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General Information
Symbol
Dmel\GABA-B-R2GD699
Species
D. melanogaster
Name
FlyBase ID
FBal0207069
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-GABABR2-RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Under the control of Scer\GAL4Orco.PU, expression of GABA-B-R2GD699 leads to the elimination of the starvation-dependent increase in attraction to pentyl acetate compared to control larvae. More dramatically, under the control of Scer\GAL4Or47a.PU, it leads to the repulsion of the starved larvae by the odor; while it leads to the attraction of the non-starved larvae to the odor at similar strengths compared to controls.

Under the control of Scer\GAL4Orco.PU, expression of GABA-B-R2GD699 leads to a significant increase in body weight although it does not alter the levels of food intake relative to control larvae. Under the control of Scer\GAL4Or47a.PU, it leads to a lower levels of food intake relative to control larvae.

Expression of GABA-B-R2GD699 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4GMR41E11 results in defects in sensing food hardness as the flies show diminished preference for softer food to harder food at the expense of sweetness, while the preference for sweeter food when hardness is identical remains unperturbed.

RNAi using GABA-B-R2GD699 in neurons targeted by the Split-GAL4 combination of Scer\GAL4dsx-GAL4-DBD and Hsim\VP16AD.VGlut.OK371.T:Zzzz\Zip+,T:SV40\nls-largeT results in a significant defect in copulation termination in males (around 15% of males display a 'stuck' phenotype, an inability to uncouple from the female) compared to genetic controls; there are no significant differences in the percentage of successful copulations or male fertility or copulation duration.

Expression of GABA-B-R2GD699 under the control of Scer\GAL4dimm-929 does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

Flies expressing GABA-B-R2GD699 in astrocytes under the control of Scer\GAL4alrm.PD show similar recovery times to mechanical stress as in wild type.

Flies expressing GABA-B-R2GD699 in the LN[[v]]s under the control of Scer\GAL4P0.5.Pdf exhibit longer periods than controls (24.3hr).

Adults expressing GABA-B-R2GD699 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of GABA-B-R2GD699 in astrocytes under the control of Scer\GAL4alrm.PD partially suppresses the delayed recovery seen in eas2 mutant flies in response to mechanical shock.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
GABA-B-R2GD699
Name Synonyms
Secondary FlyBase IDs
    References (14)