The survival rate and growth of Max1 larvae is not reduced if the animals are also expressing MaxdsRNA.Scer\UAS.IR under the control of Scer\GAL4Act5C.PI.
Expression of one copy of MaxdsRNA.Scer\UAS.IR under the control of Scer\GAL4Act5C.PI results in complete lethality at the pupal stage in the presence of Max1.
Eyes largely consisting of homozygous clones develop and grow as well as control eyes.
Bristles lacking Max1 function (induced as a somatic clone) are severely stunted in their growth compared to controls.
Homozygous clones in the fat body result in fat body cells that are only moderately reduced in overall size and that have nuclei that are only moderately reduced in size. These cells are often indistinguishable from surrounding wild-type cells.
Homozygotes survive embryogenesis and hatch at the same proportions as controls. Most homozygotes survive until the pupal stages, although homozygous larvae show some reduction in viability compared to controls. 42% of homozygous pupae undergo metamorphosis, becoming morphologically normal pharate adults, but these animals never eclose.
Homozygotes are smaller than controls at the larval, pupal and pharate adult stages.
Homozygotes show a normal rate of development up to the molt from second to third instar, but homozygous third instar larvae pupariate only between days 8 and 10 after egg deposition (wild-type animals pupariate at day 5 after egg deposition).
Homozygous third instar larvae have fewer teeth on their mouth hooks than normal, and occasionally the tips of these mouth hooks are forked. Most pupae fail to evert their anterior spiracles.
Max1 larval fat body and salivary gland cells grow markedly between days 5 and 8 after egg deposition and reach near wild-type sizes.
Homozygous 72 hour old clones in the imaginal discs of wandering third instar larvae are not significantly different in size from their wild-type twin spots and are made up of normally sized cells.
Homozygous clones in the eye generated using the ey-FLP system can occupy a large fraction of the eye, as occurs with wild-type clones generated using this method.