FB2021_01, released Feb 18, 2021

Allele: Hsap\RELAAD.R52H03

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General Information
Symbol
Hsap\RELAAD.R52H03
Species
H. sapiens
Name
FlyBase ID
FBal0304519
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{R52H03-p65.AD}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - coding region fusion
(Aso et al., 2014)
in vitro construct - regulatory fusion
(Aso et al., 2014)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{R52H03-p65.AD}
      Cytology
      Nature of the lesion
      Statement
      Reference

      The R52H03 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

      (Aso et al., 2014)
      Allele components
      Product class / Tool use(s)
      Encoded product / tool
      p65(AD)::Zip+
      Associated Sequence Features
      GMR52H03
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      MB-alpha neuron of the dopaminergic PPL1 cluster

      Comment: when combined with P{R14E06-GAL4.DBD} (combination referred to as 'MB502B')

      when combined with P{ple-GAL4.DBD} (combination referred to as 'MB504B')

      (Aso et al., 2014, Vogt et al., 2014)
      mushroom body medial-vertical lobe arborizing neuron 1

      Comment: when combined with P{ple-GAL4.DBD} (combination referred to as 'MB504B')

      (Aso et al., 2014, Vogt et al., 2014)
      mushroom body pedunculus-medial lobe arborizing neuron 1

      Comment: when combined with P{R14E06-GAL4.DBD} (combination referred to as 'MB502B')

      when combined with P{ple-GAL4.DBD} (combination referred to as 'MB504B')

      (Aso et al., 2014, Vogt et al., 2014)
      mushroom body vertical lobe arborizing neuron 1

      Comment: when combined with P{R14E06-GAL4.DBD} (combination referred to as 'MB502B')

      when combined with P{ple-GAL4.DBD} (combination referred to as 'MB504B')

      (Aso et al., 2014, Vogt et al., 2014)
      MB-alpha neuron of the dopaminergic PPL1 cluster | faint

      Comment: when combined with P{ple-GAL4.DBD} (combination referred to as 'MB504B')

      (Aso et al., 2014)
      dopaminergic PPL1 neuron | subset

      Comment: when combined with P{Ddc-GAL4.DBD}

      when combined with P{ple-GAL4.DBD}

      (Cichewicz et al., 2017)
      dopaminergic PPM3 neuron | subset

      Comment: when combined with P{Ddc-GAL4.DBD}

      when combined with P{ple-GAL4.DBD}

      (Cichewicz et al., 2017)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using shi1.UAS at 30-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.ple and Hsap\RELAAD.R52H03 in adults, only during aversive (CS+) odor re-exposure following training, does not prevent the aversive olfactory memory extinction that results from re-exposure, compared to controls.

      (Felsenberg et al., 2018)

      Adult females prefer optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.ple and Hsap\RELAAD.R52H03 to darkness with no activation; this preference is significantly greater than that of controls for red light compared to darkness.

      (Takemura et al., 2017)

      Inhibition (using shi1.UAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.ple and Hsap\RELAAD.R52H03 in adults significantly reduces aversive, but not appetitive, 1-min visual memory, compared to controls; inhibition does not significantly affect shock preference, compared to controls.

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.ple and Hsap\RELAAD.R52H03, in combination with a paired light stimulus, results in a significant increase in short-term associative aversive visual memory, compared to controls.

      (Vogt et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Aso et al., 2014)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.R52H03.T:Zzzz\Zip+
      Hsap\RELAAD.R52H03
      Name Synonyms
      Secondary FlyBase IDs
        References (12)