FlyBase Allele Report: Dmel\Rab21[GD9402]

General Information

Symbol

Dmel\Rab21^GD9402

Species

D. melanogaster

Name

FlyBase ID

FBal0277642

Feature type

allele

Associated gene

Dmel\Rab21

Associated Insertion(s)

Carried in Construct

P{GD9402}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Jean et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Jean et al., 2012)

Mutagen

in vitro construct

(Jean et al., 2012)

Progenitor genotype

Carried in construct

P{GD9402}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Jean et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9402

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult enteroendocrine cell | increased number, with Scer\GAL4^NP0001

(Nassari et al., 2022)

adult gut, with Scer\GAL4^NP0001

(Nassari et al., 2022)

autolysosome | calorie restriction conditional, with Scer\GAL4^Cg.PA

(Jean et al., 2015)

autophagosome | increased number, with Scer\GAL4^NP0001

(Nassari et al., 2022)

cell junction | adult stage, with Scer\GAL4^NP0001

(Nassari et al., 2022)

embryonic/larval plasmatocyte, with Scer\GAL4^Cg.PA

(Jean et al., 2012)

fat body, with Scer\GAL4^Cg.PA

(Jean et al., 2015)

fat body, with Scer\GAL4^NP0001

(Nassari et al., 2022)

gut epithelium | adult stage, with Scer\GAL4^NP0001

(Nassari et al., 2022)

intestinal stem cell | adult stage | increased number, with Scer\GAL4^NP0001

(Nassari et al., 2022)

lipid droplet | adult stage, with Scer\GAL4^NP0001

(Nassari et al., 2022)

Detailed Description

Statement

Reference

Autolysosomes are normally absent in wild-type larvae under well-fed conditions but are rapidly induced with autophagy in response to starvation. Expression of Scer\GAL4^Cg.PA>Rab21^GD9042 blocks the appearance of autolysosomes in starved fat bodies.

(Jean et al., 2015)

Primary macrophages isolated from animals expressing Rab21^GD9042 under the control of Scer\GAL4^Cg.PA lack F-actin rich protrusions.

(Jean et al., 2012)

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rab21^GD9402, Scer\GAL4^NP0001 has increased occurrence of cell division | adult stage phenotype, suppressible by upd3^HMS00646, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has increased occurrence of cell division | adult stage phenotype, suppressible by yki^HMS00041, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has increased cell death | adult stage phenotype, suppressible by yki^HMS00041, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has increased occurrence of cell division | adult stage phenotype, suppressible by Egfr^DN.UAS.cBa, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has increased occurrence of cell division | adult stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^NP0001

(Nassari et al., 2022)

NOT suppressed by

Statement

Reference

Rab21^GD9402, Scer\GAL4^NP0001 has increased occurrence of cell division | adult stage phenotype, non-suppressible by Rab11^{Q70L.UASp.YFP}, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has increased occurrence of cell division | adult stage phenotype, non-suppressible by apolpp^HM05157, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has increased occurrence of cell division | adult stage phenotype, non-suppressible by Tret1^HMS02573, Scer\GAL4^NP0001

(Nassari et al., 2022)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rab21^GD9402, Scer\GAL4^Cg.PA has embryonic/larval plasmatocyte phenotype, enhanceable by Sbf^GD12081, Scer\GAL4^Cg.PA

(Jean et al., 2012)

Suppressed by

Statement

Reference

Rab21^GD9402, Scer\GAL4^NP0001 has adult gut phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has adult gut phenotype, suppressible by upd3^HMS00646, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has adult gut phenotype, suppressible by yki^HMS00041, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has adult gut phenotype, suppressible by Egfr^DN.UAS.cBa, Scer\GAL4^NP0001

(Nassari et al., 2022)

NOT suppressed by

Statement

Reference

Rab21^GD9402, Scer\GAL4^NP0001 has adult gut phenotype, non-suppressible by Rab11^{Q70L.UASp.YFP}, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has lipid droplet phenotype, non-suppressible by apolpp^HM05157, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has adult gut phenotype, non-suppressible by Tret1^HMS02573, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab21^GD9402, Scer\GAL4^NP0001 has adult gut phenotype, non-suppressible by apolpp^HM05157, Scer\GAL4^NP0001

(Nassari et al., 2022)

Suppressor of

Statement

Reference

Rab21^GD9402, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval plasmatocyte phenotype of Sbf^UAS.mCherry, Scer\GAL4^Cg.PA

(Jean et al., 2012)

Rab21^GD9402, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval plasmatocyte phenotype of Scer\GAL4^Cg.PA, mtm^UAS.mCherry

(Jean et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Sbf^GD12081 enhances the loss of F-actin rich protrusions seen in macrophages isolated from animals expressing Rab21^GD9042 under the control of Scer\GAL4^Cg.PA.

Expression of Rab21^GD9042 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing Sbf^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Cg.PA.

Expression of Rab21^GD9042 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing mtm^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Cg.PA.

(Jean et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rab21^GD9402 is rescued by Rab21^UAS.RR/Scer\GAL4^NP0001

(Nassari et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v32941

w¹¹¹⁸; P{GD9402}v32941

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab21^GD9042

Rab21^GD9402

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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