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General Information
Symbol
Dmel\NosdsRNA.UAS.IR-X
Species
D. melanogaster
Name
FlyBase ID
FBal0263808
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a Nos RNAi line.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of NosdsRNA.UAS.IR-X under the control of Scer\GAL4phm.PO leads to an enlarged ring gland in third instar larvae, which is red-brownish in brightfield and red auto-fluorescent to UV.

Expression of NosdsRNA.Scer\UAS.IR-X (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4elav.PU does not disrupt the growth inhibition undamaged (anterior) tissues - eye discs - in third instar larvae (104 hr after egg laying, AEL) following targeted irradiation (limited to posterior tissues and administered in late second instar, at 80 hr AEL) which is observed in wild-type larvae neither does it affect the developmental delay the regenerative growth of damaged tissues normally produces.

Expression of NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4elav-C155 or Scer\GAL4ey-OK107, but not Scer\GAL4repo.PU leads to a significant increase in axonal regrowth of mushroom body gamma neurons in P-stage brains both in vivo or cultured ex vivo, as compared to wild type.

Expression of NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4phm.PO has no effect on prothoracic gland viability.

Over 80% of larvae expressing NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4phm.PO fail to pupariate on time, continuing to feed until reaching a maximum size and weight 5 to 7 days after the normal time of metamorphosis. Some larvae continue to feed and wander for as long as 30-40 days. The relatively few larvae that initiate metamorphosis after day 5 exhibit obvious defects and fail to eclose. The majority of Scer\GAL4phm.PO> NosdsRNA.Scer\UAS.IR-X larvae initiate metamorphosis when their food is supplemented with ecdysone.

Expression of NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4phm.PO results in an average 1.5 times increase in larval length.

The prothoracic glands in embryos expressing NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4phm.PO grow as much as six times their normal size, while the adjacent brain hemispheres show no relative change. This growth is due to an increase in prothoracic gland cell size, not number.

Despite larvae expressing NosdsRNA.Scer\UAS.IR-X under the control of Scer\GAL4phm.PO growing considerably larger than their wild-type counterparts, their central nervous systems and imaginal discs remain the same size, with the same approximate numbers of cells. However, fat bodies, which extend throughout most of the larva, are nearly two times their normal size. Thus, much of the overall size change of NosdsRNA.Scer\UAS.IR-X-expressing larva appears to be attributable to the fat body.

The number of cytoplasmic lipid vesicles is sixfold higher in larvae expressing NosdsRNA.Scer\UAS.IR-X in the thoracic glands under the control of Scer\GAL4phm.PO. In addition, vesicle diameters are more than twofold higher. Remarkably, the levels of larval triacylglycerol, which is representative of overall lipid content, are ~14-fold higher in NosdsRNA.Scer\UAS.IR-X 10 day old larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
NOT suppressed by
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Coexpression of Hr46NIG.12208R with NosdsRNA.Scer\UAS.IR-X in prothoracic glands (with both transgenes under the control of Scer\GAL4phm.PO) results in prolonged larval growth, as in the single mutants.

Coexpression of Hr46Scer\UAS.cTa with NosdsRNA.Scer\UAS.IR-X in prothoracic glands (with both transgenes under the control of Scer\GAL4phm.PO) suppresses the larval overgrowth phenotype and results in small larvae.

Coexpression of Eip75BA.Scer\UAS.cCa with NosdsRNA.Scer\UAS.IR-X in prothoracic glands (with both transgenes under the control of Scer\GAL4phm.PO) results in prolonged larval growth, as in the single mutants.

Coexpression of Eip75BGD1434 with NosdsRNA.Scer\UAS.IR-X in prothoracic glands (with both transgenes under the control of Scer\GAL4phm.PO) suppresses the larval overgrowth phenotype and results in small larvae.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
NosdsRNA.Scer\UAS.IR-X
NosdsRNA.UAS.IR-X
Name Synonyms
Secondary FlyBase IDs
    References (5)