Late embryos from mothers both homozygous for tslΔ and expressing fogScer\UAS.P\T.cJa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 exhibit cuticle defects more similar to those of embryos from mothers only homozygous for tslΔ (i.e. some missing terminal structures and ventral-posterior cuticle hole) than those of embryos from mothers only expressing fogScer\UAS.P\T.cJa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 (i.e. only small amounts of recognizable cuticle remaining).
tslΔ torrv66 double mutants show a dramatically extended larval period compared to either mutant alone, with flies pupating 83 hours after controls. Double mutant adult flies are similar in size to tslΔ mutants alone.
A tslΔ mutant background does not suppress the advanced pupariation phenotype seen in flies expressing PtthScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4phm.PO. The decrease in male body size is further enhanced. The embryonic patterning defects seen when PtthScer\UAS.P\T.T:Ivir\HA1 is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 are also not suppressed.
The following defects exhibited by embryos from tslΔ mothers are partially rescued by one copy of tslT:Ivir\HA1 : the ventral cuticle hole, but not the "terminal class mutant" phenotype, in late embryos; both the irregularities/gaps in the ventral furrow and the exposure of mesodermal tissue (Twist-positive) at the dorsal surface, but not the elongated ventral furrow or the exposure of pole cells at the dorsal surface, in gastrulating embryos.
The expression of tslScer\UAS.T:Hsap\MYC,T:Avic\GFP-EGFP under the control of Scer\GAL4slbo.2.6 fully rescues the ventral cuticle hole and head defects, and partially rescues the patterning and cuticle defects exhibited by embryos from tslΔ mothers.
Expression of tslScer\UAS.cJa under the control of Scer\GAL4da-BG00643 rescues the delay in pupariation seen in tslΔ mutants. Expression of tslScer\UAS.cJa under the control of Scer\GAL4phm.PO is unable to rescue the phenotype.