Open Close
General Information
Symbol
Dmel\Smnf01109
Species
D. melanogaster
Name
FlyBase ID
FBal0220799
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
SmnD, f01109
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Insertion within the coding region, at amino acid residue I93.

    Insertion at +58bp from the translation start.

    Insertion components
    PBac{WH}Smnf01109
    Product class / Tool use(s)
    Encoded product / tool
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    20-30% of Smnf01109/SmnX7 mutant larvae are long lived and can survive for many days without undergoing metamorphosis or exhibiting the wandering behaviour typical of the late third instar.

    Smnf01109/SmnX7 mutant animals display significant defects in larval locomotion. Larvae that have been placed on their dorsal surface take longer to return to their ventral surface (the crawling position ) than controls. Smnf01109/SmnX7 mutant larvae are also unable to burrow into their food although if cuts are made in the media, the larvae are able to work their way into the crevices.

    Smn73Ao/Smnf01109 third instar larvae show significant decreases in bouton number (per muscle area) compared to either heterozygote (heterozygotes are similar to controls).

    Homozygous larvae are thinner and shorter than control larvae at 4 days after egg laying.

    Homozygous second-third instar larvae show impaired motor function, showing a smaller distance travelled in the 20 seconds after movement was stimulated with a needle than control larvae.

    67% of homozygotes eclose as adults.

    Homozygous third instar larvae show a moderate reduction in bouton number per muscle area at the neuromuscular junction compared to wild type.

    Smn73Ao/Smnf01109 third instar larvae show a reduction in bouton number per muscle area at the neuromuscular junction compared to wild type.

    Smnf05960/Smnf01109 third instar larvae do not show a change in bouton number per muscle area at the neuromuscular junction compared to wild type.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Cbp20e02787/Smnf01109 or FMRFaKG01300/+;Smnf01109/+ third instar larvae show significant decreases in the number of boutons per muscle area at the neuromuscular junction, compared to heterozygotes (heterozygotes are similar to wild type).

    Expression of witScer\UAS.cMa under the control of Scer\GAL4elav.PLu in a Smnf01109/+ background results in a reduction in bouton number per muscle area at the third larval instar neuromuscular junction compared to wild type.

    Smnf01109/Mad12 double heterozygotes show a reduction in bouton number per muscle area at the third larval instar neuromuscular junction compared to wild type.

    The reduction in bouton number per muscle area at the neuromuscular junction that is seen in Smn73Ao/Smnf01109 third instar larvae is suppressed by Dad271-68/Dad271-68.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Not rescued by
    Comments

    Expression of SmnT:Zzzz\FLAG in a Smnf01109/SmnX7 mutant background results in >70% rescue of lethality. The resulting adults are fertile with no apparent defects in flight or motility. Pupation and eclosion occurs a day later than in wild type controls. Expression of SmnT:Zzzz\FLAG also rescues the motility and burrowing defects seen in Smnf01109/SmnX7 mutant larvae.

    Expression of SmnAct5C.T:Zzzz\FLAG fails to rescue the larval lethality seen in Smnf01109/SmnX7 mutants.

    Expression of SmnT205I.T:Zzzz\FLAG fully rescues the larval lethality seen in Smnf01109/SmnX7 mutants. However lethality in these mutants occurs at the pupal stage, with only 25% of mutants eclosing as adults. Pupation and eclosion occurs a day later than in wild type controls. Expression of SmnT:Zzzz\FLAG also largely rescues the motility defects seen in Smnf01109/SmnX7 mutant larvae, whereas the burrowing defects are fully rescued.

    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    References (7)