Imprecise excision of the progenitor insertion P{EP}cathDEP2151 results in a 916 bp deletion in the 5' end of cathD, extending from 77 bp upstream from the start codon of cathD to nucleotide 847 of the coding sequence.
cathD1 homozygotes or heterozygotes do not exhibit a significant increase in the proportion of hyperplastic testes, as compared to controls.
cathD1 mutant males exhibit ~60% less spermatogonial cyst death compared to controls.
cathD1 homozygous adults exhibit an apparently normal lifespan and their brains exhibit apparently normal levels of apoptosis (as visualized with TUNEL), DNA replication (as visualized with PCNA) and vacuolization, as compared to controls.
33% of cathD1 stage 14 egg chambers show persisting nurse cell nuclei, compared to 7% in controls. 2% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.
Forty-five day old cathD1 mutant retina exhibit slight vacuolar changes (due to eye degeneration) compared to wild-type controls.
Significant numbers of apoptotic nuclei are observed in the brains of 45-day old cathD1/Df(2R)CA53 or cathD1 homozygous flies, compared to rare apoptotic nuclei observed in control brains. A normal number of apoptotic cells are observed in 1, 15 and 30-day old mutant flies.
cathD1 is an enhancer of short lived phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4elav-C155
cathD1 is an enhancer of increased cell death phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4elav-C155
cathD1/cathD1 is an enhancer of increased occurrence of cell division phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4elav-C155
cathD1, p53[+]/p53E8 has hyperplasia | spermatogenesis phenotype
cathD1 has eye phenotype, enhanceable by schlank[+]/schlankG0061
cathD1 has eye phenotype, enhanceable by Scer\GAL4elav-C155/TorWT.UAS.Tag:FLAG
cathD1 has eye phenotype, enhanceable by Scer\GAL4elav-C155/HmgcrUAS.cvDa
cathD1 has eye phenotype, enhanceable by Hsc70-4L3929/Hsc70-4[+]
cathD1 has eye phenotype, enhanceable by Trxr-1G0481/Trxr-1[+]
cathD1 has eye phenotype, non-enhanceable by Hsc70-3G0102/Hsc70-3[+]
cathD1 has eye phenotype, non-enhanceable by Scer\GAL4elav-C155/CDaseUAS.cAa
cathD1 has eye phenotype, non-enhanceable by Scer\GAL4elav-C155/SNF4AγloeI.UAS
cathD1 has eye phenotype, non-enhanceable by Sap-r[+]/Sap-re01294
cathD1 has eye phenotype, non-enhanceable by Sap-re01294
cathD1 has eye phenotype, non-enhanceable by Scer\GAL4elav-C155/CtsBUAS.cKa
cathD1 has eye phenotype, non-enhanceable by CG17841PL94/CG17841[+]
cathD1 has eye phenotype, non-enhanceable by Scer\GAL4elav-C155/TorTED.UAS
cathD1/cathD1 is an enhancer of adult brain phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4elav-C155
cathD1/cathD1 is an enhancer of neuron | adult stage phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4elav-C155
cathD1 is a non-enhancer of Kenyon cell phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4elav-C155
cathD1 is a non-suppressor of Kenyon cell phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4elav-C155
cathD1, p53[+]/p53E8 has testis | adult stage phenotype
Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 enhances by 2-10-fold the level of vacuolar change in cathD1 mutant retina.
A CtsB1PG148 mutant background enhances by 2-10-fold the level of vacuolar change in cathD1 mutant retina.
A shi1 mutant background enhances by more than 10-fold the level of vacuolar change in cathD1 mutant retina.
A Hsc70-4L3929 mutant background enhances by more than 10-fold the level of vacuolar change in cathD1 mutant retina.
Expression of HmgcrScer\UAS.cvDa under the control of Scer\GAL4elav-C155 enhances by more than 10-fold the level of vacuolar change in cathD1 mutant retina.
A Trxr-1G0481 mutant background enhances by more than 10-fold the level of vacuolar change in cathD1 mutant retina.
A cathD1 homozygous background further reduces the lifespan of Hsap\MAPTR406W.Scer\UAS (Scer\GAL4elav-C155) flies by almost 50%.
Flies expressing Hsap\MAPTR406W.Scer\UAS neuronally (under the control of Scer\GAL4elav-C155) in a cathD1 null background exhibit an almost 3-fold increase in apoptotic cell death (as visualized with TUNEL). A similar effect is seen on neurodegeneration, with an increase in vacuolar degeneration in aged double mutant flies. An increase in PCNA staining is also seen in these double mutants (and not the respective single mutants), indicating that cathD modulation of Hsap\MAPT toxicity occurs via abnormal cell-cycle re-entry.
Removing cathD from mutant Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpressing flies (under the control of Scer\GAL4elav-C155 in a cathD1 background) has no effect on Kenyon cell loss.
cathD1 is rescued by Scer\GAL4elav-C155/cathDUAS.cKa
The increase in retinal vacuoles due to eye degeneration in 45 day old cathD1 mutants is rescued upon expression of cathDScer\UAS.cKa under the control of Scer\GAL4elav-C155.