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General Information
Symbol
Dmel\Gr63a1
Species
D. melanogaster
Name
FlyBase ID
FBal0193768
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ΔGr63a
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The entire Gr63a gene has been replaced by a w+* marker.

Insertion components
TI{TI}Gr63a1
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Gr63a1 homozygotes do not show avoidance to CO2, unlike wild-type and heterozygous controls.

Female Gr63a1 homozygotes have up to a 30% increase in lifespan compared to controls. The lifespan of male homozygotes does not differ from wild type. Heterozygous Gr63a1 females have a similar lifespan to controls.

Gr63a1 homozygous females are of similar weight and size to controls and contain a similar level of proteins. However triglyceride levels are elevated in homozygous Gr63a1 females compared to wild type. No change in triglyceride levels is observed in heterozygotes.

Female Gr63a1 homozygous mutants do not show an increase in lifespan under starvation conditions compared to wild type.

Female Gr63a1 mutants lay approximately 40% more eggs over a two week period than control flies.

Female Gr63a1 flies show increased oxidative stress resistance compared to wild type females (when exposed to methyl viologen). No difference from wild type is seen in heterozygous flies.

No changes in circadian rhythm or activity levels are seen in Gr63a1 mutants. A reduction in sleep is observed but is not reproducible in all experiments (seen in 1 out of 3).

Dietary restriction enhances the increase longevity phenotype seen in female Gr63a1 mutants. The magnitude of the increase is similar to the increase in longevity seen in dietary restricted wild type flies. There is no evidence of alteration to food consumption levels in Gr63a1 female flies compared to controls.

Gr63a1 female mutants are less able to detect live yeast than control flies. Unlike wild type flies Gr63a1 mutants do not show a preference for live yeast over autoclaved yeast.

Unlike in wild type, Gr63a1 mutants do not exhibit a reduction in lifespan in response to the odour of live yeast, and in one out of four experiments lifespan slightly increases compared to controls. No alteration to lifespan occurs in either wild type or Gr63a1 flies when they are exposed to yeast killed by autoclaving.

Electrophysiological recordings of ab1 sensilla in Gr63a1 flies reveal a complete indifference to CO2 stimuli, while ab1C neurons in wild-type flies do respond to CO2. This phenotype is not seen in Gr63a1/+ flies. Further, Gr63a1 flies fail to avoid CO2 in a T maze, while Gr63a1/+ flies show a wild-type behavioral response to CO2.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of both Gr63aScer\UAS.cJa and Gr21aScer\UAS.cJa under the control of Scer\GAL4Ir64a.PA in Ir64aMB05283, Gr63a1 double mutant flies allows artificial stimulation of DC4 dorsal glomeruli of the adult antennal lobe by 5% CO[[2]] and robust avoidance of CO[[2]] in behavioural experiments. However, DP1m neurons are not activated by CO[[2]] in these mutants.

Behavioural experiments demonstrate that Gr63a1; Ir64aMB05283 double mutant flies fail to distinguish ambient air from 5% CO[[2]].

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of Gr63aScer\UAS.cJa under the control of the native promoter (Scer\GAL4[Gr63a.PF]) restores the increased lifespan phenotype of Gr63a1 females to wild type levels.

Expression of Gr63aScer\UAS.cJa under the control of the Scer\GAL4Gr63a.PF driver rescues the high triglyceride level phenotype seen in Gr63a1 female mutants.

Expression of Gr63aScer\UAS.cJa under the control of Scer\GAL4Gr21a.9.323 rescues the ability of ab1C neurons to respond to CO2 in Gr63a1 flies and partially rescues the ability of Gr63a1 flies to avoid CO2 in a T-maze.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (8)