FlyBase Allele Report: Dmel\DopEcR[KK111211]

General Information

Symbol

Dmel\DopEcR^KK111211

Species

D. melanogaster

Name

FlyBase ID

FBal0237281

Feature type

allele

Associated gene

Dmel\DopEcR

Associated Insertion(s)

Carried in Construct

P{KK111211}

Also Known As

UAS-DopEcR RNAi

Key Links

Genomic Maps

GBrowse JBrowse

Help me choose

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Keleman et al., 2009.8.5)

in vitro construct - regulatory fusion

(Keleman et al., 2009.8.5)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{KK111211}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

DopEcR

Associated Sequence Features

dsRNA-KK111211

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^NT.128Q.UAS

(Yao et al., 2015)

modeled by Hsap\HTT^200Q.UAS

(Yao et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage, with Scer\GAL4^c739

(Ishimoto et al., 2013)

abnormal memory | adult stage, with Scer\GAL4^c747

(Ishimoto et al., 2013)

abnormal memory | adult stage | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

(Ishimoto et al., 2013)

abnormal tarsal response | chemical conditional, with Scer\GAL4^Gr5a.8.5

(Inagaki et al., 2012)

abnormal tarsal response | chemical conditional, with Scer\GAL4^nSyb.PS

(Inagaki et al., 2012)

abnormal tarsal response | nutrition conditional, with Scer\GAL4^Gr5a.8.5

(Inagaki et al., 2012)

chemical resistant | adult stage, with Scer\GAL4^ChAT.PU

(Petruccelli et al., 2016)

chemical resistant | adult stage, with Scer\GAL4^da.PU

(Petruccelli et al., 2016)

chemical resistant | adult stage, with Scer\GAL4^dimm-929

(Petruccelli et al., 2016)

chemical resistant | adult stage, with Scer\GAL4^elav.PU

(Petruccelli et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of DopEcR^KK111211 under the control of Scer\GAL4^da.PU leads to significant resistance to ethanol-induced sedation (the flies take longer to cease all movements upon sedation compared to controls). Using tub-Gal80[ts] to limit the time of RNAi expression to the developmental stage - i.e. before eclosion - does not affect sedation sensitivity in the adults, whereas RNAi knockdown after eclosion does increase resistance to ethanol-induced sedation. The increased resistance to sedation is also observed in flies expressing DopEcR^KK111211 under the control of any of these drivers: Scer\GAL4^elav.PU, Scer\GAL4^ChAT.PU or Scer\GAL4^dimm-929 but no significant effect is seen when the RNAi is driven by any of the following: Scer\GAL4^c747, Scer\GAL4^ple.PU or Scer\GAL4^Ilp2.PU.

(Petruccelli et al., 2016)

Expression of DopEcR^KK111211 driven by Scer\GAL4^GMR23E10 does not significantly affect sleep amount per day, compared to controls.

(Pimentel et al., 2016)

Males expressing DopEcR^KK111211 under the control of Scer\GAL4^{αTub84B.Switch.PK} in the presence of RU486 lack 30-minute courtship memory in a courtship conditioning assay. This defect is not suppressed if the males are fed 0.1mM 20-hydroxy-ecdysone immediately prior to conditioning.

Males expressing DopEcR^KK111211 under the control of either Scer\GAL4^c747 or Scer\GAL4^c739 lack 30-minute courtship memory in a courtship conditioning assay.

(Ishimoto et al., 2013)

Unlike in wild type, female flies expressing DopEcR^KK111211 under the control of the pan-neuronal driver Scer\GAL4^n-syb.PS do not show an increase in sugar-sensitive proboscis extension reflex (PER) in response to L-Dopa feeding. The same effect is seen when DopEcR^KK111211 is expressed in Gr5a gustatory receptor neurons (GRNs) under the control of Scer\GAL4^Gr5a.8.5. A greater proportion of flies expressing DopEcR^KK111211 under the control of Scer\GAL4^Gr5a.8.5 also exhibit a PER following 6 hours of wet starvation.

(Inagaki et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

DopEcR^KK111211, Scer\GAL4^{αTub84B.Switch.PK} has abnormal memory | adult stage | RU486 conditional phenotype, suppressible by dnc¹

(Ishimoto et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/DopEcR^KK111211 is a suppressor of chemical sensitive | adult stage | temperature conditional phenotype of mld^DTS3-1

(Petruccelli et al., 2016)

DopEcR^KK111211, Scer\GAL4^elav-C155 is a suppressor | partially of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\HTT^NT.128Q.UAS, Scer\GAL4^elav-C155

(Yao et al., 2015)

DopEcR^KK111211, Scer\GAL4^elav-C155 is a suppressor | partially of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\HTT^200Q.UAS, Scer\GAL4^elav-C155

(Yao et al., 2015)

DopEcR^KK111211/Scer\GAL4^{αTub84B.Switch.PK} is a suppressor | RU486 conditional of abnormal memory | adult stage phenotype of dnc²

(Ishimoto et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The increased sensitivity of mld^DTS3-1/+ heterozygote adults (reared at 18[o]C during development and transferred to 29[o]C after eclosion) to ethanol-induced sedation is suppressed by expression of DopEcR^KK111211 under the control of Scer\GAL4^da.PU in the mutant background. The double mutants display increased resistance to ethanol sedation which is close to that seen in DopEcR^KK111211 RNAi flies alone.

(Petruccelli et al., 2016)

Males expressing DopEcR^KK111211 under the control of Scer\GAL4^{αTub84B.Switch.PK} in a dnc¹ background in the presence of RU486 show 30-minute courtship memory in a courtship conditioning assay.

(Ishimoto et al., 2013)

Xenogenetic Interactions

Statement

Reference

The accelerated age-progressive decline of locomotor ability of adult flies expressing either Hsap\HTT^{NT.128Q.Scer\UAS} or Hsap\HTT^{200Q.Scer\UAS} under the control of Scer\GAL4^elav-C155 is strongly improved by co-expression of DopEcR^KK111211.

(Yao et al., 2015)

Complementation and Rescue Data

Partially rescued by

DopEcR^KK111211 is partially rescued by Scer\GAL4^da.PU/DopEcR^UAS.cIb

(Petruccelli et al., 2016)

Comments

The increased resistance to ethanol-induced sedation characteristic for adult flies expressing DopEcR^KK111211 under the control of Scer\GAL4^da.PU is partially suppressed by co-expression of DopEcR^Scer\UAS.cIb.

(Petruccelli et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v103494

P{KK111211}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

DopEcR^KK103494

(Yao et al., 2015)

DopEcR^KK111211

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

Open Close