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General Information
Symbol
Dmel\DopEcRKK111211
Species
D. melanogaster
Name
FlyBase ID
FBal0237281
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-DopEcR RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of DopEcRKK111211 under the control of Scer\GAL4da.PU leads to significant resistance to ethanol-induced sedation (the flies take longer to cease all movements upon sedation compared to controls). Using tub-Gal80[ts] to limit the time of RNAi expression to the developmental stage - i.e. before eclosion - does not affect sedation sensitivity in the adults, whereas RNAi knockdown after eclosion does increase resistance to ethanol-induced sedation. The increased resistance to sedation is also observed in flies expressing DopEcRKK111211 under the control of any of these drivers: Scer\GAL4elav.PU, Scer\GAL4ChAT.PU or Scer\GAL4dimm-929 but no significant effect is seen when the RNAi is driven by any of the following: Scer\GAL4c747, Scer\GAL4ple.PU or Scer\GAL4Ilp2.PU.

Expression of DopEcRKK111211 driven by Scer\GAL4GMR23E10 does not significantly affect sleep amount per day, compared to controls.

Males expressing DopEcRKK111211 under the control of Scer\GAL4αTub84B.Switch.PK in the presence of RU486 lack 30-minute courtship memory in a courtship conditioning assay. This defect is not suppressed if the males are fed 0.1mM 20-hydroxy-ecdysone immediately prior to conditioning.

Males expressing DopEcRKK111211 under the control of either Scer\GAL4c747 or Scer\GAL4c739 lack 30-minute courtship memory in a courtship conditioning assay.

Unlike in wild type, female flies expressing DopEcRKK111211 under the control of the pan-neuronal driver Scer\GAL4n-syb.PS do not show an increase in sugar-sensitive proboscis extension reflex (PER) in response to L-Dopa feeding. The same effect is seen when DopEcRKK111211 is expressed in Gr5a gustatory receptor neurons (GRNs) under the control of Scer\GAL4Gr5a.8.5. A greater proportion of flies expressing DopEcRKK111211 under the control of Scer\GAL4Gr5a.8.5 also exhibit a PER following 6 hours of wet starvation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The increased sensitivity of mldDTS3-1/+ heterozygote adults (reared at 18[o]C during development and transferred to 29[o]C after eclosion) to ethanol-induced sedation is suppressed by expression of DopEcRKK111211 under the control of Scer\GAL4da.PU in the mutant background. The double mutants display increased resistance to ethanol sedation which is close to that seen in DopEcRKK111211 RNAi flies alone.

Males expressing DopEcRKK111211 under the control of Scer\GAL4αTub84B.Switch.PK in a dnc1 background in the presence of RU486 show 30-minute courtship memory in a courtship conditioning assay.

Xenogenetic Interactions
Statement
Reference

The accelerated age-progressive decline of locomotor ability of adult flies expressing either Hsap\HTTNT.128Q.Scer\UAS or Hsap\HTT200Q.Scer\UAS under the control of Scer\GAL4elav-C155 is strongly improved by co-expression of DopEcRKK111211.

Complementation and Rescue Data
Partially rescued by
Comments

The increased resistance to ethanol-induced sedation characteristic for adult flies expressing DopEcRKK111211 under the control of Scer\GAL4da.PU is partially suppressed by co-expression of DopEcRScer\UAS.cIb.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
DopEcRKK103494
DopEcRKK111211
Name Synonyms
Secondary FlyBase IDs
    References (10)