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General Information
Symbol
Dmel\pathdg50
Species
D. melanogaster
Name
dendrite growth defective 50
FlyBase ID
FBal0305310
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

A 172-nucleotide deletion in path that causes a frameshift and premature stop.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A 172-nucleotide deletion in path that causes a frameshift and premature stop, truncating the transmembrane domains. This should render the encoded protein nonfunctional.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

pathdg50/pathdg50 mutants do not show significant defects in larval growth. pathdg50/Df(3L)BSC773 larvae show defects in C4da dendrite growth, with pathdg50/Df(3L)BSC773 mutants showing significant reductions in dendrite length compared to wild type. C4da dendrite growth is more severely affected in PNS neurons located in more posterior abdominal segments (A2 vs A5 vs A8), with significantly shorter dendrites and fewer branches in pathdg50/Df(3L)BSC773 larvae compared to wild type. Axonal arbors show progressive growth defects that are more severe in posterior segments (similar to controls prior to 48hr AEL, and by 120hr AEL C4da neurons in posterior segments show severe axon growth defects), compared to wild type. pathGFP/pathdg50 or pathGFP/pathΔ larvae show normal dendrite growth in C4da neurons.

pathdg50 larvae show severe dendrite growth defects in the large C4da and medium C3da dendritic arbors but no obvious effect on the smaller C1da arbor (or chordotonal or bipolar dendrite neurons) or whole animal growth. Early growth and patterning of C4da neurons proceed normally, but, beginning in late first larval instar larvae, two growth defects are manifest. First, C4da dendrite length plateaus at ~3.5 mm, which the mutants reach by ~36-40 h after egg laying, whereas control C4da dendrites continue growing throughout larval development, reaching ~20 mm. Second, C4da dendrite arbors are remodeled after reaching their maximum length in the mutants: terminal dendrites are progressively lost, whereas primary dendrites continue to grow, maintaining a constant value of total dendrite length.

pathKG06640/pathdg50 or pathdg50/Df(3L)BSC773 larvae show severe dendrite growth defects in the large C4da dendritic arbors.

C4da dendrites in larvae generated from pathdg50 germline clones arrested at the same value of dendrite growth as zygotic pathdg50 mutants.

pathdg50 C4da MARCM clones exhibit dendrite growth defects.

Cell size in the epidermis, oenocytes, fat body or salivary glands of pathdg50/Df(3L)BSC773 larvae is not reduced - only the size of neurons with extreme growth requirements (C4da/C3da neurons) appear to be affected.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Phenotype Manifest In
NOT Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Dendrite growth in pathdg50/Df(3L)BSC773 animals arrests at the same value in the absence or presence of Scer\GAL4ppk.PG-mediated expression of Pi3K92ECAAX.Scer\UAS or RafScer\UAS.cSa.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Scer\GAL4Act5C.PI-mediated expression of pathA.Scer\UAS.T:Avic\GFP rescues the dendrite defects in pathdg50 mutants.

A heat-shock induced pulse of expression of pathA.Scer\UAS.T:Avic\GFP (using Scer\GAL4hs.PB) at 24, 48, 72 or 96 hours AEL is sufficient to significantly rescue dendrite growth defects of pathdg50/Df(3L)BSC773 animals.

pathA.Scer\UAS.T:Avic\GFP expression in pathdg50 MARCM clones rescues their C4da dendrite growth defects.

Scer\GAL4ppk.PG-mediated expression of pathA.Scer\UAS.T:Avic\GFP or pathA.Scer\UAS.T:Avic\GFP, but not pathΔN.Scer\UAS.T:Avic\GFP or pathΔC.A.Scer\UAS.T:Avic\GFP, rescues the dendrite growth defects of pathdg50/Df(3L)BSC773 animals.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
dendrite growth defective 50
Secondary FlyBase IDs
    References (4)