Homozygous mushroom body gamma neuron clones show normal axon pruning.
Homozygotes do not develop beyond the second larval instar, although the second instar larvae can survive up to 6 days from the day of egg laying.
Homozygous clones in the eye lack almost all pigmentation. All photoreceptor cells are present in the mutant ommatidia and are arranged in their normal trapezoidal configuration. The rhabdomeres in the mutant ommatidia are smaller than normal (this phenotype is cell-autonomous) in 2 day old adults, and the mutant photoreceptors have an extensive accumulation of vacuoles, many of which have the double membranes typical of autophagosomes.
Homozygous clones in the eyes of 20 day old flies show extensive degeneration of retinal cells, with most cellular structures, including rhabdomeres, being barely recognisable. This phenotype is not light dependent.
2 day old flies with homozygous clones in the wing show a darkening of the wing as the first sign of degeneration. In older flies, the wings start to become frayed and end up notched.