FB2025_01 , released February 20, 2025
Allele: Dmel\PlexARNAi.UAS
Open Close
General Information
Symbol
Dmel\PlexARNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0248406
Feature type
allele
Associated gene
Dmel\PlexA
Associated Insertion(s)
Carried in Construct
P{UAS-PlexA.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Yu et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Yu et al., 2010)
Mutagen
in vitro construct
(Yu et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-PlexA.RNAi}
Cytology
Description

UAS regulatory sequences drive expression of 600bp of PlexA sequences in an inverted repeat in a pWIZ vector.

(Yu et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
PlexA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      R1-R6 growth cones scatter around the lamina termination region in third instar larvae where plexAdsRNA.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF, leading to the appearance of a discontinuous termination layer in the lamina.

      (Yu et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Reducing the dose of Sema-1a through the presence of Df(2L)N22-5 significantly enhances the plexAdsRNA.Scer\UAS knockdown phenotype (where expression is controlled by Scer\GAL4GMR.PF). The penetrance of the phenotype is increased from around 20% to approximately 60%, consistent with the idea that plexA and Sema-1a function in the same pathway.

      The severity of the Rho1N19.Scer\UAS axonal-hyperfasciculation phenotype is significantly suppressed by knockdown of plexAdsRNA.Scer\UAS (with both transgenes under the control of Scer\GAL4GMR.PF).

      (Yu et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      PlexARNAi.UAS is rescued by PlexARNAi.UAS/Scer\GAL4GMR.PF

      (Yu et al., 2010)
      Rescues

      PlexARNAi.UAS/Scer\GAL4GMR.PF rescues PlexARNAi.UAS

      (Yu et al., 2010)
      Comments

      Expression of plexAΔcyt.Scer\UAS in R-cells where endogenous plexA is knocked-down by expression of plexAdsRNA.Scer\UAS (both transgenes under the control of Scer\GAL4GMR.PF) largely restores the R1-R6 termination pattern in the lamina.

      (Yu et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      PlexARNAi.UAS
      PlexAdsRNA.Scer\UAS
      PlexAdsRNA.UAS
      plexAdsRNA.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)