Expression of Eato^GD1133 under the control of Scer\GAL4^GR1 results in nurse cell clearance defect in mutant stage 14 egg chambers that contain persistent nurse cell nuclei, while in wild-type egg chambers all the germ-line derived nurse cells have undergone programmed cell death and been cleared by this stage. Scer\GAL4^{nos.UTR.T:Hsim\VP16}-driven expression of Eato^GD1133 does not lead to clearance defects.

Similarly, Scer\GAL4^GR1>Eato^GD1133 mutants exhibit defects in engulfment of nurse cells by the follicle cells during starvation-induced programmed cell death: While phase 0 and phase 1 egg chambers resemble the control, in phase 3 eggs their follicle cells show fewer engulfed Dcp-1-positive vesicles and by phase 5 the dying mutant egg chambers display severe engulfment defects and follicle cell death, leaving behind completely unengulfed germline. No obvious engulfment defects in dying egg chambers are observed if Eato^GD1133 is controlled by Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

Adults expressing CG31731^GD1133 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.