FlyBase Allele Report: Dmel\α-Cat[1]

General Information

Symbol

Dmel\α-Cat¹

Species

D. melanogaster

Name

FlyBase ID

FBal0294012

Feature type

allele

Associated gene

Dmel\α-Cat

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Sarpal et al., 2012)

Mutagen

P-element activity

(Sarpal et al., 2012)

Progenitor genotype

P{EP}GE30561

(Sarpal et al., 2012)

Cytology

Description

Imprecise excision of P{EP}GE30561 removes a 2403bp region between the insertion site and the α-Cat. This deletes the first exon of α-Cat, including the translation start site and much of the intergenic region between α-Cat and the predicted gene CG32230.

(Sarpal et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:23,336,914..23,339,317 [-]

Comment:

A 2403bp deletion resulting from the imprecise excision of P{EP}GE30561 removes the first exon of alpha-Cat, including the translation start site, and much of the intergenic region between alpha-Cat and ND-MLRQ. (Reported R4 coordinates converted to R6)

(Sarpal et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adherens junction | adult stage | somatic clone

(Escobar et al., 2015)

apoptotic amnioserosa

(Jurado et al., 2016)

border follicle cell | adult stage, with α-Cat^{ST-D.UASp.Tag:HA}, Scer\GAL4^twi.PU

(Escobar et al., 2015)

border follicle cell | adult stage, with α-Cat^{ΔP.UASp.Tag:HA}, Scer\GAL4^twi.PU

(Escobar et al., 2015)

border follicle cell | somatic clone

(Desai et al., 2013, Sarpal et al., 2012)

cephalopharyngeal skeleton | embryonic stage

(Sarpal et al., 2012)

egg chamber | somatic clone

(Sarpal et al., 2012)

embryonic/larval cuticle | embryonic stage

(Jurado et al., 2016, Sarpal et al., 2012)

embryonic head

(Escobar et al., 2015, Desai et al., 2013)

follicle cell | adult stage | somatic clone

(Escobar et al., 2015)

follicle cell | somatic clone

(Desai et al., 2013)

imaginal disc | somatic clone | third instar larval stage

(Sarpal et al., 2012)

ovary | somatic clone

(Sarpal et al., 2012)

spectrin-associated cytoskeleton | adult stage | somatic clone

(Escobar et al., 2015)

Detailed Description

Statement

Reference

α-Cat¹ mutant embryos show anterior cuticle defects and frequently display holes, some also develop a completely dorsal open phenotype or dorsal holes. The mutant embryos have similar number of amnioserosa cells at the onset of dorsal closure but show an increase in the number of delamination events, this however has no effect on the velocity of progression of the leading edge during the dorsal closure.

(Jurado et al., 2016)

α-Cat¹/α-Cat¹ mutants die at a late embryonic stage with severe defects in head morphogenesis. α-Cat¹/α-Cat¹ follicle cell clones in the egg chamber display a loss of integrity of the follicle cell epithelium, with loss of cell contacts, adherens junctions and a collapse of the membrane-associated spectrin cytoskeleton.

Expression of α-Cat^{ΔP.Scer\UAS.P\T.T:Ivir\HA1} or α-Cat^{ST-D.Scer\UAS.P\T.T:Ivir\HA1} (but not α-Cat^{ST-A.Scer\UAS.P\T.T:Ivir\HA1}, α-Cat^{T645D.Scer\UAS.P\T.T:Ivir\HA1} or α-Cat^{T645A.Scer\UAS.P\T.T:Ivir\HA1}) under the control of Scer\GAL4^twi.PU in a α-Cat¹/α-Cat¹ mutant background results in a significant reduction in rate of border cell migration in the egg chamber, but migration is still completed during stage 10 of oogenesis, as compared to controls.

(Escobar et al., 2015)

α-Cat¹ mutant follicular epithelium cells lose cell-cell contacts and adopt a flattened, rounded shape, with loss of adherens junction markers. Border cells fail to migrate and remain at the anterior tip of the follicle.

(Desai et al., 2013)

The cuticle of α-Cat¹ mutant embryos shows severe defects in the head skeleton, which result from a failure in head involution. The failure of head involution leaves a large anterior opening in the epidermis through which internal organs are expelled, and the epidermis/cuticle shrinks as a result of muscle contractions. About 68% of embryos display strong head defects and 32% show weak head defects. The ventral and dorsal cuticle appear normal and no obvious defects are observed in other embryonic tissues.

Ultrastructural morphology of epithelial adherens junctions is normal in stage 16 α-Cat¹.

α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) do not develop in third instar larval imaginal discs.

α-Cat¹ mutant clones in the ovary (generated through recombination of the α-Cat^Ubi.PS rescue construct) display a variety of oogenesis defects. These include a compromised follicular epithelium leading to fused follicles and failure of border cell migration. The majority of α-Cat¹ mutant cells have lost contact with wild type follicle cells. These cells rarely form multilayered clusters, but are usually flattened and tightly attached to the basement membrane. α-Cat¹ mutant follicle cells appear to maintain apical-basal polarity, however neighbouring cells appear to lose polarity as their microvillus tufts point in different directions.

(Sarpal et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

α-Cat¹ has abnormal cell migration | somatic clone | adult stage phenotype, suppressible by α-Cat::shg^UASp.cSa/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, suppressible by α-Cat::shg^UASp.cSa/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has abnormal cell migration | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat::shg^ΔVH1.UASp

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat::shg^ΔVH1.UASp

(Desai et al., 2013)

α-Cat¹ has abnormal cell migration | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^Act.PU/α-Cat::shg^{VH3-CTD.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, suppressible by Scer\GAL4^Act.PU/α-Cat::shg^{VH3-CTD.UASp.Tag:HA}

(Desai et al., 2013)

α-Cat¹ has abnormal cell migration | somatic clone | adult stage phenotype, suppressible | partially by α-Cat::baz^UASp.Tag:HA/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has abnormal cell migration | somatic clone phenotype, suppressible | partially by Mmus\Ctnna2^Ubi.PD/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, suppressible by Mmus\Ctnna2^Ubi.PD/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, suppressible | partially by Scer\GAL4^Act.PU/Mmus\Ctnna2^Δ56.Ubi/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, suppressible | partially by α-Cat::shg^UASp.cSa/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Sarpal et al., 2012)

α-Cat¹ has FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone | third instar larval stage phenotype, suppressible by α-Cat::shg^UASp.cSa

(Sarpal et al., 2012)

NOT suppressed by

Statement

Reference

α-Cat¹ has abnormal cell migration | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/shg^UASp.cSa

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/shg^UASp.cSa

(Desai et al., 2013)

α-Cat¹ has abnormal cell migration | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat::baz^{bazOD.ΔVH1.UASp.Tag:HA}

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::baz^{bazOD.ΔVH1.UASp.Tag:HA}

(Desai et al., 2013)

α-Cat¹ has abnormal cell migration | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::baz^{ΔVH1.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::baz^{ΔVH1.UASp.Tag:HA}

(Desai et al., 2013)

α-Cat¹ has abnormal cell migration | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::baz^{αCatNbazODC.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::baz^{αCatNbazODC.UASp.Tag:HA}

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, non-suppressible by α-Cat::baz^UASp.Tag:HA/Scer\GAL4^Act.PU

(Desai et al., 2013)

α-Cat¹ has abnormal cell migration | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::ed^{ΔVH1.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::ed^{ΔVH1.UASp.Tag:HA}

(Desai et al., 2013)

α-Cat¹ has abnormal cell migration | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/Mmus\Ctnna1^Ubi.PD

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/Mmus\Ctnna1^Ubi.PD

(Desai et al., 2013)

α-Cat¹ has abnormal cell migration | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/Mmus\Ctnna2^Δ56.Ubi/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has lethal - all die during embryonic stage phenotype, non-suppressible by α-Cat^UASp.Tag:HA/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Sarpal et al., 2012)

Other

Statement

Reference

Phenotype Manifest In

Enhanced by

Statement

Reference

α-Cat¹ has embryonic/larval cuticle | embryonic stage phenotype, enhanceable by Df(1)Vinc²

(Jurado et al., 2016)

α-Cat¹ has embryonic/larval cuticle | embryonic stage phenotype, enhanceable by sqh^DD.UAS/Scer\GAL4^c381

(Jurado et al., 2016)

α-Cat¹ has embryonic/larval cuticle | embryonic stage phenotype, enhanceable by shg[+]/shg^g119

(Sarpal et al., 2012)

Suppressed by

Statement

Reference

α-Cat¹ has follicle cell | somatic clone phenotype, suppressible by α-Cat::shg^UASp.cSa/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, suppressible by α-Cat::shg^UASp.cSa/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, suppressible by α-Cat::shg^UASp.cSa/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has follicle cell | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat::shg^ΔVH1.UASp

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat::shg^ΔVH1.UASp

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat::shg^ΔVH1.UASp

(Desai et al., 2013)

α-Cat¹ has follicle cell | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act.PU/α-Cat::shg^{VH3-CTD.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act.PU/α-Cat::shg^{VH3-CTD.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, suppressible by Scer\GAL4^Act.PU/α-Cat::shg^{VH3-CTD.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, suppressible | partially by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat::baz^{bazOD.ΔVH1.UASp.Tag:HA}

(Desai et al., 2013)

α-Cat¹ has follicle cell | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act.PU/α-Cat::baz^{ΔVH1.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has follicle cell | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act.PU/α-Cat::baz^{αCatNbazODC.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, suppressible | partially by Scer\GAL4^Act.PU/α-Cat::baz^{αCatNbazODC.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has follicle cell | somatic clone phenotype, suppressible | partially by α-Cat::baz^UASp.Tag:HA/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, suppressible | partially by α-Cat::baz^UASp.Tag:HA/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has follicle cell | somatic clone phenotype, suppressible by Mmus\Ctnna2^Ubi.PD/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, suppressible | partially by Mmus\Ctnna2^Ubi.PD/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, suppressible by Mmus\Ctnna2^Ubi.PD/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has follicle cell | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/Mmus\Ctnna1^Ubi.PD

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, suppressible | partially by Scer\GAL4^Act.PU/Mmus\Ctnna2^Δ56.Ubi/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has embryonic/larval cuticle | embryonic stage phenotype, suppressible | partially by Arpc1[+]/Arpc1^Q25st

(Sarpal et al., 2012)

α-Cat¹ has embryonic/larval cuticle | embryonic stage phenotype, suppressible | partially by Arp3^EP3640/Arp3[+]

(Sarpal et al., 2012)

α-Cat¹ has embryonic/larval cuticle | embryonic stage phenotype, suppressible | partially by SCAR^Δ37/SCAR[+]

(Sarpal et al., 2012)

α-Cat¹ has embryonic/larval cuticle | embryonic stage phenotype, suppressible | partially by α-Cat::shg^UASp.cSa/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Sarpal et al., 2012)

α-Cat¹ has imaginal disc | somatic clone | third instar larval stage phenotype, suppressible by α-Cat::shg^UASp.cSa

(Sarpal et al., 2012)

α-Cat¹ has ovary | somatic clone phenotype, suppressible by α-Cat::shg^UASp.cSa

(Sarpal et al., 2012)

α-Cat¹ has egg chamber | somatic clone phenotype, suppressible by α-Cat::shg^UASp.cSa

(Sarpal et al., 2012)

α-Cat¹ has border follicle cell | somatic clone phenotype, suppressible by α-Cat::shg^UASp.cSa

(Sarpal et al., 2012)

NOT suppressed by

Statement

Reference

α-Cat¹ has follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/shg^UASp.cSa

(Desai et al., 2013)

α-Cat¹ has follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::ed^{ΔVH1.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::ed^{ΔVH1.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::ed^{ΔVH1.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/Mmus\Ctnna1^Ubi.PD

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/Mmus\Ctnna1^Ubi.PD

(Desai et al., 2013)

α-Cat¹ has follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/Mmus\Ctnna2^Δ56.Ubi/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/Mmus\Ctnna2^Δ56.Ubi/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/shg^UASp.cSa

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/shg^UASp.cSa

(Desai et al., 2013)

α-Cat¹ has follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat::baz^{bazOD.ΔVH1.UASp.Tag:HA}

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat::baz^{bazOD.ΔVH1.UASp.Tag:HA}

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::baz^{ΔVH1.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::baz^{ΔVH1.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has border follicle cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/α-Cat::baz^{αCatNbazODC.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ has embryonic head phenotype, non-suppressible by α-Cat::baz^UASp.Tag:HA/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

Other

Statement

Reference

α-Cat¹, shg[+]/shg^g119 has embryonic/larval cuticle | ventral | embryonic stage phenotype

(Sarpal et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the embryonic cuticle defects characteristic for α-Cat¹ mutants is further enhanced by combination with Df(1)Vinc² or by expression of sqh^DD.Scer\UAS under the control of Scer\GAL4^c381 in the mutant background.

(Jurado et al., 2016)

Expression of shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU does not suppress the phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also not rescued.

Ubiquitous expression of shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the embryonic head defects and lethality seen in α-Cat¹ mutant embryos.

Expression of α-Cat::shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Ubiquitous expression of α-Cat::shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. Animals are unable to develop beyond the larval stages.

Expression of α-Cat::shg^{ΔVH1.Scer\UAS.P\T} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Ubiquitous expression of α-Cat::shg^{ΔVH1.Scer\UAS.P\T} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. Animals are unable to develop beyond the larval stages.

Expression of α-Cat::shg^{ΔVH3-CTD.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also partially rescued.

Ubiquitous expression of α-Cat::shg^{ΔVH3-CTD.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. Animals are unable to develop beyond the larval stages.

Expression of α-Cat::baz^{bazOD.ΔVH1.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU does not suppress the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also not rescued.

Ubiquitous expression of α-Cat::baz^{bazOD.ΔVH1.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues embryonic head defects seen in α-Cat¹ mutant embryos. The lethality is not rescued.

Expression of α-Cat::baz^{ΔVH1.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are not rescued.

Ubiquitous expression of α-Cat::baz^{ΔVH1.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU does not rescue the head involution defects and lethality seen in α-Cat¹ mutant embryos.

Expression of α-Cat::baz^{αCatNbazODC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are not rescued.

Ubiquitous expression of α-Cat::baz^{αCatNbazODC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues the embryonic head defects seen in α-Cat¹ mutant embryos. The lethality is not rescued.

Expression of α-Cat::baz^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also partially rescued.

Ubiquitous expression of α-Cat::baz^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU does not rescue the embryonic head defects and lethality seen in α-Cat¹ mutant embryos.

Expression of α-Cat::ed^{ΔVH1.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU does not suppress the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are not rescued.

Ubiquitous expression of α-Cat::ed^{ΔVH1.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU does not rescue the embryonic head defects and lethality seen in α-Cat¹ mutant embryos.

(Desai et al., 2013)

One copy of shg^g119 causes a significant enhancement of the cuticle defects seen in α-Cat¹ mutant embryos. >99% of embryos display a strong head defect and 21% show defects in the ventral cuticle.

One copy of Arpc1^Q25st partially suppresses the cuticle defects seen in α-Cat¹ mutant embryos. The phenotype is more severe when Arpc1^Q25st is introduced maternally rather than paternally.

One copy of Arp3^EP3640 partially suppresses the cuticle defects seen in α-Cat¹ mutant embryos. Embryos show less severe defects on average and a fraction of embryos have normal heads.

One copy of SCAR^Δ37 partially suppresses the cuticle defects seen in α-Cat¹ mutant embryos. Embryos show less severe defects on average and a fraction of embryos have normal heads. The phenotype is more severe when SCAR^Δ37 is introduced maternally rather than paternally.

Expression of shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the embryonic lethality seen in α-Cat¹ mutants.

Expressing α-Cat::shg^{Scer\UAS.P\T.cSa} allows α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) to develop in third instar larval imaginal discs. The follicle epithelium and border cell migration defects are also rescued.

Expressing shg^{Scer\UAS.P\T.cSa} does not allow α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) to develop in third instar larval imaginal discs. Mutant clone cells generated in the follicle epithelium lose cell contacts and border cells fail to migrate.

Expression of α-Cat::shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues head morphogenesis and embryonic lethality in 89% of α-Cat¹ mutants, but animals die during the larval stages.

(Sarpal et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Ctnna2^{Scer\UAS.P\T.cDa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are partially rescued.

Ubiquitous expression of Mmus\Ctnna2^{Scer\UAS.P\T.cDa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the head involution defects seen in α-Cat¹ mutant embryos. Animals are unable to develop beyond the pupal stages.

Expression of Mmus\Ctnna1^{Scer\UAS.P\T.cDa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are not rescued.

Ubiquitous expression of Mmus\Ctnna1^{Scer\UAS.P\T.cDa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the head involution defects seen in α-Cat¹ mutant embryos. Animals are unable to live beyond the embryonic stages.

Expression of Mmus\Ctnna2^{Δ56.Scer\UAS.P\T} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to suppress the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are not rescued.

Ubiquitous expression of Mmus\Ctnna2^{Δ56.Scer\UAS.P\T} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues the head involution defects seen in α-Cat¹ mutant embryos. Some animals are able to develop to the larval stages.

(Desai et al., 2013)

Complementation and Rescue Data

Rescued by

α-Cat¹ is rescued by α-Cat^UASp.Tag:HA/Scer\GAL4^unspecified

(Escobar et al., 2015)

α-Cat¹ is rescued by α-Cat^UASp.Tag:HA/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ is rescued by Scer\GAL4^Act.PU/α-Cat^UASp.cDa/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ is rescued by α-Cat^UASp.Tag:HA/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Sarpal et al., 2012)

α-Cat¹ is rescued by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat^{ΔCTD.UAS.Tag:HA}

(Sarpal et al., 2012)

Partially rescued by

α-Cat¹ is partially rescued by Scer\GAL4^twi.PU/α-Cat^{ΔP.UASp.Tag:HA}

(Escobar et al., 2015)

α-Cat¹ is partially rescued by α-Cat^{T645A.UASp.Tag:HA}/Scer\GAL4^twi.PU

(Escobar et al., 2015)

α-Cat¹ is partially rescued by α-Cat^{T645D.UASp.Tag:HA}/Scer\GAL4^twi.PU

(Escobar et al., 2015)

α-Cat¹ is partially rescued by α-Cat^{ST-A.UASp.Tag:HA}/Scer\GAL4^twi.PU

(Escobar et al., 2015)

α-Cat¹ is partially rescued by α-Cat^{ST-D.UASp.Tag:HA}/Scer\GAL4^twi.PU

(Escobar et al., 2015)

α-Cat¹ is partially rescued by α-Cat^{UASp.ΔCTD.Tag:HA}/Scer\GAL4^Act.PU/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ is partially rescued by Scer\GAL4^Act.PU/α-Cat^{UASp.ΔVH2.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ is partially rescued by Scer\GAL4^Act.PU/α-Cat^{UASp.ΔVIN.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

α-Cat¹ is partially rescued by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat^{UASp.ΔVH2C.Tag:HA}

(Desai et al., 2013)

α-Cat¹ is partially rescued by Scer\GAL4^Act.PU/α-Cat^{Δ64.UASp.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

Not rescued by

α-Cat¹ is not rescued by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat^{UASp.ΔVH1.Tag:HA}

(Desai et al., 2013)

α-Cat¹ is not rescued by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat^{UASp.ΔVH3-CTD.Tag:HA}

(Desai et al., 2013)

α-Cat¹ is not rescued by Scer\GAL4^Act.PU/α-Cat^{UASp.ΔVH3.Tag:HA}/Scer\GAL4^da.G32

(Desai et al., 2013)

Comments

Expression of α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^unspecified rescues the embryonic head morphogenesis and lethality of α-Cat¹/α-Cat¹ mutants, and rescues the follicular epithelium integrity defects of α-Cat¹/α-Cat¹ follicle cell clones.

Expression of α-Cat^{ΔP.Scer\UAS.P\T.T:Ivir\HA1}, α-Cat^{T645A.Scer\UAS.P\T.T:Ivir\HA1}, α-Cat^{T645D.Scer\UAS.P\T.T:Ivir\HA1}, α-Cat^{ST-A.Scer\UAS.P\T.T:Ivir\HA1}, or α-Cat^{ST-D.Scer\UAS.P\T.T:Ivir\HA1}, under the control of Scer\GAL4^twi.PU rescues the embryonic head morphogenesis and partially rescues the lethality of α-Cat¹/α-Cat¹ mutants, and partially rescues the follicular epithelium integrity defects of α-Cat¹/α-Cat¹ follicle cell clones.

(Escobar et al., 2015)

Expression of α-Cat^{Scer\UAS.P\T.cDa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.cDa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects and lethality seen in α-Cat¹ mutant embryos.

Expression of α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects and lethality seen in α-Cat¹ mutant embryos.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH1.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). Border cell migration does not take place.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.ΔVH1.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the embryonic head defects and lethality seen in α-Cat¹ mutant embryos.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH3-CTD.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). Border cell migration does not take place.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.ΔVH3-CTD.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. The lethality is partially rescued.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH3.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). Border cell migration does not take place.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.ΔVH3.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the embryonic head defects and lethality seen in α-Cat¹ mutant embryos.

Expression of α-Cat^{Scer\UAS.P\T.ΔCTD.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fully rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). In most cases the border cell migration defects are also rescued.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.ΔCTD.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. The lethality is partially rescued.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH2.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are partially rescued.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.ΔVH2.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues the head involution defects that result in lethality in α-Cat¹ mutant embryos, but the animals are unable to develop beyond the larval stages.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH2N.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are partially rescued.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.ΔVH2N.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. No adults are seen but the animals are able to develop beyond the embryonic stages, with some animals reaching the pupal stage.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH2C.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fully rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are almost completely rescued.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.ΔVH2C.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. The lethality is partially rescued; some adults are recovered but many animals are unable to develop beyond the larval and pupal stages.

Expression of α-Cat^{Scer\UAS.P\T.ΔVIN.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU almost completely rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.ΔVIN.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. The lethality is partially rescued; adults are recovered but some animals are unable to develop beyond the larval and pupal stages.

Expression of α-Cat^{Δ64.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are partially rescued.

Ubiquitous expression of α-Cat^{Δ64.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the head involution defects seen in α-Cat¹ mutant embryos. The lethality is not rescued; the animals are unable to develop beyond the larval stages.

(Desai et al., 2013)

Expression of α-Cat^{ΔCTD.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the lethality seen in α-Cat¹ mutant embryos.

Expression of α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic lethality seen in α-Cat¹ mutants to fertile adults.

Expressing α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} allows α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) to develop in third instar larval imaginal discs. The follicle epithelium and border cell migration defects are also rescued.

(Sarpal et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (20)

Bloomington

65605

w¹¹¹⁸; α-Cat¹/TM3, P{GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb¹ Ser¹

65607

w¹¹¹⁸; P{tubP-GAL80}LL10, P{Ubi-α-Cat.S}4A P{neoFRT}40A; P{Act-GAL4.U}3, α-Cat¹/TM6B, Tb¹

65579

w¹¹¹⁸; P{tubP-GAL80}LL10, P{Ubi-α-Cat.S}4A P{neoFRT}40A; P{Act-GAL4.U}3, P{UASp-shg.S}attP2 α-Cat¹/TM6B, Tb¹