Scer\GAL4Cg.PA SPARCGD5660 larvae fail to pupate, with some arresting as 3rd instars for up to 10 days. In contrast to wild type, only about half of these mutant larvae are feeding at any one time.
Fat body from Scer\GAL4Cg.PA SPARCGD5660 3rd instar larvae shows a mild disruption of tissue morphology - adipocytes are rounded and membranes bleb. Whereas wild type adipocytes are polygonal, these mutant adipocytes are spherical with an increased number of surface pits. TEM shows that these cells have abnormal ribosome-enriched cytoplasm with a dramatic accumulation of fibrous ECM at cell boundaries - the basal lamina juxtaposed to the adipocytes is fibrous and highly variable in thickness.
Adults expressing SPARCGD5660 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in early larval lethality.
Expression under the control of Scer\GAL4Mef2.PR results in grossly normal larval body wall muscles.
Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.