FlyBase Allele Report: Dmel\cv-c[1260]

General Information

Symbol

Dmel\cv-c¹²⁶⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0248659

Feature type

allele

Associated gene

Dmel\cv-c

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Sato et al., 2010)

Progenitor genotype

Cytology

Description

Amino acid replacement: R1389term.

(Sato et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:14,399,758..14,399,758 [-]

Nucleotide change:

C14399758T

Amino acid change:

R55term | cv-c-PA; R1389term | cv-c-PC; R1389term | cv-c-PD

Reported amino acid change:

R1389term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Sato et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Sato et al., 2010)

abnormal neuroanatomy | embryonic stage (with cv-c⁰⁶⁹⁵¹)

(Sato et al., 2010)

abnormal neuroanatomy | embryonic stage (with cv-c^f04940)

(Sato et al., 2010)

abnormal neuroanatomy | embryonic stage (with cv-c^f07633)

(Sato et al., 2010)

lethal - all die during embryonic stage

(Sato et al., 2010)

some die during embryonic stage

(Sato et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Cell shape in the leading edge looks less ordered than normal in the mutant embryos during dorsal closure, but the mutant epidermis closes along the dorsal midline with a time course comparable to that of wild-type embryos.

Class I dendritic arborization (da) neurons (ddaD and ddaE) do form primary dendritic branches of normal length. However, both the total length and number of secondary dendritic branches is significantly decreased in the mutant embryos compared to wild type at 20-22 hours after egg laying (AEL), and those secondary branches that have formed in the mutant deviate from the anterior-posterior axis (which the secondary branches tend to elongate along in the wild type). This results in dendrite-free zones being generated between ipsilateral dorsal clusters. At 14-15 hours AEL numerous filopodia emerge from the primary dendritic branches of the mutant ddaE neuron (as occurs in wild type), however, the total length and the number of these immature secondary branches are decreased compared to those of wild type and the secondary branches do not elongate in a straight manner towards the posterior segment boundaries at 16-17 hours AEL.

The dendritic arbor of the vpda neuron appears normal in mutant embryos.

The dendritic arbor of the class IV ddaC neuron is deformed in mutant embryos, which appears to be due to undergrowth along the anterior-posterior axis and/or more biased elongation towards the dorsal midline.

(Sato et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

cv-c¹²⁶⁰ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Scer\GAL4^NP2225/Rho1^N19.UAS

(Sato et al., 2010)

NOT suppressed by

Statement

Reference

cv-c¹²⁶⁰ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Scer\GAL4^NP2225/Rac1^N17.UAS

(Sato et al., 2010)

cv-c¹²⁶⁰ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Scer\GAL4^NP2225/Cdc42^N17.UAS

(Sato et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

cv-c¹²⁶⁰ has larval dorsal multidendritic neuron ddaE | embryonic stage phenotype, suppressible by Scer\GAL4^NP2225/Rho1^N19.UAS

(Sato et al., 2010)

cv-c¹²⁶⁰ has dendritic tree | embryonic stage phenotype, suppressible by Scer\GAL4^NP2225/Rho1^N19.UAS

(Sato et al., 2010)

NOT suppressed by

Statement

Reference

cv-c¹²⁶⁰ has larval dorsal multidendritic neuron ddaE | embryonic stage phenotype, non-suppressible by Scer\GAL4^NP2225/Rac1^N17.UAS

(Sato et al., 2010)

cv-c¹²⁶⁰ has larval dorsal multidendritic neuron ddaE | embryonic stage phenotype, non-suppressible by Scer\GAL4^NP2225/Cdc42^N17.UAS

(Sato et al., 2010)

cv-c¹²⁶⁰ has dendritic tree | embryonic stage phenotype, non-suppressible by Scer\GAL4^NP2225/Rac1^N17.UAS

(Sato et al., 2010)

cv-c¹²⁶⁰ has dendritic tree | embryonic stage phenotype, non-suppressible by Scer\GAL4^NP2225/Cdc42^N17.UAS

(Sato et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rho1^N19.Scer\UAS under the control of Scer\GAL4^NP2225 suppresses the reduction in length of secondary dendritic branches which is seen in the ddaE neurons of cv-c¹²⁶⁰ embryos at 20-22 hours after egg laying.

Expression of either Rac1^N17.Scer\UAS or Cdc42^N17.Scer\UAS under the control of Scer\GAL4^NP2225 does not suppress the reduction in length of secondary dendritic branches which is seen in the ddaE neurons of cv-c¹²⁶⁰ embryos at 20-22 hours after egg laying.

(Sato et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

cv-c¹²⁶⁰ is rescued by Scer\GAL4^NP2225/cv-c^S.UAS

(Sato et al., 2010)

cv-c¹²⁶⁰ is rescued by Scer\GAL4^NP2225/cv-c^S.UAS.EGFP

(Sato et al., 2010)

cv-c¹²⁶⁰ is rescued by cv-c^L.UAS/Scer\GAL4^NP2225

(Sato et al., 2010)

cv-c¹²⁶⁰ is rescued by Scer\GAL4^NP2225/cv-c^L.UAS.EGFP

(Sato et al., 2010)

Partially rescued by

cv-c¹²⁶⁰ is partially rescued by cv-c^S.UAS/Scer\GAL4^pk-IG1-1

(Sato et al., 2010)

cv-c¹²⁶⁰ is partially rescued by Scer\GAL4^NP2225/cv-c^ΔS.S.UAS

(Sato et al., 2010)

Not rescued by

cv-c¹²⁶⁰ is not rescued by Scer\GAL4^NP2225/cv-c^R601L.S.UAS

(Sato et al., 2010)

cv-c¹²⁶⁰ is not rescued by Scer\GAL4^NP2225/cv-c^ΔN.S.UAS

(Sato et al., 2010)

cv-c¹²⁶⁰ is not rescued by Scer\GAL4^NP2225/cv-c^ΔC.S.UAS

(Sato et al., 2010)

Comments

Expression of any one of cv-c^S.Scer\UAS, cv-c^{S.Scer\UAS.T:Avic\GFP-EGFP}, cv-c^L.Scer\UAS or cv-c^{L.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^NP2225 rescues the dendritic defects seen in the ddaE neurons of cv-c¹²⁶⁰ embryos at 20-22 hours after egg laying (the reduction in length and number of the secondary branches is recovered to normal and the directional growth of the secondary branches along the anterior-posterior axis is also rescued).

Expression of cv-c^S.Scer\UAS under the control of Scer\GAL4^IG1-1 partially rescues the dendritic defects seen in the ddaE neurons of cv-c¹²⁶⁰ embryos at 20-22 hours after egg laying (the reduction in length of the secondary branches is not rescued but the reduction in the number of secondary branches does appear to be rescued to normal).

Expression of either cv-c^{R601L.S.Scer\UAS}, cv-c^{ΔN.S.Scer\UAS}, or cv-c^{ΔC.S.Scer\UAS} under the control of Scer\GAL4^NP2225 does not rescue the reduction in length of secondary dendritic branches which is seen in the ddaE neurons of cv-c¹²⁶⁰ embryos at 20-22 hours after egg laying.

Expression of cv-c^{ΔS.S.Scer\UAS} under the control of Scer\GAL4^NP2225 partially rescues the reduction in length of secondary dendritic branches which is seen in the ddaE neurons of cv-c¹²⁶⁰ embryos at 20-22 hours after egg laying.

(Sato et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

cv-c¹²⁶⁰

(Sato et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (1)

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