FlyBase Allele Report: Dmel\Inx6[GD3692]

General Information

Symbol

Dmel\Inx6^GD3692

Species

D. melanogaster

Name

FlyBase ID

FBal0209738

Feature type

allele

Associated gene

Dmel\Inx6

Associated Insertion(s)

Carried in Construct

P{GD3692}

Also Known As

inx6 RNAi

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Dickson et al., 2007.7.18)

in vitro construct - regulatory fusion

(Dickson et al., 2007.7.18)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GD3692}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Inx6

Associated Sequence Features

dsRNA-GD3692

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^GMR23E10

(Troup et al., 2018)

abnormal memory, with Scer\GAL4^GH146, Scer\GAL4^c316

(Wu et al., 2011)

abnormal memory | adult stage, with Hsap\RELA^AD.R12C11, Scer\GAL4^DBD.R14C08

(Liu et al., 2016)

abnormal memory | adult stage, with Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R41C07

(Liu et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^17d

(Liu et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^Mef2.247

(Liu et al., 2016)

abnormal memory | adult stage | RU486 conditional, with Scer\GAL4^{Mef2.247.Switch}

(Liu et al., 2016)

abnormal sleep | adult stage, with Scer\GAL4^GMR23E10

(Troup et al., 2018)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

(Tsubouchi et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Inx6^GD3692 under the control of Scer\GAL4^GMR23E10 in adults significantly decreases daytime and nighttime sleep duration, and sleep intensity, and significantly increases responsiveness to disturbance, compared to controls; when expression is restricted, using a Gal80[ts] transgene, to the 24 h prior to 48 h testing, daytime, but not nighttime, responsiveness is significantly increased, and daytime, but not nighttime, sleep intensity is significantly decreased, with no effect on sleep duration, compared to controls.

(Troup et al., 2018)

Expression of Inx6^GD3692 under the control of Scer\GAL4^17d, the split-GAL4 pair of Scer\GAL4^DBD.R41C07 and Hsap\RELA^AD.R13F02, the split-GAL4 pair of Scer\GAL4^DBD.R14C08 and Hsap\RELA^AD.R12C11, or Scer\GAL4^Mef2.247, but not the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02, significantly impairs visual short term memory following training with pain stimulus in adult flies, compared to controls; this effect is also seen when Inx6^GD3692 is expressed during the adult stage only, under the control of Scer\GAL4^{Mef2.247.Switch} (induced by RU486 application for three days prior to the experiment); no memory defect is seen using Scer\GAL4^17d when expression is restricted to a smaller subset of cells using Scer\GAL80^Mef2.mb247.

(Liu et al., 2016)

Expression of inx6^GD3692 in both the anterior paired lateral (APL) and dorsal paired medial (DPM) neurons in the mushroom body (using the Scer\GAL4^GH146 and Scer\GAL4^c316 drivers simultaneously, and in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) results in impaired 3 hour (intermediate-term) memory after associative conditioning compared to controls.

(Wu et al., 2011)

Adults expressing Inx6^GD3692 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3692

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Inx6^GD3692, Scer\GAL4^GMR23E10 has abnormal sleep | adult stage phenotype, suppressible | conditional | partially by Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Suppressor of

Statement

Reference

Inx6^GD3692, Scer\GAL4^GMR23E10 is a suppressor | partially of abnormal sleep | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Inx6^GD3692, Scer\GAL4^GMR23E10 is a suppressor | partially of abnormal locomotor behavior | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Inx6^GD3692, Scer\GAL4^GMR23E10 is a suppressor | partially of abnormal neurophysiology | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Phenotype Manifest In

Suppressor of

Statement

Reference

Inx6^GD3692, Scer\GAL4^GMR23E10 is a suppressor | partially of fan-shaped body | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Inx6^GD3692 under the control of Scer\GAL4^GMR23E10 blocks the secondary spikes and local field potential oscillations, but not the increased membrane potential, induced by acute optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with (red) light) of Scer\GAL4^GMR23E10 cells in adults; this expression also suppresses the sleep duration and sleep intensity increases and the responsiveness decrease caused by prolonged optogenetic activation.

Prolonged optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with (red) light) of Scer\GAL4^GMR23E10 cells in adults suppresses the sleep duration decrease, but not the responsiveness increase or the sleep intensity decrease caused by expression of Inx6^GD3692 under the control of Scer\GAL4^GMR23E10.

(Troup et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v8639

w¹¹¹⁸ P{GD3692}v8639

v8638

w¹¹¹⁸; P{GD3692}v8638