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General Information
Symbol
Scer\GAL4alrm.PD
Species
S. cerevisiae
Name
astrocytic leucine-rich repeat molecule promoter construct of Doherty
FlyBase ID
FBal0260884
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
alrm-Gal4, alrmGal4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 4973bp region of the alrm promoter is fused upstream of sequence encoding a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in reticular neuropil associated glial cells.

The larval neuropil glial cells are Gs2-positive and comprise Scer\GAL4alrm.PD-positive, pros-positive and Scer\GAL4NP6520-positive, pros-negative subsets.

Scer\GAL4alrm.PD drives expression in a subset of distal medulla astrocyte-like glial cells with processes to medulla layer M7.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Thermogenetic activation (using TrpA1UAS.(B).cKa at 28[o]C) of Scer\GAL4alrm.PD cells in adults significantly reduces the amplitude and slope of excitatory postsynaptic potentials in antennal lobe projection neurons in response to activation of the antennal nerve, compared to controls, with no effect on onset latency.

Expression of hid in Scer\GAL4alrm.PD expressing neurons (limited to late metamorphosis using Scer\GAL80ts.αTub84B and shifting pupae to 25[o]) induces astrocyte ablation. A ~50% reduction in astrocyte numbers is seen in the antennal lobe, mushroom body, subesophageal ganglion (SOG) and abdominal segments of the thoracic ganglion (TGab), but the remaining astrocytes are able to infiltrate most of the neuropil with membrane processes. No lethality is observed and the adult flies do not display any obvious behavioral defects. Shifting the pupae to 30[o]C leads to more dramatic astrocyte reduction, with the remaining astrocyte processes appearing sparsely distributed and unable to cover the neuropil space. 41% of pupae are unable to eclose, 32% die during eclosion and only 27% survive as adults. These surviving adults are highly uncoordinated, cannot walk or fly, and die within a few days.

Gross synaptic morphology is unaffected by severe astrocyte ablation. Postsynaptic density length is unchanged and the percentage of synapses with T-bars is unaltered. Synaptic density is reduced in the antennal lobe, mushroom body and superior posterior slope. The astrocyte-ablated tissue is also marked by an increased frequency of ruptured mitochondria. At 84h after pupal formation, synapse numbers are significantly reduced, but several immature synaptic structures are seen. Gross morphology of the adult brain appears unaffected by severe ablated depletion: antennal lobe glomerular organisation appears normal, as are the neuronal projections of second-order antennal lobe projection neurons and pigment dispersing factor (pdf) neurons. The number of projection neurons is comparable in control and astrocyte ablated animals, as are pdf neurons. The membranes of cortex and ensheathing glia in the adult brain exhibit normal morphology.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4alrm.PD
Name Synonyms
astrocytic leucine-rich repeat molecule promoter construct of Doherty
Secondary FlyBase IDs
    References (65)