FlyBase Allele Report: Dmel\Rh5[2]

General Information

Symbol

Dmel\Rh5²

Species

D. melanogaster

Name

FlyBase ID

FBal0265961

Feature type

allele

Associated gene

Dmel\Rh5

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Yamaguchi et al., 2008)

Progenitor genotype

P{GT1}BG01539

(Yamaguchi et al., 2008)

Cytology

Description

FLPase-mediated excision of P{GT1}BG01539, located 1.2kb upstream of Rh5, and P{EP}EP574, found downstream of the Rh5 ORF.

(Yamaguchi et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of sensorineural hearing loss

CEA

(Senthilan et al., 2012)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates sensorineural hearing loss

modeled by Rh6¹

(Senthilan et al., 2012)

model of sensorineural hearing loss

is exacerbated by Rh6¹

(Senthilan et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception | adult stage

(Senthilan et al., 2012)

abnormal neurophysiology | adult stage

(Senthilan et al., 2012)

abnormal phototaxis

(Keene et al., 2011)

abnormal thermotaxis | third instar larval stage

(Sokabe et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Rh5² homozygous mutant mid and late third instar (96 and 120 hr AEL, respectively) larvae show impaired temperature selection and unlike controls display no strong preference for cooler temperatures (18[o]C) in a temperature gradient. This effect does not appear to be due to developmental delay as the mutants develop with similar timing as controls, they also do not show any gross defects in the morphology of TrpA1 neurons, 18[o]C versus 28[o]C thermotaxis or crawling speed.

(Sokabe et al., 2016)

Mutant larvae show normal levels of avoidance in response to green light.

(Liu et al., 2014)

Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. The displacement response of the antenna over a range of sound particle velocities is linearised, indicating loss of mechanical amplification.

(Senthilan et al., 2012)

Light avoidance is completely lost in Rh5² mutants.

(Keene et al., 2011)

Rh5² mutant class IV dendritic arborization neurons do not exhibit any defects in light response.

(Xiang et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rh5², cry^b has abnormal locomotor rhythm phenotype, enhanceable by Rh6¹

(Szular et al., 2012)

Rh5², cry^b, norpA^P41 has abnormal locomotor rhythm phenotype, enhanceable by Rh6¹

(Szular et al., 2012)

NOT Enhanced by

Statement

Reference

Rh5² has abnormal thermotaxis | third instar larval stage phenotype, non-enhanceable by Rh6¹

(Sokabe et al., 2016)

Rh5² has abnormal phototaxis phenotype, non-enhanceable by Rh6¹

(Keene et al., 2011)

NOT suppressed by

Statement

Reference

Rh5² has abnormal phototaxis phenotype, non-suppressible by Rh6¹

(Keene et al., 2011)

Enhancer of

Statement

Reference

Rh5² is an enhancer of abnormal locomotor rhythm phenotype of Rh6¹, cry^b, norpA^P41

(Szular et al., 2012)

Rh5² is an enhancer of abnormal locomotor rhythm phenotype of Rh6¹, cry^b

(Szular et al., 2012)

Rh5²/Rh6¹ is an enhancer of abnormal locomotor rhythm phenotype of cry^b

(Szular et al., 2012)

Rh5² is an enhancer of abnormal locomotor rhythm phenotype of cry^b, norpA^P41

(Szular et al., 2012)

Rh5²/Rh6¹ is an enhancer of abnormal locomotor rhythm phenotype of cry^b, norpA^P41

(Szular et al., 2012)

NOT Enhancer of

Statement

Reference

Rh5² is a non-enhancer of abnormal thermotaxis | third instar larval stage phenotype of Rh6¹

(Sokabe et al., 2016)

Rh5² is a non-enhancer of abnormal locomotor rhythm phenotype of cry^b

(Szular et al., 2012)

Suppressor of

Statement

Reference

Rh5², Rh5², Rh6¹, Rh6¹ is a suppressor of abnormal circadian rhythm | adult stage phenotype of cry⁰¹

(Ni et al., 2017)

Other

Statement

Reference

Rh5[+]/Rh5², tor^e00150 has abnormal phototaxis | dominant | third instar larval stage phenotype

(Yamanaka et al., 2013)

Rh5², Rh6¹ has abnormal neurophysiology | adult stage phenotype

(Senthilan et al., 2012)

Rh5², Rh6¹ has abnormal auditory perception | adult stage phenotype

(Senthilan et al., 2012)

Rh5², Rh6¹ has abnormal neuroanatomy | third instar larval stage phenotype

(Yuan et al., 2011)

Rh5², Rh6¹, sev¹ has abnormal optomotor response phenotype

(Yamaguchi et al., 2008)

Phenotype Manifest In

Other

Statement

Reference

Rh5², Rh6¹ has dendrite | third instar larval stage phenotype

(Yuan et al., 2011)

Rh5², Rh6¹ has LNv neuron | third instar larval stage phenotype

(Yuan et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Rh5², Rh6¹ double homozygosity rescues the circadian rhythmicity defects of cry⁰¹ homozygotes.

(Ni et al., 2017)

The impaired thermotactic behavior characteristic for Rh5² and Rh6¹ single mutant late third instar larvae (120 hr AEL) does not deteriorate further in the Rh5²;Rh6¹ double mutants.

(Sokabe et al., 2016)

The sound threshold required to evoke a compound action potential in the antennal nerve in response in Rh5² Rh6¹ double mutants is almost twice as loud as required in single mutants. Ultrastructure of the Johnston's organ sensillum appears normal in the double mutant flies.

(Senthilan et al., 2012)

Following a 6 hour delay in their light-dark cycle, cry^b Rh5² flies show a similar delay in resynchronizing their evening activity peak as cry^b flies. Triple mutant cry^b Rh5² Rh6¹ flies resynchronize over a longer period than either the cry^b single or double mutants.

Following a 6 hour delay in their light-dark cycle, cry^b norpA³⁹ flies show a longer delay in resynchronizing their evening activity peak (5-7 days) compared to cry^b flies. The addition of Rh5² to this background extends the time required for resynchronization to beyond 7 days. The addition of both Rh5² and Rh6¹ to the cry^b norpA³⁹ background results in minimal (if any) tendency to resynchronize circadian behaviour.

Following a 6 hour advancement in their light-dark cycle, cry^b norpA³⁹ flies show a longer delay in resynchronizing their circadian behaviour (several days) compared to either single mutant. Introducing Rh5² to this background interferes more strongly with resynchronization, while the quadruple cry^b norpA³⁹ Rh5² Rh6¹ mutant shows the strongest phenotype.

(Szular et al., 2012)

Rh5²;Rh6¹ double mutants are completely blind, with light avoidance being completely lost at the larval stage.

Rh5²;Rh6¹ double mutants eclose at a similar rate as in wild-type.

Despite Rh5²;Rh6¹ double mutants having a decreased ability to degrade tim in response to light, they are still capable of proper circadian behavior.

(Keene et al., 2011)

Wild-type larvae display differences in their LN(v) dendrite length when exposed to constant darkness versus constant light. In contrast, such light induced changes are absent in Rh5², Rh6¹ double mutants.

(Yuan et al., 2011)

sev¹, Rh5², Rh6¹ triple mutant flies do not have R7 cells and do not express function R8 opsins, therefore colour vision should be completely abolished. However, the blue/green intensity-response curve for these flies appears indistinguishable from wild-type.

(Yamaguchi et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rh5²/Rh5^G is rescued by Rh5^UAS.cSa/Scer\GAL4^Rh5-G

(Sokabe et al., 2016)

Comments

Expression of Rh5^Scer\UAS.cSa in under the control of Scer\GAL4^Rh5-G in the Rh5²/Rh5^G transheterozygote larvae (using the intrinsic Gal4 driver in Rh5^G) rescues the impaired thermotactic behavior characteristic for Rh5² mutants.

(Sokabe et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Rh5²

(Xiao et al., 2023, Sorkaç et al., 2022, Alejevski et al., 2019, Liu et al., 2014, Senthilan et al., 2012, Szular et al., 2012, Keene et al., 2011, Xiang et al., 2010, Yamaguchi et al., 2008)

Rh5[2]

(Yamanaka et al., 2013)

rh5⁰²

(Ni et al., 2017)

rh5²

(Yuan et al., 2021, Leung et al., 2020, Dombrovski et al., 2019, Schlichting et al., 2019, Sokabe et al., 2016, Vasiliauskas et al., 2011, Yuan et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (18)

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