FlyBase Allele Report: Scer\GAL4[GMR52A06]

General Information

Symbol

Scer\GAL4^GMR52A06

Species

S. cerevisiae

Name

FlyBase ID

FBal0253256

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{GMR52A06-GAL4}

Also Known As

R52A06-GAL4

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Pfeiffer et al., 2011.2.9)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GMR52A06-GAL4}

Cytology

Nature of the lesion

Statement

Reference

A 3876 base pair fragment from the flanking non-coding or intronic region of cdi is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Allele components

Product class / Tool use(s)

Encoded product / tool

binary expression system - driver

Associated Sequence Features

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult gnathal ganglion | faint | restricted

(Jenett and Svirskas, 2012.9.24)

adult gnathal ganglion | intense | restricted

(Jenett and Svirskas, 2012.9.24)

adult antennal lobe | faint | restricted

(Jenett and Svirskas, 2012.9.24)

antennal mechanosensory and motor center | intense | restricted

(Jenett and Svirskas, 2012.9.24)

saddle | intense | restricted

(Jenett and Svirskas, 2012.9.24)

mechanosensory neuron of interommatidial bristle

(Hampel et al., 2017)

sensory neuron of head bristle

(Hampel et al., 2017)

sensory neuron of antennal segment 1 microchaeta | subset

(Hampel et al., 2017)

sensory neuron of antennal segment 2 microchaeta | subset

(Hampel et al., 2017)

Johnston organ neuron activated by antennal displacement

(Hampel et al., 2017)

sensory neuron of maxillary palp bristle

(Hampel et al., 2017)

sensory neuron of proboscis

(Hampel et al., 2017)

Wheeler's organ

(Hampel et al., 2017)

mechanosensory chaeta of adult abdomen | ventral

(Hampel et al., 2017)

mechanosensory chaeta of adult abdomen | dorsal

(Hampel et al., 2017)

mechanosensory neuron of adult abdomen | ventral

(Hampel et al., 2017)

mechanosensory neuron of adult abdomen | dorsal

(Hampel et al., 2017)

multidendritic neuron | subset of adult abdomen

(Hampel et al., 2017)

sensory neuron of genitalia

(Hampel et al., 2017)

sensory neuron | subset of sensillum campaniformium of ventral radius

(Hampel et al., 2017)

sensory neuron of sensillum campaniformium of distal group

(Hampel et al., 2017)

sensory neuron of wing margin bristle

(Hampel et al., 2017)

mechanosensory neuron of notal microchaeta

(Hampel et al., 2017)

sensory neuron of leg taste bristle

(Hampel et al., 2017)

sensory neuron of prothoracic coxal bristle on hairy island

(Hampel et al., 2017)

scolopidial neuron of chordotonal organ of leg

(Hampel et al., 2017)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal grooming behavior | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Hampel et al., 2017)

abnormal grooming behavior | conditional, with Crei\ChR2^UAS.cSa

(Hampel et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Site-directed optogenetic activation (using Crei\ChR2^Scer\UAS.cSa with blue light) of Scer\GAL4^GMR52A06 neurons induces significant grooming behavior compared to control flies; light directed to the posterior dorsal body surface elicits wing grooming whereas illumination of the anterior dorsal surface elicits notum grooming.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with red light) of Scer\GAL4^GMR52A06 neurons across the body induces significant grooming behavior; flies groom their heads at the onset of a five-second red light stimulus and then transition to groom their posterior bodies during the period after the light is turned off. Even when these flies are engaged in posterior grooming, delivery of the next red light stimulus causes flies to switch to head grooming. In decapitated flies, red-light activation of Scer\GAL4^GMR52A06 neurons directly elicits immediate posterior grooming behavior.

(Hampel et al., 2017)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

38810

w¹¹¹⁸; P{GMR52A06-GAL4}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^GMR52A06

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections