The morphology of the mushroom bodies and central complex appears grossly normal in mutant adult brains and the volume of the mushroom body calyx and of the central complex is not significantly different from that of wild type.

Homozygous adults have a normal avoidance of electric shock and of the odours octanol and benzaldehyde at the concentrations using for olfactory learning assays. The mutant adults show normal performance in negative geotaxis and fast phototaxis assays.

Mutant adults have a decreased performance index immediately after training in an olfactory learning assay. The performance indices are also reduced at 30 minutes and 6 hours after training in the mutant flies, but the difference between mutant and wild type is consistent over these short-term (30 minutes) and middle-term (6 hour) phases of memory, suggesting normal memory decay in the mutants.

The external genitalia of mutant males and females are indistinguishable from wild type. The morphology of the sex combs is intact in the mutant males, although the number of bristles in the sex comb is slighter lower than that of controls.

Mutant males perform all of the necessary courting rituals, but spend 26% less time courting than do wild-type males. Similar to wild-type males, mutant males mount the female and curl their abdomen to begin copulation. However, after coupling, the mutant male continuously struggles to maintain his orientation and can be seen in a variety of different positions relative to the female. The mating pairs move about the observation chamber, with the female dragging the male behind. These copulation defects are seen in pairs where mutant males are mated to wild-type females, but are not seen in pairs where wild-type males are mated to mutant females.

The copulation defects seen in homozygous males are phenocopied in prt¹/Df(3R)mbc-30 and prt¹/Df(3R)Exel6195 males.

Despite the copulation defects seen in mutant males, homozygous mating pairs produce approximately half the number of offspring of wild-type mated pairs. The duration of copulation is reduced in homozygous mating pairs compared to wild-type mated pairs.