FlyBase Allele Report: Dmel\Max[1]

General Information

Symbol

Dmel\Max¹

Species

D. melanogaster

Name

FlyBase ID

FBal0230159

Feature type

allele

Associated gene

Dmel\Max

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Steiger et al., 2008)

Mutagen

P-element activity

(Steiger et al., 2008)

Progenitor genotype

P{EPgy2}Mettl5^EY02775

(Steiger et al., 2008)

Caused by aberration

Df(3L)Max¹

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 1804bp deletion extending from nucleotide 19255898 to nucleotide 19257703 (coordinates according to GenBank accession AE014296). The deletion removes the entire Max open reading frame. The deletion also disrupts the neighbouring CG9666 gene.

(Steiger et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:19,262,798..19,264,603 [-]

Comment:

An 1804 bp deletion resulting from the imprecise excision of P{EPgy2}Mettl5^EY02775 extending from nt 19255898 to nt 19257703 of AE014296.4. Deletion affects both Max and Mettl5.

(Steiger et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior pupal spiracle

(Steiger et al., 2008)

chaeta | somatic clone

(Steiger et al., 2008)

mouth hook | third instar larval stage

(Steiger et al., 2008)

Detailed Description

Statement

Reference

The survival rate and growth of Max¹ larvae is not reduced if the animals are also expressing Max^{dsRNA.Scer\UAS.IR} under the control of Scer\GAL4^Act5C.PI.

Expression of one copy of Max^{dsRNA.Scer\UAS.IR} under the control of Scer\GAL4^Act5C.PI results in complete lethality at the pupal stage in the presence of Max¹.

Eyes largely consisting of homozygous clones develop and grow as well as control eyes.

Bristles lacking Max¹ function (induced as a somatic clone) are severely stunted in their growth compared to controls.

Homozygous clones in the fat body result in fat body cells that are only moderately reduced in overall size and that have nuclei that are only moderately reduced in size. These cells are often indistinguishable from surrounding wild-type cells.

(Steiger et al., 2008)

Homozygotes survive embryogenesis and hatch at the same proportions as controls. Most homozygotes survive until the pupal stages, although homozygous larvae show some reduction in viability compared to controls. 42% of homozygous pupae undergo metamorphosis, becoming morphologically normal pharate adults, but these animals never eclose.

Homozygotes are smaller than controls at the larval, pupal and pharate adult stages.

Homozygotes show a normal rate of development up to the molt from second to third instar, but homozygous third instar larvae pupariate only between days 8 and 10 after egg deposition (wild-type animals pupariate at day 5 after egg deposition).

Homozygous third instar larvae have fewer teeth on their mouth hooks than normal, and occasionally the tips of these mouth hooks are forked. Most pupae fail to evert their anterior spiracles.

Max¹ larval fat body and salivary gland cells grow markedly between days 5 and 8 after egg deposition and reach near wild-type sizes.

Homozygous 72 hour old clones in the imaginal discs of wandering third instar larvae are not significantly different in size from their wild-type twin spots and are made up of normally sized cells.

Homozygous clones in the eye generated using the ey-FLP system can occupy a large fraction of the eye, as occurs with wild-type clones generated using this method.

(Steiger et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Max¹ is a suppressor of lethal | recessive phenotype of Myc⁴

(Steiger et al., 2008)

Max¹ is a suppressor of decreased body size | larval stage phenotype of Myc⁴

(Steiger et al., 2008)

Other

Statement

Reference

Max¹, Myc^P0 has abnormal developmental rate phenotype

(Steiger et al., 2008)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

dm^P0/Y ; Max¹/+ flies take slightly longer for their development than controls.

(Steiger et al., 2008)

dm⁴ Max¹ and dm⁴ Mnt¹ Max¹ larvae grow better and survive to a greater extent than dm⁴ single mutant larvae.

Clones in the wing disc expressing wild-type levels of dm in a Max¹ background and in which the surrounding wing disc cells are expressing dm^αTub84B.PBb are smaller than genetically identical clones in the wing disc that are in a Max¹ background and in which the surrounding wing disc cells express normal levels of dm.

(Steiger et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Max¹ is rescued by Max^+t2.788

(Steiger et al., 2008)

Comments

Images (0)

Mutant

Wild-type