The endogenous Apc2 promoter drives expression of full-length, wild-type Apc2 which is tagged at the N-terminal end with a myristoylation sequence (Tag:Myr(Src64B)) and at the C-terminal end with EGFP.
The cuticle defects of embryos derived from Apc2g10 ApcQ8 males crossed to females containing double homozygous germline clones are strongly rescued by expression of Apc2T:Myr-Src64B,T:Avic\GFP-EGFP. The embryonic lethality is partially rescued (33% lethality is seen).
Apc2Tag:Myr(Src64B),EGFP partially rescues Apc2g10
Apc2T:Myr-Src64B,T:Avic\GFP-EGFP rescues the cuticle defects of embryos lacking both maternal and zygotic Apc2[+] function (homozygous Apc2g10 embryos derived from Apc2g10 females). The embryonic lethality of these Apc2 mutants is partially rescued (23% embryonic lethality is seen), and viable adults are obtained.