FlyBase Allele Report: Dmel\HDAC6[KO]

General Information

Symbol

Dmel\HDAC6^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0248834

Feature type

allele

Associated gene

Dmel\HDAC6

Associated Insertion(s)

Carried in Construct

Also Known As

dHDAC6^KO

Genomic Maps

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Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Du et al., 2010)

Mutagen

(Du et al., 2010)

(Du et al., 2010)

(Du et al., 2010)

(Du et al., 2010)

ends-in gene targeting

(Du et al., 2010)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

complex substitution

X:15,334,058..15,334,065 [+]

Inserted_sequence:

AGATCT

Comment:

Mutation in ATG codon and frameshift introduced by homologous recombination. AAC[ATG]GT (start codon indicated) replaced with AGATCT.

(Du et al., 2010)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Mutation in the translation start codon and a frame shift in the HDAC6 open reading frame.

(Du et al., 2010)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of obesity

CEA

(Yan et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(Du et al., 2010)

ameliorates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Xiong et al., 2013)

modeled by Hsap\MAPT^UAS.cWa

(Xiong et al., 2013)

model of obesity

is ameliorated by Hsc70-4^UAS.cEa

(Yan et al., 2017)

is ameliorated by Lsd-2^HMS00629

(Yan et al., 2017)

is ameliorated by Lsd-2^KG00149

(Yan et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

fertile

(Du et al., 2010)

neurophysiology defective

(Miskiewicz et al., 2014)

touch perception defective

(Yan et al., 2018)

touch perception defective (with Df(3L)BSC113)

(Yan et al., 2018)

touch response defective | larval stage

(Yan et al., 2018)

touch response defective | larval stage (with Df(3L)BSC113)

(Yan et al., 2018)

viable

(Du et al., 2010)

Phenotype Manifest In

cytoplasmic microtubule | third instar larval stage

(Mao et al., 2017)

embryonic/larval somatic muscle | third instar larval stage

(Mao et al., 2017)

synapse

(Miskiewicz et al., 2014)

synaptic vesicle

(Miskiewicz et al., 2014)

t-bar

(Miskiewicz et al., 2014)

Detailed Description

Statement

Reference

HDAC6^KO mutant larvae show a significantly increased response to gentle touch, as compared to controls.

(Yan et al., 2018)

HDAC6^KO mutant third instar larvae display mild but significant increase in the density of perinuclear microtubule network in muscle cells compared to controls at room temperature and their microtubules (MT) are also more resistant to cold-induced destabilization (the perinuclear MTs are significantly denser in HDAC6^KO mutant muscle cells than in wild-type after cold treatment).

(Mao et al., 2017)

HDAC6^KO mutants do not exhibit macro-morphological changes in synaptic features, with the number of T-bars, synaptic vesicles, or mitochondria per boutonic area, not differing from controls. However, using serial-section election microscopy, HDAC6^KO mutant T-bars exhibit significantly smaller 'top sizes' and fewer tethered synaptic vesicles compared to controls.

At low-frequency stimulation, the excitatory junctional current amplitude in HDAC6^KO mutants is marginally smaller.

(Miskiewicz et al., 2014)

Homozygotes show no obvious morphological abnormalities under normal culture conditions.

Mutants do not show any notable difference to wild-type animals in response to oxidative or heat stress.

Mutant adults do not show defects in climbing ability (assayed up to 20 days after eclosion).

(Du et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

HDAC6^KO has touch response defective | larval stage phenotype, non-enhanceable by αTub84B^K40A/αTub84B^K40A

(Yan et al., 2018)

HDAC6^KO has touch perception defective phenotype, non-enhanceable by αTub84B^K40A/αTub84B^K40A

(Yan et al., 2018)

Enhancer of

Statement

Reference

HDAC6^KO is an enhancer of neuroanatomy defective | conditional | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF/Scer\GAL4^ple.PF

(Du et al., 2010)

HDAC6^KO is an enhancer of locomotor behavior defective | conditional | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF/Scer\GAL4^ple.PF

(Du et al., 2010)

HDAC6^KO is an enhancer of neuroanatomy defective | conditional | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Du et al., 2010)

Suppressor of

Statement

Reference

HDAC6^KO/HDAC6[+] is a suppressor of locomotor behavior defective | adult stage phenotype of Hsap\TARDBP^{A315T.UAS.cVa}, Scer\GAL4^elav.PU

(Miskiewicz et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

HDAC6^KO is an enhancer of dopaminergic neuron | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF/Scer\GAL4^ple.PF

(Du et al., 2010)

HDAC6^KO is an enhancer of ommatidium | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Du et al., 2010)

HDAC6^KO is an enhancer of eye photoreceptor cell | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Du et al., 2010)

Suppressor of

Statement

Reference

HDAC6^KO/HDAC6[+] is a suppressor of t-bar phenotype of Hsap\TARDBP^{A315T.UAS.cVa}, Scer\GAL4^elav.PU

(Miskiewicz et al., 2014)

HDAC6^KO is a suppressor of microtubule phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^C57

(Xiong et al., 2013)

Df(1)ED7294/HDAC6^KO is a suppressor of microtubule phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^C57

(Xiong et al., 2013)

HDAC6^KO is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PU

(Xiong et al., 2013)

HDAC6^KO is a suppressor of NMJ bouton | larval stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PU

(Xiong et al., 2013)

HDAC6^KO is a suppressor of microtubule phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^C57

(Xiong et al., 2013)

Df(1)ED7294/HDAC6^KO is a suppressor of microtubule phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^C57

(Xiong et al., 2013)

HDAC6^KO is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^elav.PU

(Xiong et al., 2013)

HDAC6^KO is a suppressor of NMJ bouton | larval stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^elav.PU

(Xiong et al., 2013)

NOT Suppressor of

Statement

Reference

HDAC6^KO is a non-suppressor of microtubule phenotype of Scer\GAL4^C57, spas^UAS.cTa

(Mao et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The decrease in perinuclear microtubule density which is seen in larval muscle cells expressing spas^Scer\UAS.cTa under the control of Scer\GAL4^C57 is not altered by the presence of HDAC6^KO.

The decrease in perinuclear microtubule density which is seen in larval muscle cells expressing Kat60^Scer\UAS.cMa under the control of Scer\GAL4^C57 is not altered by the presence of HDAC6^KO. The microtubule fibres are significantly shorter in the double mutant cells.

(Mao et al., 2014)

Xenogenetic Interactions

Statement

Reference

A HDAC6^KO heterozygous background suppresses the larger synaptic T-bar size found upon expression of Hsap\TARDBP^{A315T.cMa.Scer\UAS} under the control of Scer\GAL4^elav.PU.

A HDAC6^KO heterozygous background suppresses the larger synaptic T-bar size found upon expression of Hsap\TARDBP^{A382T.cMa.Scer\UAS} under the control of Scer\GAL4^elav.PU.

While heterozygosity for HDAC6^KO alone has no effect on activity or negative geotaxis, these conditions both significant suppress the activity and geotaxis defects in flies expressing Hsap\TARDBP^{A315T.cMa.Scer\UAS} under the control of Scer\GAL4^elav.PU.

(Miskiewicz et al., 2014)

HDAC6^KO and HDAC6^KO/Df(1)ED7294 each rescue the defects in perinuclear microtubule density seen in the muscles of animals expressing either Hsap\MAPT^Scer\UAS.cWa or Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^C57.

HDAC6^KO completely suppresses the increase in the number of satellite boutons at the neuromuscular junction (NMJ) and the decrease in average NMJ length caused by expression of either Hsap\MAPT^Scer\UAS.cWa or Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^elav.PU.

(Xiong et al., 2013)

HDAC6^KO enhances the loss of dopaminergic (DA) neurons caused by expression of Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF; the neuron degeneration phenotype is apparent at 10 days after eclosion in the double mutant flies, and 20 days after eclosion it is more severe than that seen in the Hsap\SNCA^Scer\UAS.cFa, Scer\GAL4^ple.PF single mutants.

HDAC6^KO enhances the retinal degeneration phenotype seen in adults expressing Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PU.

HDAC6^KO enhances the climbing ability defect caused by expression of Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF; defects are seen 10 days after eclosion in the double mutant flies (defects are not seen until 20 days after eclosion in Hsap\SNCA^Scer\UAS.cFa, Scer\GAL4^ple.PF single mutants) and the defects at 20 days after eclosion are more severe in the double mutants.

HDAC6^KO decreases the number of Lewy body-like Hsap\SNCA positive inclusions seen in the brains of adults expressing Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF such that no inclusions are seen in 10 day old flies and the number of inclusions seen in 20 days old flies is reduced.

(Du et al., 2010)

Complementation and Rescue Data

Rescued by

HDAC6^KO is rescued by HDAC6^UAS.cDa/Scer\GAL4^C57

(Xiong et al., 2013)

HDAC6^KO is rescued by Scer\GAL4^C57/HDAC6^H237A.UAS

(Xiong et al., 2013)

Partially rescued by

HDAC6^KO is partially rescued by HDAC6^H664A.UAS/Scer\GAL4^C57

(Xiong et al., 2013)

Not rescued by

HDAC6^KO is not rescued by HDAC6^{H237A.H664A.UAS}/Scer\GAL4^C57

(Xiong et al., 2013)

Comments

Expression of either HDAC6^Scer\UAS.cDa or HDAC6^{H237A.Scer\UAS} under the control of Scer\GAL4^C57 completely suppresses the ability of HDAC6^KO to rescue the perinuclear microtubule density defects seen in the muscles of animals expressing either Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^C57.

Expression of HDAC6^{H664A.Scer\UAS} under the control of Scer\GAL4^C57 partially suppresses the ability of HDAC6^KO to rescue the perinuclear microtubule density defects seen in the muscles of animals expressing either Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^C57.

Expression of HDAC6^{H237A.H664A.Scer\UAS} under the control of Scer\GAL4^C57 does not affect the ability of HDAC6^KO to rescue the perinuclear microtubule density defects seen in the muscles of animals expressing either Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^C57.

(Xiong et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51182

y¹ w^* HDAC6^KO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

HDAC6(KO)

(Perry et al., 2017)

HDAC6^KO

(Yan et al., 2018, Mao et al., 2017, Mao et al., 2014, Xiong et al., 2013)

HDAC6^ko

(Miskiewicz et al., 2014)

HDAC6KO

(Vargas et al., 2020)

Hdac6Δ

(Dobbelaere et al., 2020)

dHDAC6^KO

(Yan et al., 2017, Du et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (10)

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