FlyBase Allele Report: Hsim\VP16[AD.elav]

General Information

Symbol

Hsim\VP16^AD.elav

Species

S. Herpes simplex virus Type 1

Name

FlyBase ID

FBal0198078

Feature type

allele

Associated gene

Hsim\VP16

Associated Insertion(s)

Carried in Construct

P{elav-VP16.AD}

Also Known As

elav-VP16AD

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Luan et al., 2006)

in vitro construct - coding region fusion

(Luan et al., 2006)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{elav-VP16.AD}

Cytology

Nature of the lesion

Statement

Reference

An elav promoter regulates expression of a VP16(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the VP16 activation domain). The hemidriver sequence includes the Tag:NLS(SV40-largeT) nuclear localization signal. An internal ribosomal entry site (IRES) from the Ubx gene followed by DsRed(Unk) coding sequence is present downstream of the hemidriver sequence, but this is not expressed in the nervous system.

(Luan et al., 2006)

Allele components

Product class / Tool use(s)

Regulatory region(s)

elav

Encoded product / tool

VP16(AD)::Zip+

split driver - transcription activation fragment

Tagged with

Tag:NLS(SV40-largeT)

nuclear protein localization tag

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval central nervous system

Comment: when combined with P{elav-GAL4.DBD}

(Luan et al., 2006)

adult stage

adult doublesex aDN neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult stage | female

adult doublesex AbN (female) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult doublesex pCd (female) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult fruitless pIP-e (female) neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult fruitless pMP-e (female) neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

epiproct | restricted

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

hypogynium | restricted

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

hypoproct | restricted

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

mechanosensory neuron | subset of female terminalia

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult stage | male

adult doublesex AbN (male) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult doublesex SN (male) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult doublesex TN1 (male) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult doublesex TN2 (male) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult doublesex pCd (male) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult fruitless pIP-e (male) neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult fruitless P1 (male) neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

cercus | restricted

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

surstylar tooth | restricted

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

hypandrial bristle | restricted

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

epandrial ventral lobe | restricted

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

mechanosensory neuron | subset of male terminalia

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

Additional Information

Statement

Reference

Hsim\VP16^AD.elav drives expression specifically in neurons when combined with Scer\GAL4^dsx-GAL4-DBD in a split-GAL4 system including mechanosensory neurons in the terminalia associated with the bristles of the clasper tooth, lateral plates and anal plates in males, and the anal and vaginal plates in females.

(Pavlou et al., 2016)

Hsim\VP16^AD.elav drives expression pan-neuronally, recapitulating the elav expression pattern, when combined with Scer\GAL4^DBD.elav in a split-GAL4 system.

(Luan et al., 2006)

Marker for

Reflects expression of

elav

(Luan et al., 2006)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Blocking synaptic vesicle exocytosis (using Ctet\TeTxLC^TNT.Scer\UAS) in the split-GAL4 combination of Scer\GAL4^dsx-GAL4-DBD and Hsim\VP16^{AD.elav.T:Zzzz\Zip+,T:SV40\nls-largeT} neurons in males and females results in a significant drop (compared to genetic controls) in courtship of females by males, males completely fail to copulate and are behaviorally sterile; females are sterile, unreceptive and show no post-mating behavioral responses.

(Pavlou et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

23872

w^*; P{UAS-2xEGFP}AH2; P{elav-VP16.AD}G3A1

81295

w¹¹¹⁸; P{nSyb-QF.DBD}attP40; P{elav-VP16.AD}G3A1

Notes on Origin

Discoverer

Comments

The T:Disc\RFP tag is not expressed from this construct.

(Luan et al., 2006)

When Hsim\VP16^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2} and Scer\GAL4^{DBD.elav.T:Zzzz\ZipRREEL} are expressed together in the same cell, they function as a complete GAL4 driver.

(Luan et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Hsim\VP16^{AD.elav.T:Zzzz\Zip+,T:SV40\nls-largeT}

Hsim\VP16^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2}

Hsim\VP16^AD.elav

elav^VP16AD

(Luan et al., 2006, Luan et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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