Simultaneous disruption of sns and hbs through co-expression of snsdsRNA.Scer\UAS and hbsIR.Scer\UAS leads to the direct contact of 27.2% of ommatidia and complete failure of inter-ommatidial cells to sort into single file. These phenotypes represent a strong enhancement of either knockdown alone.
Reduction of both hbs and kirre expression through kirredsRNA.Scer\UAS and hbsIR.Scer\UAS expression under the control of Scer\GAL4GMR.PF enhances slightly the patterning defects seen in kirre down-regulation. Depletion occasionally leads to inter-ommatidial cells occurring in multiple rows between ommatidia. In each case, ommatidial spacing is nonetheless nearly indistinguishable from single knock-down of hbs alone.