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General Information
Symbol
Dmel\Pink1dsRNA.UAS.cWa
Species
D. melanogaster
Name
FlyBase ID
FBal0196597
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dPINK1-RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of two copies of a Pink1 PCR fragment (derived from a cDNA and corresponding to base pairs 1410 to 1727 of Pink1) arranged in opposite orientations.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Pink1dsRNA.Scer\UAS.cWa under the control of Scer\GAL4GMR.PF results in ommatidial degeneration resulting in black speckles, patches or lesions in the eyes.

Flies expressing Pink1dsRNA.Scer\UAS.cWa under the control of Scer\GAL4GMR.PF at room temperature have a mutant eye phenotype characterised by pigmentation deficits and bristle loss. The flies also have black lesions in the eyes.

Adult brains of 1 day-old flies expressing Pink1Scer\UAS.dsRNA.cWa under the control of Scer\GAL4elav.PLu show little difference in the number and distribution of dopaminergic (DA) neurons compared to controls. In contrast, mutant flies show a dramatic reduction in DA neuron number at 10 days of age, compared to age-matched controls: significant neuronal loss is seen in most DA neuron clusters including in the PAL, PPM1/2, PPM3, PPL2 and less so in PPL1, although the number of DA neurons in the VUM regions are not significantly affected. (Staining of serotonergic neurons shows little difference between Pink1Scer\UAS.dsRNA.cWa-expressing and control flies.) A similar loss of DA neurons is seen in the adult brains of flies expressing Pink1Scer\UAS.dsRNA.cWa under the control of Scer\GAL4Ddc.PL.

Expression of Pink1Scer\UAS.dsRNA.cWa under the control of Scer\GAL4GMR.PF or Scer\GAL4elav.PLu results in age-dependent progressive ommatidial degeneration manifested in black lesions in the adult eyes. These eyes are also 'rough', with disorganized interommatidial bristles. Pink1Scer\UAS.dsRNA.cWa, Scer\GAL4GMR.PF pupal eyes (observed at 44 hours APF) exhibit morphologically disrupted ommatidia, increased and misorientated mechanosensory bristle groups, disorganized and enlarged pigment cells, and a significant loss of photoreceptor neurons compared to controls.

Treatment with antioxidants (recombinant SOD1 protein or vitamin E) inhibits the ommatidial degeneration defects in flies expressing Pink1Scer\UAS.dsRNA.cWa under the control of Scer\GAL4GMR.PF.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Phenotype Manifest In
Suppressed by
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

Expression of APP-BP1Scer\UAS.cKa partially suppresses the eye phenotype seen when Pink1dsRNA.Scer\UAS.cWa is expressed under the control of Scer\GAL4GMR.PF.

Xenogenetic Interactions
Statement
Reference

Co-expression of Hsap\PINK1Scer\UAS.T:Zzzz\FLAG, but not Hsap\PINK1G309D.Scer\UAS.T:Zzzz\FLAG, rescues the ommatidial degeneration phenotype of Pink1Scer\UAS.dsRNA.cWa, Scer\GAL4GMR.PF flies.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Expression of Pink1Scer\UAS.dsRNA.cWa produces dsRNA which results in dsRNA interference (RNAi) of the Pink1 gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Pink1Scer\UAS.dsRNA.cWa
Pink1dsRNA.Scer\UAS.cWa
Pink1dsRNA.UAS.cWa
Name Synonyms
Secondary FlyBase IDs
    References (4)