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General Information
Symbol
Hsap\RELAAD.R52H03
Species
H. sapiens
Name
FlyBase ID
FBal0304519
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The R52H03 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
MB-alpha neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{R14E06-GAL4.DBD} (combination referred to as 'MB502B')

when combined with P{ple-GAL4.DBD} (combination referred to as 'MB504B')

mushroom body medial-vertical lobe arborizing neuron 1

Comment: when combined with P{ple-GAL4.DBD} (combination referred to as 'MB504B')

mushroom body pedunculus-medial lobe arborizing neuron 1

Comment: when combined with P{R14E06-GAL4.DBD} (combination referred to as 'MB502B')

when combined with P{ple-GAL4.DBD} (combination referred to as 'MB504B')

mushroom body vertical lobe arborizing neuron 1

Comment: when combined with P{R14E06-GAL4.DBD} (combination referred to as 'MB502B')

when combined with P{ple-GAL4.DBD} (combination referred to as 'MB504B')

MB-alpha neuron of the dopaminergic PPL1 cluster | faint

Comment: when combined with P{ple-GAL4.DBD} (combination referred to as 'MB504B')

Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using shi1.UAS at 30-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.ple and Hsap\RELAAD.R52H03 in adults, only during aversive (CS+) odor re-exposure following training, does not prevent the aversive olfactory memory extinction that results from re-exposure, compared to controls.

Adult females prefer optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.ple and Hsap\RELAAD.R52H03 to darkness with no activation; this preference is significantly greater than that of controls for red light compared to darkness.

Inhibition (using shi1.UAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.ple and Hsap\RELAAD.R52H03 in adults significantly reduces aversive, but not appetitive, 1-min visual memory, compared to controls; inhibition does not significantly affect shock preference, compared to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.ple and Hsap\RELAAD.R52H03, in combination with a paired light stimulus, results in a significant increase in short-term associative aversive visual memory, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\RELAAD.R52H03.T:Zzzz\Zip+
Hsap\RELAAD.R52H03
Name Synonyms
Secondary FlyBase IDs
    References (12)