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General Information
Symbol
Dmel\bigmax1
Species
D. melanogaster
Name
FlyBase ID
FBal0284237
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
mlx1
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a 1232bp deletion which removes the entire bigmax coding region as well as 17 C-terminal amino acids of the neighbouring CG3368 gene.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

bigmax1/bigmax1 mutants display near complete pupal lethality.

Mutants die during the pharate adult stage and only a small number of adults are recovered. Mutants show a modest developmental delay when raised on standard fly food.

Mutant larvae fail to survive on a diet with 20% sucrose as the sole nutrient source. The mutant larvae develop with similar kinetics as controls on a high protein, low sugar diet, but as the sucrose concentration is increased, larval development is gradually slowed down, and at higher sucrose levels the larvae fail to pupate. The mutant larvae also show developmental delay in protein-rich food supplemented with either 10% glucose or 10% fructose, and are unable to pupate when raised on pieces of red grape with baker's yeast inoculum.

Mutant larvae show a significant increase in triglyceride species with long fatty acid tails and ceramide levels and a significant decreased in myristoleic acid and lauric acid levels compared to controls. They also show a decrease in total amino acid levels and a dramatic increase in the concentration of urea. Mutant larvae show an increase in hemolymph glucose compared to controls, showing a moderate increase when raised on a 20% yeast diet and a more prominent increase when raised on a 20% yeast, 5% sucrose diet. Mutant larvae show a prominent increase in hemolymph trehalose compared to controls when raised on a 20% yeast diet, with little further effect if they are raised on a 20% yeast, 5% sucrose diet. Total glycogen levels are significantly increased in mutant larvae raised on a 20% yeast, 5% sucrose diet.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

PepckΔ84/Df(2R)ED3636 rescues the lethality of bigmax1/bigmax1 mutants.

Expression of Aldh-IIIScer\UAS.cHa under the control of Scer\GAL4αTub84B.PL significantly improves the survival of bigmax1 animals on a 20% sucrose-only diet.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of bigmaxScer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4αTub84B.PL, Scer\GAL4ppl.PP or Scer\GAL4r4 rescues the sugar intolerance and pupal lethality of bigmax1 mutants.

Expression of bigmaxScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4r4, but not Scer\GAL4Mef2.PR, is sufficient to normalise circulating glucose levels in bigmax1 larvae.

Expression of bigmaxScer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4elav.PLu or Scer\GAL4Mef2.PR does not significantly improve the sugar tolerance or survival of bigmax1 mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)