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General Information
Symbol
Dmel\SPRGD3236
Species
D. melanogaster
Name
FlyBase ID
FBal0201616
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-SPR-IR1, UAS-SPR-IR
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of SPRGD3236 under the control of Scer\GAL4elav.PLu (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in a significant decrease in egg-laying without affecting female receptivity, as compared to controls.

Virgin females expressing SPRGD3236 under the control of Scer\GAL4dsx.KI are typically receptive. After mating they show no decrease in sexual receptivity or increase in ovipositor extrusions, in contrast to that seen in mated control females. Mated females expressing SPRGD3236 under the control of Scer\GAL4dsx.KI show locomotor activity comparable to that of virgin control females and have significantly decreased levels of oviposition compared to mated control females.

Males spend significantly more time courting mated females expressing SPRGD3236 under the control of Scer\GAL4dsx.KI compared to the time they spend courting control mated females. This effect is not seen if the males are paired with mutant decapitated females.

Expression of SPRGD3236 under the control of Scer\GAL4elav.PLu, Scer\GAL4ppk.PG, Scer\GAL4NP2024, Scer\GAL4UH3-50, Scer\GAL45-136, Scer\GAL4UH11-2, Scer\GAL4UH14-15 or Scer\GAL4UH21-49 significantly reduces egg laying and increases mating receptivity in mated females, with no effect on virgin female mating receptivity, compared to controls.

Expression of SPRGD3236 under the control of Scer\GAL4ppk.PG in sex peptide-injected virgin females increases mating receptivity, compared to controls.

Virgin females expressing SPRGD3236 under the control of Scer\GAL4ppk.PG have normal sexual receptivity and a low egg-laying rate. However, mated females expressing SPRGD3236 under the control of Scer\GAL4ppk.PG do not show a reduction in receptivity to courting males and do not increase their egg-laying rate, in contrast to wild-type mated females.

Mated females expressing SPRGD3236 under the control of Scer\GAL4fru.P1.D do not show the normal postmating behaviours of a strongly reduced sexual receptivity and an increase in egg-laying rate. These postmating behaviours are restored if the mated females also carry Scer\GAL80ppk.PY.

Virgin females expressing SPRGD3236 under the control of Scer\GAL4elav.PLu show the same receptivity towards a naive wild-type male as control females. However, these females lay very few eggs after mating, in contrast to control females. If the mutant females are then tested for receptivity towards a second naive male after being allowed to lay eggs for 48 hours, they mate again at a high frequency and do not actively reject the second male, in contrast to control females tested under the same conditions.

Virgin females expressing SPRGD3236 under the control of Scer\GAL4elav.PLu remain receptive in pairings with wild-type males even after injection of Acp70A protein, in contrast to wild-type females which are unreceptive after this treatment.

Virgin females expressing SPRGD3236 under the control of Scer\GAL4fru-GAL4 (these females also carry Dcr-2Scer\UAS.cDa to enhance the transgenic RNAi effect) show the same receptivity towards a naive wild-type male as control females. However, these females lay very few eggs after mating, in contrast to control females. If the mutant females are then tested for receptivity towards a second naive male after being allowed to lay eggs for 48 hours, they mate again at a high frequency, in contrast to control females tested under the same conditions

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CG16752GD3236
SPRGD3236
Name Synonyms
Secondary FlyBase IDs
    References (11)