FlyBase Allele Report: Dmel\cathD[1]

General Information

Symbol

Dmel\cathD¹

Species

D. melanogaster

Name

FlyBase ID

FBal0246638

Feature type

allele

Associated gene

Dmel\cathD

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Myllykangas et al., 2005)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{EP}cathD^EP2151

(Myllykangas et al., 2005)

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of the progenitor insertion P{EP}cathD^EP2151 results in a 916 bp deletion in the 5' end of cathD, extending from 77 bp upstream from the start codon of cathD to nucleotide 847 of the coding sequence.

(Myllykangas et al., 2005)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neuronal ceroid lipofuscinosis

CEA

(Myllykangas et al., 2005)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Khurana et al., 2010)

model of neuronal ceroid lipofuscinosis

is exacerbated by Hmgcr^UAS.cvDa

(Kuronen et al., 2009)

is exacerbated by Hsc70-4^L3929

(Kuronen et al., 2009)

is exacerbated by Tor^{WT.UAS.Tag:FLAG}

(Kuronen et al., 2009)

is exacerbated by CtsB^PG148

(Kuronen et al., 2009)

is exacerbated by schlank^G0061

(Kuronen et al., 2009)

is exacerbated by shi¹

(Kuronen et al., 2009)

is exacerbated by Trxr-1^G0481

(Kuronen et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death | oogenesis stage S14

(Bass et al., 2009)

decreased cell death

(Yacobi-Sharon et al., 2013)

fertile

(Myllykangas et al., 2005)

increased cell death | conditional

(Myllykangas et al., 2005)

increased cell death | conditional (with Df(2R)CA53)

(Myllykangas et al., 2005)

viable

(Myllykangas et al., 2005)

Phenotype Manifest In

adult brain | conditional

(Myllykangas et al., 2005)

adult brain | conditional (with Df(2R)CA53)

(Myllykangas et al., 2005)

egg chamber | oogenesis stage S14

(Bass et al., 2009)

eye

(Kuronen et al., 2009)

male germline cell

(Yacobi-Sharon et al., 2013)

nurse cell | oogenesis stage S14

(Bass et al., 2009)

spermatogonial cyst

(Yacobi-Sharon et al., 2013)

Detailed Description

Statement

Reference

cathD¹ homozygotes or heterozygotes do not exhibit a significant increase in the proportion of hyperplastic testes, as compared to controls.

(Napoletano et al., 2017)

cathD¹ mutant males exhibit ~60% less spermatogonial cyst death compared to controls.

(Yacobi-Sharon et al., 2013)

cathD¹ homozygous adults exhibit an apparently normal lifespan and their brains exhibit apparently normal levels of apoptosis (as visualized with TUNEL), DNA replication (as visualized with PCNA) and vacuolization, as compared to controls.

(Khurana et al., 2010)

33% of cathD¹ stage 14 egg chambers show persisting nurse cell nuclei, compared to 7% in controls. 2% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.

(Bass et al., 2009)

Forty-five day old cathD¹ mutant retina exhibit slight vacuolar changes (due to eye degeneration) compared to wild-type controls.

(Kuronen et al., 2009)

Significant numbers of apoptotic nuclei are observed in the brains of 45-day old cathD¹/Df(2R)CA53 or cathD¹ homozygous flies, compared to rare apoptotic nuclei observed in control brains. A normal number of apoptotic cells are observed in 1, 15 and 30-day old mutant flies.

(Myllykangas et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

cathD¹ is an enhancer of short lived phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav-C155

(Khurana et al., 2010)

cathD¹ is an enhancer of increased cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav-C155

(Khurana et al., 2010)

cathD¹/cathD¹ is an enhancer of increased occurrence of cell division phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav-C155

(Khurana et al., 2010)

Other

Statement

Reference

cathD¹, p53[+]/p53^E8 has hyperplasia | spermatogenesis phenotype

(Napoletano et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

cathD¹ has eye phenotype, enhanceable by schlank[+]/schlank^G0061

(Kuronen et al., 2009)

cathD¹ has eye phenotype, enhanceable by Scer\GAL4^elav-C155/Tor^{WT.UAS.Tag:FLAG}

(Kuronen et al., 2009)

cathD¹ has eye phenotype, enhanceable by Scer\GAL4^elav-C155/Hmgcr^UAS.cvDa

(Kuronen et al., 2009)

cathD¹ has eye phenotype, enhanceable by CtsB^PG148/CG10992[+]

(Kuronen et al., 2009)

cathD¹ has eye phenotype, enhanceable by shi[+]/shi¹

(Kuronen et al., 2009)

cathD¹ has eye phenotype, enhanceable by Hsc70-4^L3929/Hsc70-4[+]

(Kuronen et al., 2009)

cathD¹ has eye phenotype, enhanceable by Trxr-1^G0481/Trxr-1[+]

(Kuronen et al., 2009)

NOT Enhanced by

Statement

Reference

cathD¹ has eye phenotype, non-enhanceable by Hsc70-3^G0102/Hsc70-3[+]

(Kuronen et al., 2009)

cathD¹ has eye phenotype, non-enhanceable by Scer\GAL4^elav-C155/CDase^UAS.cAa

(Kuronen et al., 2009)

cathD¹ has eye phenotype, non-enhanceable by SNF4Agamma[+]/SNF4Aγ^loe

(Kuronen et al., 2009)

cathD¹ has eye phenotype, non-enhanceable by Scer\GAL4^elav-C155/SNF4Aγ^loeI.UAS

(Kuronen et al., 2009)

cathD¹ has eye phenotype, non-enhanceable by Sap-r[+]/Sap-r^e01294

(Kuronen et al., 2009)

cathD¹ has eye phenotype, non-enhanceable by Sap-r^e01294

(Kuronen et al., 2009)

cathD¹ has eye phenotype, non-enhanceable by Scer\GAL4^elav-C155/CtsB^UAS.cKa

(Kuronen et al., 2009)

cathD¹ has eye phenotype, non-enhanceable by sws[+]/sws^d07605

(Kuronen et al., 2009)

cathD¹ has eye phenotype, non-enhanceable by CG17841^PL94/CG17841[+]

(Kuronen et al., 2009)

cathD¹ has eye phenotype, non-enhanceable by Sod1ⁿ¹/Sod[+]

(Kuronen et al., 2009)

cathD¹ has eye phenotype, non-enhanceable by Scer\GAL4^elav-C155/Tor^TED.UAS

(Kuronen et al., 2009)

Enhancer of

Statement

Reference

cathD¹/cathD¹ is an enhancer of adult brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav-C155

(Khurana et al., 2010)

cathD¹/cathD¹ is an enhancer of neuron | adult stage phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav-C155

(Khurana et al., 2010)

NOT Enhancer of

Statement

Reference

cathD¹ is a non-enhancer of Kenyon cell phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^elav-C155

(Khurana et al., 2010)

NOT Suppressor of

Statement

Reference

cathD¹ is a non-suppressor of Kenyon cell phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^elav-C155

(Khurana et al., 2010)

Other

Statement

Reference

cathD¹, p53[+]/p53^E8 has testis | adult stage phenotype

(Napoletano et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

cathD¹/cathD¹, p53^E8/+ adults exhibit a significant proportion of hyperplastic testes, as compared to cathD¹/cathD¹ adults, p53^E8/+ adults, or wild-type controls.

(Napoletano et al., 2017)

Expression of Tor^{WT.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 enhances by 2-10-fold the level of vacuolar change in cathD¹ mutant retina.

A CtsB1^PG148 mutant background enhances by 2-10-fold the level of vacuolar change in cathD¹ mutant retina.

A shi¹ mutant background enhances by more than 10-fold the level of vacuolar change in cathD¹ mutant retina.

A Hsc70-4^L3929 mutant background enhances by more than 10-fold the level of vacuolar change in cathD¹ mutant retina.

Expression of Hmgcr^{Scer\UAS.cvDa} under the control of Scer\GAL4^elav-C155 enhances by more than 10-fold the level of vacuolar change in cathD¹ mutant retina.

A Trxr-1^G0481 mutant background enhances by more than 10-fold the level of vacuolar change in cathD¹ mutant retina.

(Kuronen et al., 2009)

Xenogenetic Interactions

Statement

Reference

A cathD¹ homozygous background further reduces the lifespan of Hsap\MAPT^{R406W.Scer\UAS} (Scer\GAL4^elav-C155) flies by almost 50%.

Flies expressing Hsap\MAPT^{R406W.Scer\UAS} neuronally (under the control of Scer\GAL4^elav-C155) in a cathD¹ null background exhibit an almost 3-fold increase in apoptotic cell death (as visualized with TUNEL). A similar effect is seen on neurodegeneration, with an increase in vacuolar degeneration in aged double mutant flies. An increase in PCNA staining is also seen in these double mutants (and not the respective single mutants), indicating that cathD modulation of Hsap\MAPT toxicity occurs via abnormal cell-cycle re-entry.

Removing cathD from mutant Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} overexpressing flies (under the control of Scer\GAL4^elav-C155 in a cathD¹ background) has no effect on Kenyon cell loss.

(Khurana et al., 2010)

Complementation and Rescue Data

Rescued by

cathD¹ is rescued by Scer\GAL4^elav-C155/cathD^UAS.cKa

(Kuronen et al., 2009)

Comments

The increase in retinal vacuoles due to eye degeneration in 45 day old cathD¹ mutants is rescued upon expression of cathD^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155.

(Kuronen et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CathD¹

(Ling and Salvaterra, 2011)

cathD¹

(Jacomin et al., 2021, Tarayrah-Ibraheim et al., 2021, Zhang et al., 2020, Napoletano et al., 2017, Yacobi-Sharon et al., 2013, Luan et al., 2012, Khurana et al., 2010, Bass et al., 2009, Kuronen et al., 2009, Myllykangas et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (11)

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