FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Cul1GD9650
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General Information
Symbol
Dmel\Cul1GD9650
Species
D. melanogaster
Name
FlyBase ID
FBal0209934
Feature type
allele
Associated gene
Dmel\Cul1
Associated Insertion(s)
Carried in Construct
P{GD9650}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD9650}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD9650
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adults expressing Cul1GD9650 under the control of Scer\GAL4GMR.PU do not present any obvious eye defects.

      (Chen et al., 2019)

      Scer\GAL4sd.PU-mediated expression of Cul1GD9650 results in rudimentary wings showing morphological features of the notum.

      Scer\GAL4sd.PU-mediated expression of Cul1GD9650 induces cell death in wing discs.

      (Fereres et al., 2013)

      Expression of lin19GD9650 under the control of two copies of Scer\GAL4ppk.PG produces severe dendrite pruning defects in ddaC neurons at 16 hours after puparium formation. No difference is seen at the white prepupal stage.

      (Wong et al., 2013)

      Flies expressing lin19GD9650 under the control of Scer\GAL4GMR.PU have a slight rough eye phenotype.

      (Bhutani et al., 2012)

      Adults expressing Cul1GD9650 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver can show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies, depending on the particular P{GD9650} insertion line used.

      (Neely et al., 2010)

      Adults expressing Cul1GD9650 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies, early pupal lethality or late larval lethality.

      Expression under the control of Scer\GAL4Mef2.PR results in grossly normal larval body wall muscles.

      Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

      (Schnorrer et al., 2010)

      Expression under the control of Scer\GAL4pnr-MD237 may result in lethality, depending on the insertion line used.

      (Mummery-Widmer et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Other
      Phenotype Manifest In
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      Cul1GD9650, Scer\GAL4ppk.PG has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by PtenUAS.cHa, Scer\GAL4ppk.PG

      (Wong et al., 2013)

      Cul1GD9650, Scer\GAL4ppk.PG has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by gigUAS.cTa/Tsc1UAS.cPa, Scer\GAL4ppk.PG

      (Wong et al., 2013)

      Cul1GD9650, Scer\GAL4ppk.PG has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by mTorTED.UAS, Scer\GAL4ppk.PG

      (Wong et al., 2013)

      Cul1GD9650, Scer\GAL4ppk.PG has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by ThorT37A.T46A.UAS, Scer\GAL4ppk.PG

      (Wong et al., 2013)

      Cul1GD9650, Scer\GAL4ppk.PG has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by S6kKQ.UAS, Scer\GAL4ppk.PG

      (Wong et al., 2013)

      Cul1GD9650, Scer\GAL4ppk.PG has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible by AktHM04007, Scer\GAL4ppk.PG

      (Wong et al., 2013)

      Cul1GD9650, Scer\GAL4ppk.PG has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible by AktHMS00007, Scer\GAL4ppk.PG

      (Wong et al., 2013)

      Cul1GD9650, Scer\GAL4ppk.PG has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by InRK1409A.UAS, Scer\GAL4ppk.PG

      (Wong et al., 2013)

      Cul1GD9650, Scer\GAL4ppk.PG has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by Pi3K92ED954A.UAS.Tag:MYC, Scer\GAL4ppk.PG

      (Wong et al., 2013)
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL4sd.PU-mediated expression of both Cul1GD9650 and RYBPdsRNA.Scer\UAS.cGa results in flies completely lacking wing tissue.

      (Fereres et al., 2013)

      Expression of ciCe-2.Scer\UAS.T:Ivir\HA1 does not suppress the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of MicalN-ter.Scer\UAS does not suppress the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of sggS9A.Scer\UAS does not suppress the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of InRK1409A.Scer\UAS dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of NECN.Scer\UAS.cGa does not suppress the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation. No difference is seen in white prepupae.

      Expression of PtenScer\UAS.cHa dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of Tsc1Scer\UAS.cPa and gigScer\UAS.cTa suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of TorTED.Scer\UAS suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of ThorT37A.T46A.Scer\UAS dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of S6kKQ.Scer\UAS dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of Akt1HM04007 dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      Expression of Akt1HMS00007 dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      (Wong et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference

      The eye defects induced by the expression of Hsap\ATXN3tr.Q78.UAS.Tag:HA under the control of Scer\GAL4GMR.PU are aggravated by the co-expression of either Cul1GD9650 or FipoQNIG.2010R to the point of even leading to necrotic scars.

      Adults co-expressing Hsap\ATXN3tr.Q27.UAS.Tag:HA and Cul1GD9650 under the control of Scer\GAL4GMR.PU do not present any obvious eye defects.

      Adults co-expressing Hsap\ATXN3fl.Q84.UAS.Tag:MYC and Cul1GD9650 under the control of Scer\GAL4GMR.PU display mild eye depigmentation; this phenotype is not enhanced by the additional expression of FipoQNIG.2010R.

      (Chen et al., 2019)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Cul1GD9650
      lin19GD9650
      Name Synonyms
      Secondary FlyBase IDs
        References (11)