Cbp20e02787/Smnf01109 or FMRFaKG01300/+;Smnf01109/+ third instar larvae show significant decreases in the number of boutons per muscle area at the neuromuscular junction, compared to heterozygotes (heterozygotes are similar to wild type).
Expression of witScer\UAS.cMa under the control of Scer\GAL4elav.PLu in a Smnf01109/+ background results in a reduction in bouton number per muscle area at the third larval instar neuromuscular junction compared to wild type.
Expression of SmnT:Zzzz\FLAG in a Smnf01109/SmnX7 mutant background results in >70% rescue of lethality. The resulting adults are fertile with no apparent defects in flight or motility. Pupation and eclosion occurs a day later than in wild type controls. Expression of SmnT:Zzzz\FLAG also rescues the motility and burrowing defects seen in Smnf01109/SmnX7 mutant larvae.
Expression of SmnT205I.T:Zzzz\FLAG fully rescues the larval lethality seen in Smnf01109/SmnX7 mutants. However lethality in these mutants occurs at the pupal stage, with only 25% of mutants eclosing as adults. Pupation and eclosion occurs a day later than in wild type controls. Expression of SmnT:Zzzz\FLAG also largely rescues the motility defects seen in Smnf01109/SmnX7 mutant larvae, whereas the burrowing defects are fully rescued.