FB2021_02, released April 13, 2021

Allele: Scer\GAL4DBD.R93A09

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General Information
Symbol
Scer\GAL4DBD.R93A09
Species
S. cerevisiae
Name
FlyBase ID
FBal0304484
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{R93A09-GAL4.DBD}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - coding region fusion
(Aso et al., 2014)
in vitro construct - regulatory fusion
(Aso et al., 2014)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{R93A09-GAL4.DBD}
      Cytology
      Nature of the lesion
      Statement
      Reference

      The R93A09 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

      (Aso et al., 2014)
      Allele components
      Product class / Tool use(s)
      Encoded product / tool
      GAL4(DBD)::Zip-
      Associated Sequence Features
      GMR93A09
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      dopaminergic PAM neuron 10 | faint

      Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB047B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 11 | faint

      Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB047B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 5 | intense

      Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB047B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 6

      Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB047B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 8 | faint

      Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB047B')

      (Aso et al., 2014)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R93A09 and Hsap\RELAAD.R58E02 in adults results in a non-significant attraction to the illuminated area, no attraction is observed in controls.

      (Lewis et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Aso et al., 2014)

      FlyBase curator comment: According to correspondence with the authors, some split combinations listed in the methods of the paper are incorrect; the correct combinations (MB002B=R12C11-AD+R14C08-DBD, MB316B=R58E02-AD+R93G08-DBD, MB056B=R76F05-AD+R80G12-DBD, MB047B=R58E02-AD+R93A09-DBD) are listed on FlyLight so these are the ones that have been associated with the paper.

      (Lewis et al., 2015)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.R93A09.T:Zzzz\Zip-
      Scer\GAL4DBD.R93A09
      Name Synonyms
      Secondary FlyBase IDs
        References (6)