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General Information
Symbol
Dmel\Eps-15e75
Species
D. melanogaster
Name
FlyBase ID
FBal0219694
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
eps15e75
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the insertion in Eps-15EP2513. Remnants of the inserted element are still present at the original insertion site.

Insertion components
P{?EP}Eps-15e75
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
exacerbates  Troyer syndrome
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The neuromuscular junctions of Eps-15e75/Eps-15Δ29 third instar larvae show impaired FM1-43FX dye uptake compared to wild type, indicating a defect in synaptic vesicle endocytosis.

The average number of boutons per neuromuscular junction (NMJ) is increased in Eps-15e75/Eps-15Δ29 mutant larvae. In addition, the mutant NMJs have more boutons with two branches and more boutons with three or more branches than controls.

Spontaneous miniature excitatory junctional potentials (mEJPs) at the NMJ of Eps-15e75/Eps-15Δ29 larvae have a similar amplitude, but higher frequency, than those of controls.

When the NMJ of Eps-15e75/Eps-15Δ29 or Eps-15e75/Df(2R)Dll-MP larvae is stimulated at a low frequency, evoked EJPs have a similar amplitude to those of controls. However, under high-frequency stimulation conditions (10Hz for 10 minutes), the mutant NMJs show strong synaptic depression to approximately 40% of the initial EJP amplitude (in contrast to control NMJs, which are able to maintain EJPs at approximately 90% of the initial amplitude).

Eps-15e75/Eps-15Δ29 third instar larvae show severely reduced dye uptake at the neuromuscular junction during nerve stimulation compared to controls, indicating a defect in endocytosis.

The mean vesicle density in Eps-15e75/Eps-15Δ29 larval boutons at rest is not significantly different from that of controls, although there is an increased proportion of abnormally large vesicles or cisternae in the resting mutant boutons. During a 90mM K[+] stimulation, the mutant boutons show a reduction in mean vesicle density compared to controls and there is an increased proportion of large cisternae that are greater than 85nm in size (some of these cisternae appear to be large membrane invaginations which are contiguous with the plasma membrane). When the mutant boutons are allowed to recover for 1 minute after stimulation, the mean vesicle density shows a poor recovery, being reduced compared to resting levels (in contrast to wild-type boutons which recover the vesicle density to resting levels). The size distribution of boutons after a 1 minute recovery from stimulation is normal in the mutants.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement
Reference

Eps-15e75 is a suppressor | partially of wing vein L5 phenotype of rhove-1

Additional Comments
Genetic Interactions
Statement
Reference

Eps-15e75/+ ; spartin1/+ double heterozygotes show impaired FM1-43FX dye uptake at the neuromuscular junction compared to wild type.

An Eps-15e75 heterozygous background leads to approximately 70% suppression of the L5 wing vein defect in both rhove-1/Df(3L)ru-22 kst1 and rhove-1/rhove-1 kst1 flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Separable from: a second site lethal mutation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (5)