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FB2021_02, released April 13, 2021

Allele: Dmel\GABA-B-R2GD699

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General Information
Symbol
Dmel\GABA-B-R2GD699
Species
D. melanogaster
Name
FlyBase ID
FBal0207069
Feature type
allele
Associated gene
Dmel\GABA-B-R2
Associated Insertion(s)
Carried in Construct
P{GD699}
Also Known As
UAS-GABABR2-RNAi
Key Links
Genomic Maps
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - RNAi
(Dickson et al., 2007.7.18)
in vitro construct - regulatory fusion
(Dickson et al., 2007.7.18)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{GD699}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt regulatory sequences drive expression of an inverted repeat.

      (Dickson et al., 2007.7.18)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      GABA-B-R2
      Associated Sequence Features
      dsRNA-GD699
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Under the control of Scer\GAL4Orco.PU, expression of GABA-B-R2GD699 leads to the elimination of the starvation-dependent increase in attraction to pentyl acetate compared to control larvae. More dramatically, under the control of Scer\GAL4Or47a.PU, it leads to the repulsion of the starved larvae by the odor; while it leads to the attraction of the non-starved larvae to the odor at similar strengths compared to controls.

      Under the control of Scer\GAL4Orco.PU, expression of GABA-B-R2GD699 leads to a significant increase in body weight although it does not alter the levels of food intake relative to control larvae. Under the control of Scer\GAL4Or47a.PU, it leads to a lower levels of food intake relative to control larvae.

      (Slankster et al., 2020)

      Expression of GABA-B-R2GD699 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4GMR41E11 results in defects in sensing food hardness as the flies show diminished preference for softer food to harder food at the expense of sweetness, while the preference for sweeter food when hardness is identical remains unperturbed.

      (Jeong et al., 2016)

      RNAi using GABA-B-R2GD699 in neurons targeted by the Split-GAL4 combination of Scer\GAL4dsx-GAL4-DBD and Hsim\VP16AD.VGlut.OK371.T:Zzzz\Zip+,T:SV40\nls-largeT results in a significant defect in copulation termination in males (around 15% of males display a 'stuck' phenotype, an inability to uncouple from the female) compared to genetic controls; there are no significant differences in the percentage of successful copulations or male fertility or copulation duration.

      (Pavlou et al., 2016)

      Expression of GABA-B-R2GD699 under the control of Scer\GAL4dimm-929 does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

      (Shankar et al., 2015)

      Flies expressing GABA-B-R2GD699 in astrocytes under the control of Scer\GAL4alrm.PD show similar recovery times to mechanical stress as in wild type.

      (Muthukumar et al., 2014)

      Flies expressing GABA-B-R2GD699 in the LN[[v]]s under the control of Scer\GAL4P0.5.Pdf exhibit longer periods than controls (24.3hr).

      (Dahdal et al., 2010)

      Adults expressing GABA-B-R2GD699 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of GABA-B-R2GD699 in astrocytes under the control of Scer\GAL4alrm.PD partially suppresses the delayed recovery seen in eas2 mutant flies in response to mechanical shock.

      (Muthukumar et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Crossreferences
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      GABA-B-R2GD699
      GABABR2GD
      (Jeong et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (14)