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General Information
Symbol
Dmel\SPARCGD5660
Species
D. melanogaster
Name
FlyBase ID
FBal0198818
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4Cg.PA SPARCGD5660 larvae fail to pupate, with some arresting as 3rd instars for up to 10 days. In contrast to wild type, only about half of these mutant larvae are feeding at any one time.

Fat body from Scer\GAL4Cg.PA SPARCGD5660 3rd instar larvae shows a mild disruption of tissue morphology - adipocytes are rounded and membranes bleb. Whereas wild type adipocytes are polygonal, these mutant adipocytes are spherical with an increased number of surface pits. TEM shows that these cells have abnormal ribosome-enriched cytoplasm with a dramatic accumulation of fibrous ECM at cell boundaries - the basal lamina juxtaposed to the adipocytes is fibrous and highly variable in thickness.

Adults expressing SPARCGD5660 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Clonal activation of BM-40-SPARCGD5660 using Scer\GAL4αTub84B.PP does not affect the survival of the BM-40-SPARCGD5660-expressing cells surrounded by wild-type cells.

Expression under the control of Scer\GAL4Mef2.PR results in early larval lethality.

Expression under the control of Scer\GAL4Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

When cell clones expressing Scer\GAL4αTub84B.PBb>BM-40-SPARCGD5660 are surrounded by dmαTub84B.PBb-expressing cells in the super-competitor assay, the majority of BM-40-SPARCGD5660-expressing cells are eliminated, and the clones are strongly reduced in size at 48 h after clone induction (ACI), whereas loser cells not expressing BM-40-SPARCGD5660 show this effect only at 72 h ACI.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
BM-40-SPARCGD5660
SPARCGD5660
Name Synonyms
Secondary FlyBase IDs
    References (11)