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General Information
Symbol
Dmel\pblfortune
Species
D. melanogaster
Name
FlyBase ID
FBal0286138
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G7902139A

Reported nucleotide change:

G9321A

Comment:

G to A mutation in the splice donor site of the third intron of pbl.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Mutation at a splice donor site. This disrupts the splicing of the third intron, and the incompletely spliced transcript gains a premature stop codon, resulting in a short peptide being produced.

Nucleotide substitution: G9321A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Approximately 20% of homozygous stage 13 embryos show no left-right asymmetry in their hindgut, which normally curves to the right at this stage. A small fraction of the embryos showing inverse left-right asymmetry. The number of cells in the epithelium of the hindgut at stage 13 is significantly reduced compared to wild type. However, there is no significant correlation between the number of cells and the left-right asymmetry phenotype of homozygotes.

pbl2/pblfortune and pblfortune/Df(3L)BSC388 stage 13 embryos show defects in the left-right asymmetry in their hindgut, with some embryos show no asymmetry, and others showing inverse asymmetry.

Females carrying homozygous germline clones are sterile.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

pblScer\UAS.cPa reduces the frequency of defects in the left-right asymmetry of the hindgut by half in homozygous pblfortune embryos, even in the absence of a Scer\GAL4 driver. When expression is driven by Scer\GAL4arm.PS, Scer\GAL4byn-Gal4 or Scer\GAL4NP2432 the left-right asymmetry are rescued. Expression driven by either Scer\GAL4how-24B, Scer\GAL4elav.PU or Scer\GAL448Y does not increase level of rescue above that seen without a Scer\GAL4 driver.

Expression of pblScer\UAS.cPa under the control of Scer\GAL4byn-Gal4 rescues the reduced number of cells in the epithelium of stage 13 pblfortune homozygous embryos.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (1)