FlyBase Allele Report: Dmel\GABA-B-R2[GD699]

General Information

Symbol

Dmel\GABA-B-R2^GD699

Species

D. melanogaster

Name

FlyBase ID

FBal0207069

Feature type

allele

Associated gene

Dmel\GABA-B-R2

Associated Insertion(s)

Carried in Construct

P{GD699}

Also Known As

UAS-GABA_BR2-RNAi

Key Links

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Dickson et al., 2007.7.18)

in vitro construct - regulatory fusion

(Dickson et al., 2007.7.18)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GD699}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

GABA-B-R2

Associated Sequence Features

dsRNA-GD699

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensitive behavior | third instar larval stage, with Scer\GAL4^Orco.PU

(Slankster et al., 2020)

abnormal chemosensitive behavior | third instar larval stage | calorie restriction conditional, with Scer\GAL4^Or47a.PU

(Slankster et al., 2020)

abnormal circadian rhythm, with Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

abnormal copulation | male, with Hsim\VP16^{AD-VGlut-OK371}, Scer\GAL4^dsx-GAL4-DBD

(Pavlou et al., 2016)

abnormal feeding behavior | third instar larval stage, with Scer\GAL4^Or47a.PU

(Slankster et al., 2020)

abnormal touch perception | adult stage, with Scer\GAL4^GMR41E11

(Jeong et al., 2016)

increased size | adult stage, with Scer\GAL4^nub.PU

(Saad and Hipfner, 2021)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^nub.PU

(Saad and Hipfner, 2021)

Phenotype Manifest In

wing, with Scer\GAL4^nub.PU

(Saad and Hipfner, 2021)

wing vein | ectopic, with Scer\GAL4^nub.PU

(Saad and Hipfner, 2021)

Detailed Description

Statement

Reference

Under the control of Scer\GAL4^Orco.PU, expression of GABA-B-R2^GD699 leads to the elimination of the starvation-dependent increase in attraction to pentyl acetate compared to control larvae. More dramatically, under the control of Scer\GAL4^Or47a.PU, it leads to the repulsion of the starved larvae by the odor; while it leads to the attraction of the non-starved larvae to the odor at similar strengths compared to controls.

Under the control of Scer\GAL4^Orco.PU, expression of GABA-B-R2^GD699 leads to a significant increase in body weight although it does not alter the levels of food intake relative to control larvae. Under the control of Scer\GAL4^Or47a.PU, it leads to a lower levels of food intake relative to control larvae.

(Slankster et al., 2020)

Expression of GABA-B-R2^GD699 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^GMR41E11 results in defects in sensing food hardness as the flies show diminished preference for softer food to harder food at the expense of sweetness, while the preference for sweeter food when hardness is identical remains unperturbed.

(Jeong et al., 2016)

RNAi using GABA-B-R2^GD699 in neurons targeted by the Split-GAL4 combination of Scer\GAL4^dsx-GAL4-DBD and Hsim\VP16^{AD.VGlut.OK371.T:Zzzz\Zip+,T:SV40\nls-largeT} results in a significant defect in copulation termination in males (around 15% of males display a 'stuck' phenotype, an inability to uncouple from the female) compared to genetic controls; there are no significant differences in the percentage of successful copulations or male fertility or copulation duration.

(Pavlou et al., 2016)

Expression of GABA-B-R2^GD699 under the control of Scer\GAL4^dimm-929 does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Flies expressing GABA-B-R2^GD699 in astrocytes under the control of Scer\GAL4^alrm.PD show similar recovery times to mechanical stress as in wild type.

(Muthukumar et al., 2014)

Flies expressing GABA-B-R2^GD699 in the LN[[v]]s under the control of Scer\GAL4^P0.5.Pdf exhibit longer periods than controls (24.3hr).

(Dahdal et al., 2010)

Adults expressing GABA-B-R2^GD699 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

GABA-B-R2^GD699, Scer\GAL4^repo is a non-enhancer of abnormal wound healing | larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo, btl::Egfr^λ.UAS

(Li et al., 2020)

Suppressor of

Statement

Reference

GABA-B-R2^GD699/Scer\GAL4^ppk.PU is a suppressor | partially of abnormal wound healing | larval stage phenotype of Myc^QUAS.cPa, Ncra\QF^repo

(Li et al., 2020)

GABA-B-R2^GD699/Scer\GAL4^alrm.PD is a suppressor | partially of abnormal stress response phenotype of eas²

(Muthukumar et al., 2014)

NOT Suppressor of

Statement

Reference

GABA-B-R2^GD699, Scer\GAL4^repo is a non-suppressor of abnormal wound healing | larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo, btl::Egfr^λ.UAS

(Li et al., 2020)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

GABA-B-R2^GD699, Scer\GAL4^repo is a non-enhancer of larval multidendritic class IV neuron phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo, btl::Egfr^λ.UAS

(Li et al., 2020)

Suppressor of

Statement

Reference

GABA-B-R2^GD699/Scer\GAL4^ppk.PU is a suppressor | partially of larval multidendritic class IV neuron phenotype of Myc^QUAS.cPa, Ncra\QF^repo

(Li et al., 2020)

NOT Suppressor of

Statement

Reference

GABA-B-R2^GD699, Scer\GAL4^repo is a non-suppressor of larval multidendritic class IV neuron phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo, btl::Egfr^λ.UAS

(Li et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of GABA-B-R2^GD699 in astrocytes under the control of Scer\GAL4^alrm.PD partially suppresses the delayed recovery seen in eas² mutant flies in response to mechanical shock.

(Muthukumar et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v1784

w¹¹¹⁸; P{GD699}v1784

v1785

w¹¹¹⁸; P{GD699}v1785

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Crossreferences

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GABA-B-R2^GD699

GABA_BR2^GD

(Jeong et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (16)

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