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General Information
Symbol
Dmel\hbsdsRNA.UAS.cBa
Species
D. melanogaster
Name
FlyBase ID
FBal0240396
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences control expression of an inverted repeated hbs (167-670 bp) for RNAi.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Moderate reduction of hbs through the expression of hbsIR.Scer\UAS under the control of Scer\GAL4GMR.PF results in defects in inter-ommatidial cells and bristle group positioning.

Strong reduction of hbsIR.Scer\UAS under the control of Scer\GAL4GMR.PF (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency) leads to defects in the organization of cone cells and primary cells as well as in inter-ommatidial cells. Most inter-ommatidial cells sort into a single file and ommatidial spacing is not significantly affected. Less than 4% of ommatidia are found in direct contact with each other.

In the developing eyes of hbsIR.Scer\UAS; Scer\GAL4GMR.PF animals at 24 hours APF, ommatidial cores are largely normal, but inter-ommatidial precursor cells (IPCs) fail to sort into a single line as in wild-type. These IPCs have an abnormally relaxed morphology - failing to maximize their contacts with developing primary pigment cells or to minimize their contacts with other IPCs. In addition, inter-ommatidial bristle precursors are often missing or misplaced. The resulting adults have rough eyes dues to disorganized rows of ommatidia.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference

Simultaneous disruption of sns and hbs through co-expression of snsdsRNA.Scer\UAS and hbsIR.Scer\UAS leads to the direct contact of 27.2% of ommatidia and complete failure of inter-ommatidial cells to sort into single file. These phenotypes represent a strong enhancement of either knockdown alone.

Reduction of both hbs and kirre expression through kirredsRNA.Scer\UAS and hbsIR.Scer\UAS expression under the control of Scer\GAL4GMR.PF enhances slightly the patterning defects seen in kirre down-regulation. Depletion occasionally leads to inter-ommatidial cells occurring in multiple rows between ommatidia. In each case, ommatidial spacing is nonetheless nearly indistinguishable from single knock-down of hbs alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
hbsIR.Scer\UAS
hbsdsRNA.Scer\UAS.cBa
hbsdsRNA.UAS.cBa
Name Synonyms
Secondary FlyBase IDs
    References (4)