FlyBase Allele Report: Dmel\Pink1[RNAi.UAS.cWa]

General Information

Symbol

Dmel\Pink1^RNAi.UAS.cWa

Species

D. melanogaster

Name

FlyBase ID

FBal0196597

Feature type

allele

Associated gene

Dmel\Pink1

Associated Insertion(s)

Carried in Construct

P{UAS-Pink1.RNAi.W}

Also Known As

dPINK1-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Wang et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Wang et al., 2006)

Mutagen

in vitro construct

(Wang et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Pink1.RNAi.W}

Cytology

Description

UAS regulatory sequences drive expression of two copies of a Pink1 PCR fragment (derived from a cDNA and corresponding to base pairs 1410 to 1727 of Pink1) arranged in opposite orientations.

(Wang et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pink1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Wang et al., 2006)

CEC

(Choo et al., 2012)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease

is ameliorated by Hsap\SOD1^UAS.cPa

(Wang et al., 2006)

is ameliorated by APP-BP1^UAS.cKa

(Choo et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dopamine neuron & adult brain, with Scer\GAL4^Ddc.PL

(Wang et al., 2006)

dopamine neuron & adult brain, with Scer\GAL4^elav.PLu

(Wang et al., 2006)

eye, with Scer\GAL4^elav.PLu

(Wang et al., 2006)

eye, with Scer\GAL4^GMR.PF

(Choo et al., 2012, Wang et al., 2006)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Venderova et al., 2009, Wang et al., 2006)

ommatidium, with Scer\GAL4^elav.PLu

(Wang et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Wang et al., 2006)

photoreceptor & neuron, with Scer\GAL4^GMR.PF

(Wang et al., 2006)

pigment cell, with Scer\GAL4^GMR.PF

(Venderova et al., 2009, Wang et al., 2006)

Detailed Description

Statement

Reference

Expression of Pink1^{dsRNA.Scer\UAS.cWa} under the control of Scer\GAL4^GMR.PF results in ommatidial degeneration resulting in black speckles, patches or lesions in the eyes.

(Choo et al., 2012)

Flies expressing Pink1^{dsRNA.Scer\UAS.cWa} under the control of Scer\GAL4^GMR.PF at room temperature have a mutant eye phenotype characterised by pigmentation deficits and bristle loss. The flies also have black lesions in the eyes.

(Venderova et al., 2009)

Adult brains of 1 day-old flies expressing Pink1^{Scer\UAS.dsRNA.cWa} under the control of Scer\GAL4^elav.PLu show little difference in the number and distribution of dopaminergic (DA) neurons compared to controls. In contrast, mutant flies show a dramatic reduction in DA neuron number at 10 days of age, compared to age-matched controls: significant neuronal loss is seen in most DA neuron clusters including in the PAL, PPM1/2, PPM3, PPL2 and less so in PPL1, although the number of DA neurons in the VUM regions are not significantly affected. (Staining of serotonergic neurons shows little difference between Pink1^{Scer\UAS.dsRNA.cWa}-expressing and control flies.) A similar loss of DA neurons is seen in the adult brains of flies expressing Pink1^{Scer\UAS.dsRNA.cWa} under the control of Scer\GAL4^Ddc.PL.

Expression of Pink1^{Scer\UAS.dsRNA.cWa} under the control of Scer\GAL4^GMR.PF or Scer\GAL4^elav.PLu results in age-dependent progressive ommatidial degeneration manifested in black lesions in the adult eyes. These eyes are also 'rough', with disorganized interommatidial bristles. Pink1^{Scer\UAS.dsRNA.cWa}, Scer\GAL4^GMR.PF pupal eyes (observed at 44 hours APF) exhibit morphologically disrupted ommatidia, increased and misorientated mechanosensory bristle groups, disorganized and enlarged pigment cells, and a significant loss of photoreceptor neurons compared to controls.

Treatment with antioxidants (recombinant SOD1 protein or vitamin E) inhibits the ommatidial degeneration defects in flies expressing Pink1^{Scer\UAS.dsRNA.cWa} under the control of Scer\GAL4^GMR.PF.

(Wang et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by APP-BP1^UAS.cKa, Scer\GAL4^GMR.PF

(Choo et al., 2012)

Pink1^RNAi.UAS.cWa, Scer\GAL4^da.G32 has lethal phenotype, suppressible by Hsap\PINK1^UAS.Tag:FLAG, Scer\GAL4^da.G32

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^elav.PLu has abnormal neuroanatomy phenotype, suppressible by Hsap\PINK1^UAS.Tag:FLAG, Scer\GAL4^elav.PLu

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^Ddc.PL has abnormal neuroanatomy phenotype, suppressible by Hsap\PINK1^UAS.Tag:FLAG, Scer\GAL4^Ddc.PL

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^elav.PLu has abnormal neuroanatomy phenotype, suppressible by Hsap\SOD1^UAS.cPa, Scer\GAL4^elav.PLu

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Hsap\PINK1^UAS.Tag:FLAG, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Hsap\SOD1^UAS.cPa, Scer\GAL4^GMR.PF

(Wang et al., 2006)

NOT suppressed by

Statement

Reference

Pink1^RNAi.UAS.cWa, Scer\GAL4^da.G32 has lethal phenotype, non-suppressible by Hsap\PINK1^{G309D.UAS.Tag:FLAG}, Scer\GAL4^da.G32

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Hsap\PINK1^{G309D.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by APP-BP1^UAS.cKa, Scer\GAL4^GMR.PF

(Choo et al., 2012)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Hsap\PINK1^UAS.Tag:FLAG, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Hsap\PINK1^UAS.Tag:FLAG, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Hsap\SOD1^UAS.cPa, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Hsap\SOD1^UAS.cPa, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^elav.PLu has dopamine neuron & adult brain phenotype, suppressible by Hsap\PINK1^UAS.Tag:FLAG, Scer\GAL4^elav.PLu

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^Ddc.PL has dopamine neuron & adult brain phenotype, suppressible by Hsap\PINK1^UAS.Tag:FLAG, Scer\GAL4^Ddc.PL

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^elav.PLu has dopamine neuron & adult brain phenotype, suppressible by Hsap\SOD1^UAS.cPa, Scer\GAL4^elav.PLu

(Wang et al., 2006)

NOT suppressed by

Statement

Reference

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Hsap\PINK1^{G309D.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Hsap\PINK1^{G309D.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of APP-BP1^Scer\UAS.cKa partially suppresses the eye phenotype seen when Pink1^{dsRNA.Scer\UAS.cWa} is expressed under the control of Scer\GAL4^GMR.PF.

(Choo et al., 2012)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\PINK1^{Scer\UAS.T:Zzzz\FLAG}, but not Hsap\PINK1^{G309D.Scer\UAS.T:Zzzz\FLAG}, rescues the ommatidial degeneration phenotype of Pink1^{Scer\UAS.dsRNA.cWa}, Scer\GAL4^GMR.PF flies.

(Wang et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Expression of Pink1^{Scer\UAS.dsRNA.cWa} produces dsRNA which results in dsRNA interference (RNAi) of the Pink1 gene.

(Wang et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pink1^RNAi.UAS.cWa

Pink1^{Scer\UAS.dsRNA.cWa}

Pink1^{dsRNA.Scer\UAS.cWa}

Pink1^{dsRNA.UAS.cWa}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close