Open Close
General Information
Symbol
Dmel\Ir25a2
Species
D. melanogaster
Name
FlyBase ID
FBal0220383
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
TI{TI}Ir25a2
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ir25a2 mutant flies do not show significant changes in the expected avoidance response to food laced with different acids (5% acetic acid, 5% glycolic acid, 10% citric acid, 20% lactic acid, 5% tartaric acid, 2% propionic acid, 2% butyric acid, or 10mM HCl), as compared to controls. GRNs enervating S6 and S10 sensilla do not show changes in the responses (frequency of spikes) to acetic acid (1%), glycolic acid (1%), tartaric acid (1%), citric acid (5%), propionic acid (1%), butyric acid (1%), HCl (10mM), and lactic acid (20%), as compared to controls.

Ir25a2/Ir25a2 adult females have significantly reduced preference for oviposition on acid-containing medium, compared to controls.

Ir25a2/Ir25a2 mutant females do not exhibit a significant reduction in feeding preference index for 1% yeast extract versus 5mM sucrose solution, as compared to wild type.

Ir25a2 homozygous mutant larvae do not have any alteration in amino acid preference, compared to controls.

In olfactory preference assays for polyamines, Ir25a2/Ir25a2 flies do not show any significant difference in their attraction to putrescine or cadaverine, as compared to wild type, and females do not show any significant difference in their aversion to oviposit on polyamine-rich substrate (plain, sugar-free 1% agarose supplemented with either putrescine or cadaverine) versus non-supplemented substrate, as compared to wild type.

Ir25a2 mutant larvae have significantly decreased bitter avoidance, compared to controls.

Ir25a2 homozygous adults are able to synchronize circadian locomotor activity to high amplitude (4[o]C) temperature cycles under constant light (LL) or constant dark (DD) conditions, similar to controls; at a range of smaller (2[o]C) temperature intervals, Ir25a2 adults fail to synchronize to temperature cycles under LL; they also have a significantly reduced phase-shift in circadian locomotor activity, compared to controls, when shallow (2[o]C) temperature cycles are advanced or delayed under LL or DD; they have similar circadian locomotor rhythms to controls under light-dark (LD) and when shifted to DD conditions with no circadian temperature cycle; sensory activity in the hind leg nerve in response to temperature changes is lost, compared to controls, with no alteration in motor activity or spontaneous leg movement.

Ir25a2/Ir25a1 adults have a reduced phase-shift in circadian locomotor activity, compared to controls, when light-dark entrained flies are moved to dark-dark with 7 h advanced 25[o] C:27 [o]C temperature cycles.

Ir25a2/Df(2L)Exel6010 adults have a significantly lower circadian clock entrainment index for locomotor activity rhythms following a 7 h delay under 25[o] C:27 [o]C temperature cycles in constant light conditions, compared to controls.

Mutant flies show normal responses to both low salt (50mM NaCl) and high salt (500mM NaCl) in a two-way choice test.

Homozygous Ir25a2 mutant animals are viable and fertile.

Ir25a2 mutant class 4 (ac4) coeloconic sensilla lack electrophysiological responsiveness to phenylethyl amine, while phenylacetaldehyde responses are unchanged.

Ir25a2 mutant class 3 (ac3) coeloconic sensilla exhibit normal electrophysiological responsiveness to propionic acid.

Electrophysiological responses to γ-hexalactone persist in Ir25a2 mutant class 3 (ac3) coeloconic sensilla.

Electrophysiological responses to 1,4-diaminobutane, but not to acetic acid, are abolished in Ir25a2 mutant class 2 (ac2) coeloconic sensilla.

Electrophysiological responses to ammonia are retained in Ir25a2 mutant class 1 (ac1) coeloconic sensilla.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Statement
Reference

Ir25a2 is a non-suppressor of viable phenotype of Ir8a1

Ir25a2 is a non-suppressor of fertile phenotype of Ir8a1

Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Ir8a1, Ir25a2 double mutant animals are viable and fertile.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of Ir25aScer\UAS.cAa driven by Scer\GAL4Ir25a.PA partially rescues the odor-evoked electrophysiological response phenotypes in Ir25a2 mutant coeloconic sensilla.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Generated by gene targeting at the Ir25a locus.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (24)