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General Information
Symbol
Dmel\Vps16B1
Species
D. melanogaster
Name
FlyBase ID
FBal0258124
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
fob1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The entire Vps16B open reading frame has been replaced by a w+mW.hs marker.

Insertion components
TI{TI}Vps16B1
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Vps16B1 homozygous adults show significant decrease in lifespan upon infection with heat-killed E.coli bacteria, as compared to infected controls.

Vps16B1 flies exhibit a profound defect in phagosome maturation, and as a consequence are sensitive to infections with normally non-pathogenic microbes. Vps16B1mutants are strongly susceptible to infections with Escherichia coli (that are not normally pathogenic to flies).

Pathogenic Enterococcus faecalis microbes kill Vps16B1 flies only slightly faster than wild-type.

Vps16B1 hemocytes do not exhibit a defect in initial phagocytic uptake of bacteria. After 45 minutes however, Vps16B1 mutant hemocytes are full of bacteria and do not seem able to digest them. The bacterial burden remains elevated, as opposed to the efficient clearance of the majority of bacteria in wild-type flies after just 1 day.

Vps16B1 phagosomes fail to acidify.

Eyes of two-day old Vps16B1 mutants exhibit normal ommatidial organization.

Starvation-induced autophagy, which requires fusion with lysosomes, is normal in Vps16B1 larvae.

Vps16B1 flies exhibit normal lysosomal function.

Vps16B1 flies do not show any morphological defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The survival of Vps16B1 flies is significantly improved through expression of Vps16BScer\UAS.T:Hsap\MYC in hemocytes under the control of Scer\GAL4srp.Hemo or ubiquitously under the control of Scer\GAL4da.G32.

Expression of Vps16B+t significantly improves survival of Vps16B1 flies.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)