FlyBase Allele Report: Dmel\Ras85D[KP]

General Information

Symbol

Dmel\Ras85D^KP

Species

D. melanogaster

Name

FlyBase ID

FBal0244615

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

P{KP}Ras85D^KP

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Wu et al., 2009)

Progenitor genotype

Associated Insertion(s)

P{KP}Ras85D^KP

Cytology

Description

Insertion of a 1165 bp fragment of a P{KP} element in the second exon of Ras85D, located 88 bp upstream of the start codon.

(Wu et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:9,512,379..9,512,379

(Wu et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size

(Wu et al., 2009)

decreased cell size

(Wu et al., 2009)

female semi-sterile

(Wu et al., 2009)

increased cell death | third instar larval stage

(Wu et al., 2009)

partially lethal - majority die

(Wu et al., 2009)

some die during pupal stage

(Wu et al., 2009)

Phenotype Manifest In

eye

(Wu et al., 2009)

eye disc | third instar larval stage

(Wu et al., 2009)

ommatidium

(Wu et al., 2009)

photoreceptor neuron

(Wu et al., 2009)

wing

(Wu et al., 2009)

Detailed Description

Statement

Reference

Ras85D^KP and wild-type larvae are comparable in body size. However, mutant adults show significantly reduced body weight. Homozygous mutants show a 23.5% reduction in wing size, and decreased wing cell size.

Ras85D^KP mutant adults have a rough-eye phenotype and the eye is reduced in size. Neuronal differentiation in third instar larval eye discs is observed, however adult eyes show largely disorganised ommatidia. The full complement of photoreceptors are seen, but many ommatidia contain fewer photoreceptor cells. There is substantially increased levels of cell death in third instar larval eye discs.

(Wu et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ras85D^KP has increased cell death phenotype, suppressible | partially by hid⁰⁵⁰¹⁴/Df(3L)H99

(Wu et al., 2009)

Ras85D^KP has visible phenotype, suppressible | partially by hid⁰⁵⁰¹⁴/Df(3L)H99

(Wu et al., 2009)

Ras85D^KP has visible phenotype, suppressible | partially by Traf4^RNAi.UAS/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has visible phenotype, suppressible | partially by Tak1^NIG.1388R/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has visible phenotype, suppressible | partially by hep^NIG.4353R/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has visible phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has visible phenotype, suppressible | partially by egr^RNAi.UAS/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has visible phenotype, suppressible | partially by wgn^RNAi.UAS/Scer\GAL4^GMR.PF

(Wu et al., 2009)

hid⁰⁵⁰¹⁴/Df(3L)H99, Ras85D^KP has visible phenotype, suppressible by hep^NIG.4353R/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has visible phenotype, suppressible | partially by BacA\p35^GMR.PH

(Wu et al., 2009)

BacA\p35^GMR.PH, Ras85D^KP has visible phenotype, suppressible by Traf4^RNAi.UAS/Scer\GAL4^GMR.PF

(Wu et al., 2009)

BacA\p35^GMR.PH, Ras85D^KP has visible phenotype, suppressible by hep^NIG.4353R/Scer\GAL4^GMR.PF

(Wu et al., 2009)

BacA\p35^GMR.PH, Ras85D^KP has increased cell death phenotype, suppressible by hep^NIG.4353R/Scer\GAL4^GMR.PF

(Wu et al., 2009)

NOT suppressed by

Statement

Reference

Ras85D^KP has visible phenotype, non-suppressible by Scer\GAL4^GMR.PF/Pi3K92E^UAS.Tag:MYC

(Wu et al., 2009)

Ras85D^KP has visible phenotype, non-suppressible by Akt^UAS.Tag:HA/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has visible phenotype, non-suppressible by Buffy^UAS.cQa/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ras85D^KP has eye phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has eye phenotype, suppressible | partially by egr^RNAi.UAS/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has eye phenotype, suppressible | partially by wgn^RNAi.UAS/Scer\GAL4^GMR.PF

(Wu et al., 2009)

hid⁰⁵⁰¹⁴/Df(3L)H99, Ras85D^KP has eye phenotype, suppressible by hep^NIG.4353R/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has eye phenotype, suppressible | partially by BacA\p35^GMR.PH

(Wu et al., 2009)

BacA\p35^GMR.PH, Ras85D^KP has eye phenotype, suppressible by Traf4^RNAi.UAS/Scer\GAL4^GMR.PF

(Wu et al., 2009)

BacA\p35^GMR.PH, Ras85D^KP has eye phenotype, suppressible by hep^NIG.4353R/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has eye phenotype, suppressible | partially by hid⁰⁵⁰¹⁴/Df(3L)H99

(Wu et al., 2009)

Ras85D^KP has eye phenotype, suppressible | partially by Traf4^RNAi.UAS/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has eye phenotype, suppressible | partially by Tak1^NIG.1388R/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has eye phenotype, suppressible | partially by hep^NIG.4353R/Scer\GAL4^GMR.PF

(Wu et al., 2009)

NOT suppressed by

Statement

Reference

Ras85D^KP has eye phenotype, non-suppressible by Scer\GAL4^GMR.PF/Pi3K92E^UAS.Tag:MYC

(Wu et al., 2009)

Ras85D^KP has eye phenotype, non-suppressible by Akt^UAS.Tag:HA/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Ras85D^KP has eye phenotype, non-suppressible by Buffy^UAS.cQa/Scer\GAL4^GMR.PF

(Wu et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Df(3L)H99/W⁰⁵⁰¹⁴ partially suppresses the cell death phenotype of Ras85D^KP mutant flies.

The eye phenotype in Ras85D^KP mutant flies is not suppressed by ectopic expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Akt1^{Scer\UAS.T:Ivir\HA1} or Buffy^Scer\UAS.cQa under the control of Scer\GAL4^GMR.PF.

The eye phenotype in Ras85D^KP mutant flies is partially suppressed by ectopic expression of Traf4^{dsRNA.Scer\UAS}, Tak1^{dsRNA.Scer\UAS}, hep^{dsRNA.Scer\UAS}, bsk^DN.Scer\UAS, egr^IR.Scer\UAS, or wgn^{dsRNA.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF.

Expression of hep^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^GMR.PF) in flies carrying Df(3L)H99/W⁰⁵⁰¹⁴ in a Ras85D^KP mutant background strongly suppresses the eye phenotype.

(Wu et al., 2009)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^GMR.PH in a Ras85D^KP mutant background only partially suppresses increased cell death.

Expression of Traf4^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^GMR.PF) and BacA\p35^GMR.PH in a Ras85D^KP mutant background strongly suppresses the eye phenotype.

Expression of hep^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^GMR.PF) and BacA\p35^GMR.PH in a Ras85D^KP mutant background strongly suppresses the eye phenotype, and much reduced levels of cell death are observed.

(Wu et al., 2009)

Complementation and Rescue Data

Fails to complement

Ras85D^e2F

(Wu et al., 2009)

Ras85D^e1B

(Wu et al., 2009)

Ras85D^ΔC17b

(Wu et al., 2009)

Rescued by

Ras85D^KP is rescued by Scer\GAL4^Act.PU/Ras85D^UAS.cKa

(Wu et al., 2009)

Comments

Expression of Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^Act in Ras85D^KP mutant animals fully rescues the mutant eye phenotype.

(Wu et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ras85D^KP

ras^KP

(Wu et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (1)

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