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General Information
Symbol
Dmel\cathD1
Species
D. melanogaster
Name
FlyBase ID
FBal0246638
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion P{EP}cathDEP2151 results in a 916 bp deletion in the 5' end of cathD, extending from 77 bp upstream from the start codon of cathD to nucleotide 847 of the coding sequence.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

cathD1 homozygotes or heterozygotes do not exhibit a significant increase in the proportion of hyperplastic testes, as compared to controls.

cathD1 mutant males exhibit ~60% less spermatogonial cyst death compared to controls.

cathD1 homozygous adults exhibit an apparently normal lifespan and their brains exhibit apparently normal levels of apoptosis (as visualized with TUNEL), DNA replication (as visualized with PCNA) and vacuolization, as compared to controls.

33% of cathD1 stage 14 egg chambers show persisting nurse cell nuclei, compared to 7% in controls. 2% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.

Forty-five day old cathD1 mutant retina exhibit slight vacuolar changes (due to eye degeneration) compared to wild-type controls.

Significant numbers of apoptotic nuclei are observed in the brains of 45-day old cathD1/Df(2R)CA53 or cathD1 homozygous flies, compared to rare apoptotic nuclei observed in control brains. A normal number of apoptotic cells are observed in 1, 15 and 30-day old mutant flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

cathD1 has eye phenotype, enhanceable by schlank[+]/schlankG0061

cathD1 has eye phenotype, enhanceable by CtsBPG148/CG10992[+]

cathD1 has eye phenotype, enhanceable by shi[+]/shi1

cathD1 has eye phenotype, enhanceable by Hsc70-4L3929/Hsc70-4[+]

cathD1 has eye phenotype, enhanceable by Trxr-1G0481/Trxr-1[+]

NOT Enhanced by
Statement
Reference

cathD1 has eye phenotype, non-enhanceable by Hsc70-3G0102/Hsc70-3[+]

cathD1 has eye phenotype, non-enhanceable by SNF4Agamma[+]/SNF4Aγloe

cathD1 has eye phenotype, non-enhanceable by Sap-r[+]/Sap-re01294

cathD1 has eye phenotype, non-enhanceable by Sap-re01294

cathD1 has eye phenotype, non-enhanceable by Scer\GAL4elav-C155/CtsBUAS.cKa

cathD1 has eye phenotype, non-enhanceable by sws[+]/swsd07605

cathD1 has eye phenotype, non-enhanceable by CG17841PL94/CG17841[+]

cathD1 has eye phenotype, non-enhanceable by Sod1n1/Sod[+]

cathD1 has eye phenotype, non-enhanceable by Scer\GAL4elav-C155/TorTED.UAS

Enhancer of
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

cathD1/cathD1, p53E8/+ adults exhibit a significant proportion of hyperplastic testes, as compared to cathD1/cathD1 adults, p53E8/+ adults, or wild-type controls.

Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 enhances by 2-10-fold the level of vacuolar change in cathD1 mutant retina.

A CtsB1PG148 mutant background enhances by 2-10-fold the level of vacuolar change in cathD1 mutant retina.

A shi1 mutant background enhances by more than 10-fold the level of vacuolar change in cathD1 mutant retina.

A Hsc70-4L3929 mutant background enhances by more than 10-fold the level of vacuolar change in cathD1 mutant retina.

Expression of HmgcrScer\UAS.cvDa under the control of Scer\GAL4elav-C155 enhances by more than 10-fold the level of vacuolar change in cathD1 mutant retina.

A Trxr-1G0481 mutant background enhances by more than 10-fold the level of vacuolar change in cathD1 mutant retina.

Xenogenetic Interactions
Statement
Reference

A cathD1 homozygous background further reduces the lifespan of Hsap\MAPTR406W.Scer\UAS (Scer\GAL4elav-C155) flies by almost 50%.

Flies expressing Hsap\MAPTR406W.Scer\UAS neuronally (under the control of Scer\GAL4elav-C155) in a cathD1 null background exhibit an almost 3-fold increase in apoptotic cell death (as visualized with TUNEL). A similar effect is seen on neurodegeneration, with an increase in vacuolar degeneration in aged double mutant flies. An increase in PCNA staining is also seen in these double mutants (and not the respective single mutants), indicating that cathD modulation of Hsap\MAPT toxicity occurs via abnormal cell-cycle re-entry.

Removing cathD from mutant Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpressing flies (under the control of Scer\GAL4elav-C155 in a cathD1 background) has no effect on Kenyon cell loss.

Complementation and Rescue Data
Comments

The increase in retinal vacuoles due to eye degeneration in 45 day old cathD1 mutants is rescued upon expression of cathDScer\UAS.cKa under the control of Scer\GAL4elav-C155.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (9)