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General Information
Symbol
Dmel\Rbf15aΔ
Species
D. melanogaster
Name
FlyBase ID
FBal0243347
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P-element insertion P{CaSpeR}Rbf15a.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Somatic clones of Rbf15aΔ/Rbf15aΔ cells display elevated levels of apoptosis (detected by Caspase-3 staining) in the third instar larval eye disc if the clones are located near the morphogenetic furrow (MF); in clones posterior to MF or in somatic clones in the wing disc the levels of cell death are very low.

Rbf15aΔ mutant eye clones are smaller in size than wild type controls.

Rbf15aΔ mutant eye disc clones show increased apoptosis just anterior to the morphogenetic furrow.

48 hours after puparium formation, Rbf15aΔ mutant eye disc ommatidia contain between three and five cone cells per cluster, compared with four per cluster in wildtype ommatidia.

No multiple photoreceptor R8 cells are observed within a single ommatidial cluster in Rbf15aΔ third instar larval eye disc clones.

Rbf15aΔ somatic clones in the eye differentiate into photoreceptors at a comparable rate to surrounding control tissue during third instar larval development.

Rbf15aΔ mutant clones show significant amounts of apoptosis near the morphogenetic furrow. Cells far anterior or posterior to the furrow do not exhibit increased cell death.

Rbf15aΔ somatic clones show increased cell proliferation in the posterior region of the wing disc.

Rbf15aΔ mutant clones occupy a smaller area in the adult eye compared to control wildtype clones. Eye disc clones spanning the morphogenetic furrow show increased levels of cell death, and nuclear pyknosis is visible in these cells.

Rbf15aΔ/Rbf120-3-3 mutants eye discs show elevated levels of cell death and pyknotic nuclei.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
Reference

Rbf15aΔ, gig64 has increased cell death phenotype, suppressible by Dronc1

Rbf15aΔ, gig64 has increased cell death phenotype, suppressible by E2f1i2

Rbf15aΔ, gig64 has increased cell death phenotype, suppressible by S6kl-1

Rbf15aΔ, gig192 has increased cell death phenotype, suppressible by Dronc1

Rbf15aΔ, gig192 has increased cell death phenotype, suppressible by E2f1i2

Rbf15aΔ, gig192 has increased cell death phenotype, suppressible by S6kl-1

NOT suppressed by
Enhancer of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Rbf15aΔ has eye | somatic clone phenotype, enhanceable by Axn127

Rbf15aΔ has eye disc | somatic clone phenotype, enhanceable by Axn127

Rbf15aΔ has eye disc | somatic clone phenotype, enhanceable by AxnE77

NOT Enhanced by
Suppressed by
Statement
Reference

Rbf15aΔ, gig64 has eye | somatic clone phenotype, suppressible by E2f1i2

Rbf15aΔ, gig64 has eye | somatic clone phenotype, suppressible by S6kl-1

Rbf15aΔ, gig192 has eye | somatic clone phenotype, suppressible by E2f1i2

Rbf15aΔ, gig192 has eye | somatic clone phenotype, suppressible by S6kl-1

Rbf15aΔ, rno3 has eye disc | somatic clone phenotype, suppressible by Dronc1

NOT suppressed by
Enhancer of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Somatic clones of cells double homozygous for Rbf15aΔ plus any of the following: Stam19, Stam2L3297, Hrs4-19-3, Hrs5-14-3 or HrsD28 display significantly higher levels of apoptosis in third instar larval wing and eye discs, even in clones posterior to the eye disc morphogenetic furrow, compared to clones homozygous for any of the above alleles alone.

In the adult eye, the double homozygous Rbf15aΔ;Stam19 as well as Rbf15aΔ;Hrs4-19-3 clones are very small and difficult to recover compared to each of the single homozygous clones.

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf15aΔ;Stam19 double homozygous somatic in third instar larval eye disc is completely abolished when the clones are created in either hidw138 or E2f1i2/E2f1rM729 mutant background.

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf15aΔ;Stam19 double homozygous somatic MARCM clones in third instar larval eye disc are strongly suppressed by expression of either of the following: Ras85DV12.Scer\UAS, Egfr2.A887T.Scer\UAS or spis.Scer\UAS under the control of the Scer\GAL4Act.PU driver in the mutant clones. The apoptotic signal is however not suppressed by expression of rhoScer\UAS.cdCa.

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf15aΔ homozygous somatic MARCM clones near the morphogenetic furrow in third instar larval eye disc is strongly suppressed by expression of rhoScer\UAS.cdCa under the control of the Scer\GAL4Act.PU driver in the mutant clones.

Axn127 enhances the decrease in clone size seen in Rbf15aΔ mutant adult eyes. The double mutant clones are very small or undetectable. The increased apoptosis seen anterior to the morphogenetic furrow in Rbf15aΔ mutant eye disc clones is also enhanced in Rbf15aΔ Axn127 double mutant clones.

AxnEY10228 enhances the increase in apoptosis anterior to the morphogenetic furrow seen in Rbf15aΔ mutant eye disc clones. Apoptosis is seen both anterior and posterior to the morphogenetic furrow in the double mutants.

AxnE77 enhances the increase in apoptosis anterior to the morphogenetic furrow seen in Rbf15aΔ mutant eye disc clones. Apoptosis is seen both anterior and posterior to the morphogenetic furrow in the double mutants.

AxnS044230 enhances the increase in apoptosis anterior to the morphogenetic furrow seen in Rbf15aΔ mutant eye disc clones. Apoptosis is seen both anterior and posterior to the morphogenetic furrow in the double mutants. Inhibiting wg signalling through expression of panΔN.Scer\UAS under the control of a GAL4 driver suppresses this enhancement.

A ApcQ8 Df(3R)Apc2-79 double mutant background enhances the increase in apoptosis anterior to the morphogenetic furrow seen in Rbf15aΔ mutant eye disc clones.

Rheb4L1 significantly decreases the increase in apoptosis anterior to the morphogenetic furrow seen in AxnS044230 Rbf15aΔ mutant eye disc clones.

Expression of raptorHMS00124 under the control of a GAL4 driver significantly decreases the increase in apoptosis anterior to the morphogenetic furrow seen in AxnS044230 Rbf15aΔ mutant eye disc clones.

Rbf15aΔ enhances the reduction in ATP/ADP ratio seen in eye discs with AxnS044230 mutant clones (induced in a minute background). This enhancement is suppressed to the levels seen in AxnS044230 single mutants by Rheb4L1.

lkb1X5 significantly enhances the increase in apoptosis anterior to the morphogenetic furrow seen in AxnS044230 Rbf15aΔ mutant eye disc clones.

hidw138 largely blocks the increase in apoptosis anterior to the morphogenetic furrow seen in AxnS044230 Rbf15aΔ mutant eye disc clones.

gig64/Rbf15aΔ mutant eye clones are smaller than gig64 mutant eye clones. Adult eyes with gig64/Rbf15aΔ double mutant cones are smaller and display a rough appearance, compared to wild-type eyes. This correlates with an increase in apoptosis in both anterior and posterior (to the morphogenic furrow) clones in double mutant eye discs. This synergistic induction of cell death is also seen in the wing disc.

The presence of a W05014 background significantly decreases gig192/Rbf15aΔ induced cell death. Some cell death is still observed, particularly in the anterior of the eye disc.

The presence of a Nc1 background significantly decreases gig192/Rbf15aΔ induced cell death in the posterior (the differentiating part of the eye disc). However Nc1 has little effect on cell death of gig192/Rbf15aΔ clones in the anterior, the proliferating part of the eye disc. These triple mutants exhibit an increase in mutant tissue in the eye as well as their overall size.

The presence of an E2fi2 background significantly decreases gig192/Rbf15aΔ induced cell death in the eye. In addition, much larger gig192/Rbf15aΔ double mutant clones are observed in adult eyes.

The presence of a S6kl-1 background significantly decreases gig192/Rbf15aΔ induced cell death in the eye. In addition, much larger gig192/Rbf15aΔ double mutant clones are observed in adult eyes.

gig192/Rbf15aΔ mutant eye clones are smaller than gig192 mutant eye clones. Adult eyes with gig192/Rbf15aΔ double mutant cones are smaller and display a rough appearance, compared to wild-type eyes. This correlates with an increase in apoptosis in both anterior and posterior (to the morphogenic furrow) clones in double mutant eye discs. This synergistic induction of cell death is also seen in the wing disc.

The presence of a W05014 background significantly decreases gig192/Rbf15aΔ induced cell death. Some cell death is still observed, particularly in the anterior of the eye disc.

The presence of a Nc1 background significantly decreases gig192/Rbf15aΔ induced cell death in the posterior (the differentiating part of the eye disc). However Nc1 has little effect on cell death of gig192/Rbf15aΔ clones in the anterior, the proliferating part of the eye disc. These triple mutants exhibit an increase in mutant tissue in the eye as well as their overall size.

The presence of an E2fi2 background significantly decreases gig192/Rbf15aΔ induced cell death in the eye. In addition, much larger gig192/Rbf15aΔ double mutant clones are observed in adult eyes.

The presence of a S6kl-1 background significantly decreases gig192/Rbf15aΔ induced cell death in the eye. In addition, much larger gig192/Rbf15aΔ double mutant clones are observed in adult eyes.

Somatic eye clones doubly mutant for Rbf15aΔ and either rno1, rno3 or rnoK develop shiny eye differentiation defects in the adult eye. Pupal eye discs are largely devoid of bristle cells, and adult eyes lack bristles.

48 hours after puparium formation Rbf15aΔ; rno3 mutant eye disc ommatidia contain a variable number of cone cells per cluster. Some contain almost twice the number of normal cone cells, and ommatidial arrangement is very disorganised compared to control tissue.

Multiple photoreceptor R8 cells are observed within the same ommatidial cluster in roughly 20% of third instar larval eye disc clones mutant for Rbf15aΔ and either rno1 or rno3.

The significant amounts of apoptosis observed near the morphogenetic furrow in Rbf15aΔ mutant clones is not enhanced by rno3. However, Rbf15aΔ; rno3 mutant clones carrying an additional mutation in Nc1 show significantly decreased levels of apoptosis.

The shiny-eye phenotype observed in rnoK, Rbf15aΔ mutant somatic clones is not enhanced by Nc1.

Rbf15aΔ; rno3 mutant clones show increased cell proliferation in the posterior region of the wing disc, similar to discs mutant only for Rbf15aΔ.

The multiple-R8 phenotype seen in rno1, Rbf15aΔ eye somatic mutant clones is not significantly affected by Egfrf2/+, but is significantly increased in a Dl05151/+ background.

Expression of DlScer\UAS.cHa under the control of Scer\GAL4h-H10 significantly suppresses the multiple photoreceptor-R8 phenotype in rno3; Rbf15aΔ somatic clones in the eye.

The shiny-eye and multiple photoreceptor-R8 phenotype seen in rno1, Rbf15aΔ eye somatic mutant clones is significantly suppressed in a E2fi2/E2frM729 mutant background.

In third instar larval eye discs, cells from somatic clones mutant for both rno1 and Rbf15aΔ show significant and enhanced delays in photoreceptor differentiation compared to controls.

Rbf15aΔ; Ww138 double mutant clones occupy a dramatically increased area of the eye compared to Rbf15aΔ single mutant clones. The morphology of the eye is relatively normal with occasional extra interommatidial bristles. No cell death or nuclear pyknosis is observed in eye disc clones.

Rbf15aΔ; W05014 double mutant clones occupy a dramatically increased area of the eye compared to Rbf15aΔ single mutant clones. The morphology of the eye is relatively normal with occasional extra interommatidial bristles. No cell death or nuclear pyknosis is observed in eye disc clones.

A significant increase in the level of cell death is observed in Rbf15aΔ;banEP3622 double mutant eye disc clones compared to Rbf15aΔ or banEP3622 single clones.

Xenogenetic Interactions
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Complementation and Rescue Data
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Synonyms and Secondary IDs (2)
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    References (6)