Open Close
General Information
Symbol
Dmel\lin-5293
Species
D. melanogaster
Name
FlyBase ID
FBal0277116
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

1302 bp deletion resulting from the imprecise excision of P{EP}EP404.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{EP} element in P{EP}EP404 has resulted in a 1302bp deletion that removes the entire lin-52 gene.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Eyes composed entirely of lin-5293 mutant clone cells (generated using the ey-FLP method) are rough and include small rhabdomeres and irregular ommatidia. When lin-5293 mutant eye clones are generated alongside wild type control tissue mutant the mutant ommatidia are disorganised and contain rhabdomeres that are distended and/or absent compared to the adjacent control cells. No defects are observed in the control cells, indicating that the phenotype is cell-autonomous.

Less than 1% of embryos derived from females carrying homozygous lin-5293 germline clones crossed to wild type males are able to hatch. in 86% of embryos mitotic arrest occurs when the embryo contains between two and eight nuclei. In many of these embryos the mitotic spindles and metaphase plate have failed to form correctly, with condensed chromosomes often distributed diffusely between the spindle poles. One or both centrosomes are often missing and other defects observed include a broad spindle with no centrosomes, a branched spindle with no centrosomes or an elongated spindle with no centrosomes.

Generation of lin-5293 mutant clones in the germline or ovarian somatic cells has no effect on the morphology of these cells, nor on the progression of oogenesis.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference

lin-5293 has embryo | germline clone | maternal effect phenotype, non-suppressible by mip40[+]/mip40EY16520

lin-5293 has eye | somatic clone phenotype, non-suppressible by MybMH107

Additional Comments
Genetic Interactions
Statement
Reference

mip40EY16520 rescues the lethality seen in homozygous and hemizygous lin-5293 larvae. Both male and female adult flies are seen at a similar frequency to controls. One copy of mip40EY16520 is not sufficient to rescue the lethality.

Homozygous mip1301-36 suppresses the eye phenotypes seen in lin-5293 mutant eye clones. The mutant tissue appears completely normal.

Homozygous MybMH107 does not suppress the eye phenotypes seen in lin-5293 mutant eye clones.

Homozygous mip1301-36 suppresses the eye phenotypes seen in MybMH107, lin-5293 double mutant clones. The resulting triple mutant eyes appear completely normal.

Approximately 29% of embryos derived from females carrying homozygous lin-5293 mip1301-36 double mutant germline clones crossed to wild type males are able to hatch, a partial suppression of the phenotype seen when females are maternally mutant for lin-5293 alone.

Approximately 2% of embryos derived from females carrying homozygous lin-5293 MybMH107 double mutant germline clones crossed to wild type males are able to hatch, a similar rate to when females are maternally mutant for lin-5293 alone.

Embryos derived from females that carry homozygous lin-5293 mutant germline clones and are heterozygous for mip40EY16520 crossed to wild type males are unable to hatch, similar to when females are maternally mutant for lin-5293 alone.

Approximately 21% of embryos derived from females that carry homozygous lin-5293 mutant germline clones and are heterozygous for mip12067 (Df(2R)mip12067 mutants in which EfTuM[+] function has been restored using EfTuM+tBa) crossed to wild type males are able to hatch, a partial suppression of the phenotype seen when females are maternally mutant for lin-5293 alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of lin-52+t1.6 rescues the eye phenotypes seen in eye composed entirely of lin-5293 mutant somatic clone cells.

Expression of lin-52+t1.6 rescues the eye phenotypes seen in eye composed entirely of lin-5293 mutant somatic clone cells.

Expression of lin-52+t1.6 rescues the embryonic lethality seen in embryos derived from females carrying homozygous lin-5293 germline clones crossed to wild type males.

Expression of lin-52T:Avic\GFP rescues the lethality seen in lin-5293 mutant larvae.

Expression of lin-52Ubi-p63E.T:Zzzz\FLAG rescues the lethality seen in lin-5293 mutant larvae.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)