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General Information
Symbol
Dmel\Cad99C21-5
Species
D. melanogaster
Name
FlyBase ID
FBal0212148
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{EP}Cad99CGE21034 results in a large deletion covering the 5'UTR and exons 1-7, and part of exon 8 of Cad99C.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Cad99C21-5 mutant females lay ~50% fewer eggs than wild-type females, with <2% of those eggs producing larvae. These larvae develop into flies that only display defects if they are homozygous mutants. The majority of eggs from Cad99C21-5 mutant females collapse soon after deposition and even non-collapsed eggs are penetrable to vital dyes (e.g. trypan blue), which do not stain wild-type eggs. These defects suggest that eggshells, which normally restrict permeability and prevent desiccation, are compromised. Eggs from mutant females are also highly intolerant to sodium hydrochlorite. Hydrochlorite treatment of wild-type eggs removes the outer eggshell, the chorion, but leaves the inner eggshell, the vitelline membrane, intact. Disintegration of eggs from Cad99C21-5 mutant females in hydrochlorite suggests that the vitelline membrane is non-functional.

The vitelline membrane of Cad99C21-5 mutant follicles varies in thickness and contains numerous holes. This variability in eggshell defects may explain why mutant females are not fully sterile, allow a few embryos to develop.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Cad99CScer\UAS.fl in follicle cells under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C rescues microvillus formation in a Cad99C21-5/Cad99C21-6 mutant background.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)