Open Close
General Information
Symbol
Hsim\VP16AD.elav
Species
S. Herpes simplex virus Type 1
Name
FlyBase ID
FBal0198078
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
elav-VP16AD
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

An elav promoter regulates expression of a VP16(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the VP16 activation domain). The hemidriver sequence includes the Tag:NLS(SV40-largeT) nuclear localization signal. An internal ribosomal entry site (IRES) from the Ubx gene followed by DsRed(Unk) coding sequence is present downstream of the hemidriver sequence, but this is not expressed in the nervous system.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Hsim\VP16AD.elav drives expression specifically in neurons when combined with Scer\GAL4dsx-GAL4-DBD in a split-GAL4 system including mechanosensory neurons in the terminalia associated with the bristles of the clasper tooth, lateral plates and anal plates in males, and the anal and vaginal plates in females.

Hsim\VP16AD.elav drives expression pan-neuronally, recapitulating the elav expression pattern, when combined with Scer\GAL4DBD.elav in a split-GAL4 system.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Blocking synaptic vesicle exocytosis (using Ctet\TeTxLCTNT.Scer\UAS) in the split-GAL4 combination of Scer\GAL4dsx-GAL4-DBD and Hsim\VP16AD.elav.T:Zzzz\Zip+,T:SV40\nls-largeT neurons in males and females results in a significant drop (compared to genetic controls) in courtship of females by males, males completely fail to copulate and are behaviorally sterile; females are sterile, unreceptive and show no post-mating behavioral responses.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

The T:Disc\RFP tag is not expressed from this construct.

When Hsim\VP16AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2 and Scer\GAL4DBD.elav.T:Zzzz\ZipRREEL are expressed together in the same cell, they function as a complete GAL4 driver.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Hsim\VP16AD.elav.T:Zzzz\Zip+,T:SV40\nls-largeT
Hsim\VP16AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2
Hsim\VP16AD.elav
Name Synonyms
Secondary FlyBase IDs
    References (10)