FB2026_02 , released June 18, 2026
Allele: Dmel\Ir25a2
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General Information
Symbol
Dmel\Ir25a2
Species
D. melanogaster
Name
FlyBase ID
FBal0220383
Feature type
allele
Associated gene
Dmel\Ir25a
Associated Insertion(s)
TI{TI}Ir25a2
Carried in Construct
Key Links
Allele class

loss of function allele

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Abuin et al., 2011)

loss of function allele

(Benton et al., 2009)
Mutagen
recombination
(Benton et al., 2009)
gene targeting by homologous recombination
(Benton et al., 2009)
Progenitor genotype
Associated Insertion(s)
TI{TI}Ir25a2
Cytology
Description
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ir25a2 mutant flies do not show significant changes in the expected avoidance response to food laced with different acids (5% acetic acid, 5% glycolic acid, 10% citric acid, 20% lactic acid, 5% tartaric acid, 2% propionic acid, 2% butyric acid, or 10mM HCl), as compared to controls. GRNs enervating S6 and S10 sensilla do not show changes in the responses (frequency of spikes) to acetic acid (1%), glycolic acid (1%), tartaric acid (1%), citric acid (5%), propionic acid (1%), butyric acid (1%), HCl (10mM), and lactic acid (20%), as compared to controls.

      (Rimal et al., 2019)

      Ir25a2/Ir25a2 adult females have significantly reduced preference for oviposition on acid-containing medium, compared to controls.

      (Chen and Amrein, 2017)

      Ir25a2/Ir25a2 mutant females do not exhibit a significant reduction in feeding preference index for 1% yeast extract versus 5mM sucrose solution, as compared to wild type.

      (Ganguly et al., 2017)

      Ir25a2 homozygous mutant larvae do not have any alteration in amino acid preference, compared to controls.

      (Croset et al., 2016)

      In olfactory preference assays for polyamines, Ir25a2/Ir25a2 flies do not show any significant difference in their attraction to putrescine or cadaverine, as compared to wild type, and females do not show any significant difference in their aversion to oviposit on polyamine-rich substrate (plain, sugar-free 1% agarose supplemented with either putrescine or cadaverine) versus non-supplemented substrate, as compared to wild type.

      (Hussain et al., 2016)

      Ir25a2 mutant larvae have significantly decreased bitter avoidance, compared to controls.

      (van Giesen et al., 2016)

      Ir25a2 homozygous adults are able to synchronize circadian locomotor activity to high amplitude (4[o]C) temperature cycles under constant light (LL) or constant dark (DD) conditions, similar to controls; at a range of smaller (2[o]C) temperature intervals, Ir25a2 adults fail to synchronize to temperature cycles under LL; they also have a significantly reduced phase-shift in circadian locomotor activity, compared to controls, when shallow (2[o]C) temperature cycles are advanced or delayed under LL or DD; they have similar circadian locomotor rhythms to controls under light-dark (LD) and when shifted to DD conditions with no circadian temperature cycle; sensory activity in the hind leg nerve in response to temperature changes is lost, compared to controls, with no alteration in motor activity or spontaneous leg movement.

      Ir25a2/Ir25a1 adults have a reduced phase-shift in circadian locomotor activity, compared to controls, when light-dark entrained flies are moved to dark-dark with 7 h advanced 25[o] C:27 [o]C temperature cycles.

      Ir25a2/Df(2L)Exel6010 adults have a significantly lower circadian clock entrainment index for locomotor activity rhythms following a 7 h delay under 25[o] C:27 [o]C temperature cycles in constant light conditions, compared to controls.

      (Chen et al., 2015)

      Mutant flies show normal responses to both low salt (50mM NaCl) and high salt (500mM NaCl) in a two-way choice test.

      (Zhang et al., 2013)

      Homozygous Ir25a2 mutant animals are viable and fertile.

      Ir25a2 mutant class 4 (ac4) coeloconic sensilla lack electrophysiological responsiveness to phenylethyl amine, while phenylacetaldehyde responses are unchanged.

      Ir25a2 mutant class 3 (ac3) coeloconic sensilla exhibit normal electrophysiological responsiveness to propionic acid.

      Electrophysiological responses to γ-hexalactone persist in Ir25a2 mutant class 3 (ac3) coeloconic sensilla.

      Electrophysiological responses to 1,4-diaminobutane, but not to acetic acid, are abolished in Ir25a2 mutant class 2 (ac2) coeloconic sensilla.

      Electrophysiological responses to ammonia are retained in Ir25a2 mutant class 1 (ac1) coeloconic sensilla.

      (Abuin et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Ir25a2 has abnormal behavior | larval stage phenotype, non-enhanceable by Fa2hKO1

      (Li et al., 2024)
      NOT suppressed by
      Statement
      Reference

      Ir25a2 has abnormal behavior | larval stage phenotype, non-suppressible by Fa2hKO1

      (Li et al., 2024)
      Suppressor of
      Statement
      Reference

      Ir25a2 is a suppressor | partially of abnormal behavior | larval stage phenotype of Fa2hKO1

      (Li et al., 2024)

      Ir25a2 is a suppressor of abnormal neurophysiology | adult stage phenotype of Ir56bUAS.cDa, Scer\GAL4Gr33a.PU

      (Dweck et al., 2022)
      NOT Suppressor of
      Statement
      Reference

      Ir25a2 is a non-suppressor of viable phenotype of Ir8a1

      (Abuin et al., 2011)

      Ir25a2 is a non-suppressor of fertile phenotype of Ir8a1

      (Abuin et al., 2011)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Ir8a1, Ir25a2 double mutant animals are viable and fertile.

      (Abuin et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Generated by gene targeting at the Ir25a locus.

      (Benton et al., 2009)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      References (48)