FlyBase Allele Report: Dmel\γTub23C[GD8749]

General Information

Symbol

Dmel\γTub23C^GD8749

Species

D. melanogaster

Name

FlyBase ID

FBal0198572

Feature type

allele

Associated gene

Dmel\γTub23C

Associated Insertion(s)

Carried in Construct

P{GD8749}

Also Known As

γTub23C RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8749}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8749

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

dendrite | larval stage | conditional, with Scer\GAL4²²¹

(Chen et al., 2012)

larval dorsal multidendritic neuron ddaE | larval stage | conditional, with Scer\GAL4²²¹

(Chen et al., 2012)

microtubule | larval stage | conditional, with Scer\GAL4²²¹

(Chen et al., 2012)

Detailed Description

Statement

Reference

Larval ddaE neurons expressing γTub23C^GD8749 under the control of Scer\GAL4²²¹ (and Dicer-2, for a more efficient RNAi) show a significant decrease in growing microtubules within the cell body 24h after axotomy, but do not show changes in axon regeneration at 48h upon proximal axotomy, as compared to similarly injured control neurons.

Larval ddaC neurons expressing γTub23C^GD8749 under the control of Scer\GAL4⁴⁷⁷ (and Dicer-2, for a more efficient RNAi) show the expected complex dendrite arbors that increases in branching during larval life.

Expression of γTub23C^GD8749 under the control of Scer\GAL4^GMR.long does not induce any visible eye defect compared to controls.

(Chen et al., 2012)

Adults expressing γTub23C^GD8749 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development