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General Information
Symbol
Dmel\LrrkI1915T.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0220788
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dLRRK I1915T, UAS-dLRRK-I1915T
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

The I1915T mutation was reported relative to Lrrk-RB. analogous mutation in human LRRK2 implicated in Parkinson;disease 8; mutation carried on in vitro construct; site of nucleotide substitution in fly gene and specific disease association inferred by FlyBase curator.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: I1915T.

UAS regulatory sequences drive expression of a mutated form of Lrrk.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
This allele represents a human variant implicated in disease.
LRRK2:p.Ile2020Thr
Variants Synonym(s)
Associated human disease model(s)
External database links
Comments concerning this variant
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4ple.PG do not show a progressive reduction in electroretinogram amplitude (there is no significant difference in the amplitude seen in 3 and 28 day old flies).

Expression of LrrkI1915T.Scer\UAS driven by Scer\GAL4ple.PF leads to dopaminergic neuron loss.

Expression of LrrkI1915T.Scer\UAS under the control of Scer\GAL4GMR.PF does not result in any apparent eye degeneration.

Pre-synaptic or post-synaptic expression of LrrkI1915T.Scer\UAS (under the control of either Scer\GAL4elav-C155 or Scer\GAL4Mhc.PW) results in an estimated 20% decrease in the total number of type I boutons and neuromuscular junction branch number on muscle 6/7 of A3, with no observable effect on muscle size. Neuromuscular length is normal.

60 day old adults expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4ple.PF show a significant reduction in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster compared to control flies, although the number of neurons in the protocerebral posterior medial 3 cluster is unaffected in the mutant flies.

Flies expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4da.G32 show significantly higher sensitivity to paraquat and to hydrogen peroxide compared to control flies.

Expression of LrrkI1915T.Scer\UAS under the control of Scer\GAL4elav-C155 results in locomotor dysfunction with age (45 day old flies show significantly reduced climbing ability compared to controls).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

LrrkI1915T.UAS, Scer\GAL4elav.Switch.PO, foxoUAS.cKb has dopaminergic neuron | RU486 conditional phenotype, suppressible by W[+]/hid1

LrrkI1915T.UAS, Scer\GAL4elav.Switch.PO, foxoUAS.cKb has dopaminergic PPL1 neuron | RU486 conditional phenotype, suppressible by W[+]/hid1

NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The dot-like small eye phenotype characteristic for flies expressing egrScer\UAS.cMa under the control of Scer\GAL4GMR.PFa is strongly suppressed by co-expression of LrrkI1915T.Scer\UAS.

Co-expression of foxoScer\UAS.cPa and forP1.Scer\UAS under the control of Scer\GAL4GMR.PF results in degeneration of the eye. The severity of this phenotype is enhanced by co-expression of LrrkI1915T.Scer\UAS.

Co-expression of forP1.Scer\UAS and LrrkI1915T.Scer\UAS under the control of Scer\GAL4GMR.PF results in flies with normal eyes.

Co-expression of foxoScer\UAS.cPa and forP1.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 results in loss of dopaminergic neurons. This phenotype is enhanced by co-expression of LrrkI1915T.Scer\UAS.

Co-expression of foxoScer\UAS.cPa and forP1.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 results in flies with reduced climbing ability. This phenotype is worsened by co-expression of LrrkI1915T.Scer\UAS.

In flies expressing LrrkI1915T.Scer\UAS driven by Scer\GAL4ple.PF, Dcr-1Scer\UAS.cDa-overexpression suppresses the neuronal toxicity of LrrkI1915T.Scer\UAS.

In flies expressing LrrkI1915T.Scer\UAS driven by Scer\GAL4ple.PF, Dcr-1VDRC.cUa-overexpression exacerbates the neuronal toxicity of LrrkI1915T.Scer\UAS.

Heterozygosity for E2f07172 suppresses the dopaminergic neuronal phenotypes in animals expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4ple.PF.

Heterozygosity for Dp49Fk-1 suppresses the dopaminergic neuronal phenotypes in animals expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4ple.PF.

Overexpression of RbfScer\UAS.cDa suppresses the dopaminergic neuronal phenotypes in animals expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4ple.PF.

Heterozygosity for lkb14A4-2 has no effect on the dopaminergic neuronal phenotypes in animals expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4ple.PF.

Overexpression of let-7Scer\UAS.cGa partly suppresses the dopaminergic neuronal phenotypes of LrrkI1915T.Scer\UAS-expression via Scer\GAL4ple.PF.

Overexpression of mir-184Scer\UAS.cGa partly suppresses the dopaminergic neuronal phenotypes of LrrkI1915T.Scer\UAS-expression via Scer\GAL4ple.PF.

The severity of the eye defects caused by expression of foxoScer\UAS.cKb under the control of Scer\GAL4GMR.PF are enhanced by co-expression of LrrkI1915T.Scer\UAS.

Co-expression of foxoScer\UAS.cKb and LrrkI1915T.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 throughout the lifespan results in a reduction in lifespan. These flies show an age-dependent decrease in the number of PPM1/2, PPM3 and PPL1 neurons and 1 day old flies show a defect in climbing ability.

Co-expression of foxoS259A.Scer\UAS and LrrkI1915T.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 throughout the lifespan does not have an effect on lifespan. The flies do not show loss of dopaminergic neurons PAL, PPM1/2, PPM3 or PPL1.

The eye phenotype caused by co-expression of foxoScer\UAS.cKb and LrrkI1915T.Scer\UAS under the control of Scer\GAL4GMR.PF is significantly suppressed if the flies also carry either NcKG02994/+ or W1/+.

The eye phenotype caused by co-expression of foxoScer\UAS.cKb and LrrkI1915T.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced by th1/+.

The degeneration of the PPM1/2 and PPL1 neurons which is seen in flies co-expressing foxoScer\UAS.cKb and LrrkI1915T.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 is suppressed if they alo carry W1/+.

The increased sensitivity to paraquat that is seen in flies expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4da.G32 is suppressed to wild-type levels of sensitivity if the flies are also heterozygous for eIF-4E07238.

The decrease in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster that is seen in 60 day old adults expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4ple.PF is suppressed by co-expression of ThorScer\UAS.cMa.

The reduced climbing ability that is seen in 45 day old flies expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4elav-C155 is partially rescued by co-expression of ThorTA.Scer\UAS.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
LrrkI1915T.Scer\UAS
LrrkI1915T.UAS
dLRRK I1915T
dLRRKI1915T
Name Synonyms
Secondary FlyBase IDs
    References (11)