The expression of NosdsRNA.UAS.IR-X under the control of Scer\GAL4phm.PO leads to an enlarged ring gland in third instar larvae, which is red-brownish in brightfield and red auto-fluorescent to UV.
Expression of NosdsRNA.Scer\UAS.IR-X (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4elav.PU does not disrupt the growth inhibition undamaged (anterior) tissues - eye discs - in third instar larvae (104 hr after egg laying, AEL) following targeted irradiation (limited to posterior tissues and administered in late second instar, at 80 hr AEL) which is observed in wild-type larvae neither does it affect the developmental delay the regenerative growth of damaged tissues normally produces.
Expression of NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4phm.PO has no effect on prothoracic gland viability.
Over 80% of larvae expressing NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4phm.PO fail to pupariate on time, continuing to feed until reaching a maximum size and weight 5 to 7 days after the normal time of metamorphosis. Some larvae continue to feed and wander for as long as 30-40 days. The relatively few larvae that initiate metamorphosis after day 5 exhibit obvious defects and fail to eclose. The majority of Scer\GAL4phm.PO> NosdsRNA.Scer\UAS.IR-X larvae initiate metamorphosis when their food is supplemented with ecdysone.
Expression of NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4phm.PO results in an average 1.5 times increase in larval length.
The prothoracic glands in embryos expressing NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4phm.PO grow as much as six times their normal size, while the adjacent brain hemispheres show no relative change. This growth is due to an increase in prothoracic gland cell size, not number.
Despite larvae expressing NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4phm.PO growing considerably larger than their wild-type counterparts, their central nervous systems and imaginal discs remain the same size, with the same approximate numbers of cells. However, fat bodies, which extend throughout most of the larva, are nearly two times their normal size. Thus, much of the overall size change of NosdsRNA.Scer\UAS.IR-X-expressing larva appears to be attributable to the fat body.
The number of cytoplasmic lipid vesicles is sixfold higher in larvae expressing NosdsRNA.Scer\UAS.IR-X in the thoracic glands under the control of Scer\GAL4phm.PO. In addition, vesicle diameters are more than twofold higher. Remarkably, the levels of larval triacylglycerol, which is representative of overall lipid content, are ~14-fold higher in NosdsRNA.Scer\UAS.IR-X 10 day old larvae.