FlyBase Allele Report: Dmel\Smn[f01109]

20-30% of Smn^f01109/Smn^X7 mutant larvae are long lived and can survive for many days without undergoing metamorphosis or exhibiting the wandering behaviour typical of the late third instar.

Smn^f01109/Smn^X7 mutant animals display significant defects in larval locomotion. Larvae that have been placed on their dorsal surface take longer to return to their ventral surface (the crawling position ) than controls. Smn^f01109/Smn^X7 mutant larvae are also unable to burrow into their food although if cuts are made in the media, the larvae are able to work their way into the crevices.

(Praveen et al., 2012)

Smn^73Ao/Smn^f01109 third instar larvae show significant decreases in bouton number (per muscle area) compared to either heterozygote (heterozygotes are similar to controls).

(Dimitriadi et al., 2010)

Homozygous larvae are thinner and shorter than control larvae at 4 days after egg laying.

Homozygous second-third instar larvae show impaired motor function, showing a smaller distance travelled in the 20 seconds after movement was stimulated with a needle than control larvae.

(Shpargel et al., 2009)

67% of homozygotes eclose as adults.

Homozygous third instar larvae show a moderate reduction in bouton number per muscle area at the neuromuscular junction compared to wild type.

Smn^73Ao/Smn^f01109 third instar larvae show a reduction in bouton number per muscle area at the neuromuscular junction compared to wild type.

Smn^f05960/Smn^f01109 third instar larvae do not show a change in bouton number per muscle area at the neuromuscular junction compared to wild type.

(Chang et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Cbp20[+]/Cbp20^e02787, Smn^f01109 has abnormal neuroanatomy | third instar larval stage phenotype

(Dimitriadi et al., 2010)

FMRFa[+]/FMRFa^KG01300, Smn^f01109 has abnormal neuroanatomy | third instar larval stage phenotype

(Dimitriadi et al., 2010)

Scer\GAL4^elav.PLu, Smn^f01109, wit^UAS.cMa has abnormal neuroanatomy | third instar larval stage phenotype

(Chang et al., 2008)

Mad¹²/Mad[+], Smn^f01109 has abnormal neuroanatomy | third instar larval stage phenotype

(Chang et al., 2008)

Phenotype Manifest In

Other

Statement

Reference

Cbp20[+]/Cbp20^e02787, Smn^f01109 has NMJ bouton | third instar larval stage phenotype

(Dimitriadi et al., 2010)

FMRFa[+]/FMRFa^KG01300, Smn^f01109 has NMJ bouton | third instar larval stage phenotype

(Dimitriadi et al., 2010)

Scer\GAL4^elav.PLu, Smn^f01109, wit^UAS.cMa has NMJ bouton phenotype

(Chang et al., 2008)

Mad¹²/Mad[+], Smn^f01109 has NMJ bouton phenotype

(Chang et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Cbp20^e02787/Smn^f01109 or FMRFa^KG01300/+;Smn^f01109/+ third instar larvae show significant decreases in the number of boutons per muscle area at the neuromuscular junction, compared to heterozygotes (heterozygotes are similar to wild type).

(Dimitriadi et al., 2010)

Expression of wit^Scer\UAS.cMa under the control of Scer\GAL4^elav.PLu in a Smn^f01109/+ background results in a reduction in bouton number per muscle area at the third larval instar neuromuscular junction compared to wild type.

Smn^f01109/Mad¹² double heterozygotes show a reduction in bouton number per muscle area at the third larval instar neuromuscular junction compared to wild type.

The reduction in bouton number per muscle area at the neuromuscular junction that is seen in Smn^73Ao/Smn^f01109 third instar larvae is suppressed by Dad^271-68/Dad^271-68.

(Chang et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Smn^X7/Smn^f01109 is partially rescued by Smn^Tag:FLAG

(Praveen et al., 2012)

Smn^X7/Smn^f01109 is partially rescued by Smn^{T205I.Tag:FLAG}

(Praveen et al., 2012)

Smn^73Ao/Smn^f01109 is partially rescued by Smn^UAS.Tag:FLAG/Scer\GAL4^elav.PLu

(Chang et al., 2008)

Smn^73Ao/Smn^f01109 is partially rescued by Scer\GAL4^how-24B/Smn^UAS.Tag:FLAG/Scer\GAL4^how-24B

(Chang et al., 2008)

Not rescued by

Smn^X7/Smn^f01109 is not rescued by Smn^{Act5C.Tag:FLAG}

(Praveen et al., 2012)

Comments

Expression of Smn^T:Zzzz\FLAG in a Smn^f01109/Smn^X7 mutant background results in >70% rescue of lethality. The resulting adults are fertile with no apparent defects in flight or motility. Pupation and eclosion occurs a day later than in wild type controls. Expression of Smn^T:Zzzz\FLAG also rescues the motility and burrowing defects seen in Smn^f01109/Smn^X7 mutant larvae.

Expression of Smn^{Act5C.T:Zzzz\FLAG} fails to rescue the larval lethality seen in Smn^f01109/Smn^X7 mutants.

Expression of Smn^{T205I.T:Zzzz\FLAG} fully rescues the larval lethality seen in Smn^f01109/Smn^X7 mutants. However lethality in these mutants occurs at the pupal stage, with only 25% of mutants eclosing as adults. Pupation and eclosion occurs a day later than in wild type controls. Expression of Smn^T:Zzzz\FLAG also largely rescues the motility defects seen in Smn^f01109/Smn^X7 mutant larvae, whereas the burrowing defects are fully rescued.

(Praveen et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

85617

w¹¹¹⁸; PBac{WH}Smn^f01109

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Smn^D

(Spring et al., 2019, Garcia et al., 2013, Praveen et al., 2012, Shpargel et al., 2009, Rajendra et al., 2007)

Smn^f01109

(Dimitriadi et al., 2010, Chang et al., 2008)

f01109

(Garcia et al., 2013, Praveen et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (7)

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