FlyBase Allele Report: Dmel\car[Δ146]

General Information

Symbol

Dmel\car^Δ146

Species

D. melanogaster

Name

FlyBase ID

FBal0194558

Feature type

allele

Associated gene

Dmel\car

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Akbar et al., 2007)

Mutagen

P-element activity

(Akbar et al., 2009)

Progenitor genotype

P{lacW}car^G0447

(Akbar et al., 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes almost the entire car coding sequence (sequences encoding amino acid residues 1-584 out of 617 residues are removed), without disrupting neighbouring genes..

(Akbar et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:19,566,192..19,568,215 [+]

Comment:

Approximate location of deletion resulting from the imprecise excision of P{lacW}car^G0447. The deletion extends from the insertion site (~150 bases upstream of the car ATG through codon 584.

(Akbar et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of second instar larval stage | recessive

(Akbar et al., 2009)

some die during second instar larval stage | recessive

(Akbar et al., 2009)

visible | recessive | somatic clone

(Akbar et al., 2009)

Phenotype Manifest In

ommatidium | somatic clone

(Akbar et al., 2009)

pigment cell | somatic clone

(Akbar et al., 2009)

rhabdomere | cell autonomous | somatic clone

(Akbar et al., 2009)

wing | somatic clone

(Akbar et al., 2009)

Detailed Description

Statement

Reference

Homozygous mushroom body gamma neuron clones show normal axon pruning.

(Issman-Zecharya and Schuldiner, 2014)

Homozygotes do not develop beyond the second larval instar, although the second instar larvae can survive up to 6 days from the day of egg laying.

Homozygous clones in the eye lack almost all pigmentation. All photoreceptor cells are present in the mutant ommatidia and are arranged in their normal trapezoidal configuration. The rhabdomeres in the mutant ommatidia are smaller than normal (this phenotype is cell-autonomous) in 2 day old adults, and the mutant photoreceptors have an extensive accumulation of vacuoles, many of which have the double membranes typical of autophagosomes.

Homozygous clones in the eyes of 20 day old flies show extensive degeneration of retinal cells, with most cellular structures, including rhabdomeres, being barely recognisable. This phenotype is not light dependent.

2 day old flies with homozygous clones in the wing show a darkening of the wing as the first sign of degeneration. In older flies, the wings start to become frayed and end up notched.

(Akbar et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

car^Δ146 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

car^Δ146 has pigment cell | somatic clone phenotype, non-suppressible | somatic clone by Scer\GAL4^Tub.PU/Vps33B^UAS.Tag:MYC

(Akbar et al., 2009)

car^Δ146 has eye | somatic clone phenotype, non-suppressible | somatic clone by Scer\GAL4^Tub.PU/Vps33B^UAS.Tag:MYC

(Akbar et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Vps33B^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tub in car^Δ146 clones in the eye does not restore pigmentation in the clones and does not prevent the late degeneration seen in single mutant car^Δ146 patches in the eye.

(Akbar et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

car¹

(Akbar et al., 2009)

Rescued by

car^Δ146 is rescued by car^+t4.4

(Akbar et al., 2009)

car^Δ146 is rescued by car^UAS.cAa/Scer\GAL4^da.G32

(Akbar et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

car^d146

(Takáts et al., 2014)

car^Δ146

(Jin et al., 2018, Issman-Zecharya and Schuldiner, 2014, Walker et al., 2013, Akbar et al., 2009, Akbar et al., 2007)

car^Δ

(Shimizu et al., 2017)

car[d146]

(Takáts et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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