suppressed in a IceΔ1 homozygous background, while the small eye phenotype of WGMR.PG flies is only partially suppressed by IceΔ1.

An IceΔ1 background does not significantly suppress the retinal degeneration phenotype of NcGMR.PH flies.

The small eye phenotype of Scer\GAL4GMR.PF>thdsRNA.Scer\UAS flies is partially suppressed in an IceΔ1 background.

Xenogenetic Interactions
Statement
Reference

A Dcp-1Prev1/+; IceΔ1/IceΔ1 background strongly suppresses the adult eye size defect found upon expression of Mmus\Gria1Lc.Scer\UAS under the control of Scer\GAL4hs.2sev.

Complementation and Rescue Data
Not rescued by
Comments

The presence of the Icefl.5'3'UTR construct restores cell death after irradiation in IceΔ1 mutants.

The presence of the IceDcp-1.5'3'UTR construct fails to restore wild-type levels of cell death after irradiation in IceΔ1 mutant imaginal wing discs.

The presence of the Icefl.5'3'UTR construct restores larval survival to adulthood in IceΔ1 mutants.

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Wild-type
Stocks (0)
Notes on Origin
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External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (18)