Under the control of Scer\GAL4Orco.PU, expression of GABA-B-R2GD699 leads to the elimination of the starvation-dependent increase in attraction to pentyl acetate compared to control larvae. More dramatically, under the control of Scer\GAL4Or47a.PU, it leads to the repulsion of the starved larvae by the odor; while it leads to the attraction of the non-starved larvae to the odor at similar strengths compared to controls.
Under the control of Scer\GAL4Orco.PU, expression of GABA-B-R2GD699 leads to a significant increase in body weight although it does not alter the levels of food intake relative to control larvae. Under the control of Scer\GAL4Or47a.PU, it leads to a lower levels of food intake relative to control larvae.
Expression of GABA-B-R2GD699 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4GMR41E11 results in defects in sensing food hardness as the flies show diminished preference for softer food to harder food at the expense of sweetness, while the preference for sweeter food when hardness is identical remains unperturbed.
RNAi using GABA-B-R2GD699 in neurons targeted by the Split-GAL4 combination of Scer\GAL4dsx-GAL4-DBD and Hsim\VP16AD.VGlut.OK371.T:Zzzz\Zip+,T:SV40\nls-largeT results in a significant defect in copulation termination in males (around 15% of males display a 'stuck' phenotype, an inability to uncouple from the female) compared to genetic controls; there are no significant differences in the percentage of successful copulations or male fertility or copulation duration.
Expression of GABA-B-R2GD699 under the control of Scer\GAL4dimm-929 does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.
Adults expressing GABA-B-R2GD699 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.