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General Information
Symbol
Dmel\UbqndsRNA.Ex2.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0241241
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of inverted repeat sequences corresponding to exon 2 of Ubqn (a 545bp fragment).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Male flies silencing Ubqn through expression of UbqndsRNA.Ex2.Scer\UAS in neurons under the control of Scer\GAL4elav-C155 suffer premature death. The flies are born normally, but life-span begins to decline shortly after eclosion and significantly declines by the time the flies are 30 days old. At 50 days, over 70% of control flies are still alive, whereas over 80% of UbqndsRNA.Ex2.Scer\UAS;Scer\GAL4elav-C155 flies are already dead. In contrast, no significant difference in life-span is found in mutant females compared to wild-type.

UbqndsRNA.Ex2.Scer\UAS;Scer\GAL4elav-C155 male flies exhibit severe widespread neurodegeneration compared with wild-type flies. In addition, multilamellar structures are found throughout the brain, and the neuropil contains multiple membrane-bound vacuoles.

Silencing of Ubqn by expression of UbqndsRNA.Ex2.Scer\UAS under the control of Scer\GAL4sd-SG29.1 in wing discs results in three types of phenotypes: (i) loss of the partial L4, L5, entire anterior cross vein and posterior cross vein; (ii) a thickened L3 vein, particularly in the distal portion of the margin area of the wing; (iii) notches in the wing margin. These phenotypes show variable penetrance in male and female flies. Compared with female flies, male UbqndsRNA.Ex2.Scer\UAS;Scer\GAL4elav-C155 flies are semi-lethal. All the male flies and approximately 5% of female flies exhibit all three severe phenotypes. The majority of the female flies (95%) exhibit only a weak phenotype as evidenced by loss of the posterior cross vein and anterior cross vein.

Silencing of Ubqn by expression of UbqndsRNA.Ex2.Scer\UAS in the wing disc under the control of Scer\GAL4e22c leads to loss of the entire posterior cross vein, partial loss of the anterior cross vein, and L5 veins and weakened distal portion of L4 and L3-4 M veins in the posterior portion of the wing.

Silencing of Ubqn by expression of UbqndsRNA.Ex2.Scer\UAS in the wing disc under the control of Scer\GAL4ptc-559.1 results in loss of the entire anterior cross vein, narrowing of the intervein sector between L3 and L4 veins in all flies, compared with wild-type controls.

Silencing of Ubqn by expression of UbqndsRNA.Ex2.Scer\UAS in the developing eye disc under the control of Scer\GAL4GMR.PF does not lead to any detectable eye abnormalities.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of UbqndsRNA.Ex2.Scer\UAS by Scer\GAL4e22c in a heterozygous rhove-1 background leads to a normal anterior cross vein and partially restored posterior cross vein and L5 vein, while the distal portion of L4 and L3-4 M veins corresponding to the posterior portion of the wing remains weakened, compared with UbqndsRNA.Ex2.Scer\UAS; Scer\GAL4e22c single mutants.

Silencing of Ubqn through co-expression of UbqndsRNA.Ex2.Scer\UAS with Psn+14.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF enhances the Psn+14.Scer\UAS-induced rough eye phenotype, leading to an even smaller and rougher eye than that observed with Psn+14.Scer\UAS expression alone. Double mutants exhibit a loss of eye pigmentation, suggesting the loss of pigment cells. RNAi silencing of Ubqn in addition to Psn+14.Scer\UAS overexpression results in a much more severe degenerative phenotype than Psn+14.Scer\UAS single mutants, as evidenced by numerous visible holes (2-5¤ům in diameter) on a small, rough eye. In addition, the ommatidia are very irregular and mostly fused with very few bristles.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
UbqndsRNA.Ex2.Scer\UAS
UbqndsRNA.Ex2.UAS
Name Synonyms
Secondary FlyBase IDs
    References (1)