FlyBase Allele Report: Dmel\trol[UAS.RG]

General Information

Symbol

Dmel\trol^UAS.RG

Species

D. melanogaster

Name

FlyBase ID

FBal0284928

Feature type

allele

Associated gene

Dmel\trol

Associated Insertion(s)

Carried in Construct

P{UAS-trol.RG}

Key Links

Transgenic product class

wild_type

(Cho et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Cho et al., 2012)

Mutagen

in vitro construct

(Cho et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-trol.RG}

Cytology

Description

UAS regulatory sequences drive expression of the trol-RG isoform.

(Cho et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

trol

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

trol^UAS.RG/Scer\GAL4^P52 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

trol^UAS.RG, Scer\GAL4^elav.PU is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of PlexA^UAS.Tag:HA, Scer\GAL4^elav.PU

(Cho et al., 2012)

Suppressor of

Statement

Reference

trol^UAS.RG/Scer\GAL4^ppl.PP is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of AdamTS-A^rnwy2/AdamTS-A^rnwy1

(Skeath et al., 2017)

Other

Statement

Reference

Scer\GAL4^tey-5053A, Sema1a^UAS.cYa, trol^UAS.RG has abnormal neuroanatomy | embryonic stage phenotype

(Cho et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

trol^UAS.RG/Scer\GAL4^P52 is an enhancer of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

trol^UAS.RG, Scer\GAL4^elav.PU is an enhancer of larval longitudinal connective | embryonic stage phenotype of PlexA^UAS.Tag:HA, Scer\GAL4^elav.PU

(Cho et al., 2012)

Suppressor of

Statement

Reference

trol^UAS.RG/Scer\GAL4^ppl.PP is a suppressor | partially of larval brain | third instar larval stage phenotype of AdamTS-A^rnwy2/AdamTS-A^rnwy1

(Skeath et al., 2017)

Other

Statement

Reference

Scer\GAL4^tey-5053A, Sema1a^UAS.cYa, trol^UAS.RG has larval RP5 motor neuron | embryonic stage phenotype

(Cho et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures such as imaginal discs) observed in AdamTS-A^rnwy1/AdamTS-A^rnwy2 mutant third instar larvae is partially suppressed by Scer\GAL4^ppl.PP-driven expression of trol^UAS.RG.

(Skeath et al., 2017)

Co-expression of trol^Scer\UAS.RG strongly enhances the commissural axon phenotype (failure to cross the midline) seen in embryos expressing Sema-1a^Scer\UAS.cYa under the control of Scer\GAL4^P52 in a Sema-1a^k13702 null background.

Co-expression of both trol^Scer\UAS.RG and Sema-1a^Scer\UAS.cYa under the control of Scer\GAL4^tey-5053A results in significant RP5 neuron defasciculation defects at the final choice point between muscles 12 and 13. Targeting defects are also seen, resulting in premature bifurcations.

Co-expression of trol^Scer\UAS.RG significantly enhances the aberrant midline crossing phenotype seen in longitudinal tracts of embryos expressing plexA^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PU.

(Cho et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

trol^UAS.RG/Scer\GAL4^elav.PU partially rescues trol^null

(Cho et al., 2012)

Fails to rescue

trol^UAS.RG/Scer\GAL4^twi.PU fails to rescue trol^null

(Cho et al., 2012)

Comments

Expression of trol^Scer\UAS.RG under the control of Scer\GAL4^elav.PU significantly rescues the ISNb and SNa motor axon guidance defects seen in trol^null embryos. Expression under the control of Scer\GAL4^repo.PU only very modestly rescues the motor axon defects, and expression under the control of Scer\GAL4^twi.PU fails to rescue the motor axon defects.

(Cho et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

65273

w^*; P{UAS-trol.RG}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

trol^Scer\UAS.RG

trol^UAS.RG

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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