FlyBase Allele Report: Dmel\Sox21a[6]

General Information

Symbol

Dmel\Sox21a⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0318124

Feature type

allele

Associated gene

Dmel\Sox21a

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Zhai et al., 2015)

Mutagen

CRISPR/Cas9

(Zhai et al., 2015)

Progenitor genotype

Cytology

Description

A small deletion in the DNA-binding domain of Sox21a, the HMG domain, resulting in a frameshift and premature stop.

(Zhai et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:14,106,674..14,106,680 [-]

Comment:

7 bp deletion in the Sox21a DNA-binding domain, resulting in a frameshift and premature stop.

(Zhai et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intestinal cancer

CEA

(Zhai et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of intestinal cancer

is ameliorated by Mmp2^RNAi.UAS

(Zhai et al., 2015)

is ameliorated by Timp^UAS.cPa

(Zhai et al., 2015)

is ameliorated by bsk^DN.UAS

(Zhai et al., 2015)

is ameliorated by hep¹

(Zhai et al., 2015)

is exacerbated by puc^E69

(Zhai et al., 2015)

is ameliorated by upd2^HMS00901

(Zhai et al., 2015)

is ameliorated by upd2^NIG.5988R

(Zhai et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Zhai et al., 2015)

viable

(Zhai et al., 2015)

Phenotype Manifest In

adult anterior midgut epithelium

(Zhai et al., 2015)

enterocyte | somatic clone

(Zhai et al., 2015)

enteroendocrine cell | somatic clone

(Zhai et al., 2015)

Detailed Description

Statement

Reference

In contrast to wild type anterior midgut clones, which contain both enterocytes and enteroendocrine cells, Sox21a⁶ mutant clones along the whole midgut remain undifferentiated. Intestinal stem cell (ISC) division is reduced compared to controls, with a stronger effect in the posterior compared with the anterior midgut. There is a non-cell autonomous effect on ISC cell proliferation around the Sox21a⁶ mutant clone.

The accumulation of large clusters of progenitors in the anterior midgut (AMG) but not posterior midgut (PMG) is seen in Sox21a⁶ flies. These clusters contain both intestinal stem cells and enteroblasts, but progressively become dominated by enteroblasts. Similarly to the wild-type midgut, intestinal stem cells are localized basally, while enteroblasts are found more apically towards the lumen. These clusters increase in size over time and grow towards the intestinal lumen. The increase in intestinal stem cell number is nearly linear but the increase in enteroblasts is exponential. As in wild type, the enteroblasts do not undergo mitosis. Stimulating intestinal stem cell proliferation by infecting Sox21a⁶ mutant flies with bacteria (Ecc15) increases the size and numbers of tumors. Neighbouring enterocytes are eliminated, and this effect is not suppressed when apoptosis is inhibited in enterocytes. There is an increase in reactive oxygen species at the border of Sox21a⁶ mutant tumors compared with the tumor site itself. Sox21a⁶ mutant flies that have been fed the antioxidant, N-acetylcysteine amide (AD4) have a reduced tumor burden, although this reduction is not significantly significant.

(Zhai et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Sox21a⁶ has neoplasia phenotype, enhanceable by puc^E69/puc[+]

(Zhai et al., 2015)

Suppressed by

Statement

Reference

Sox21a⁶ has neoplasia phenotype, suppressible | partially by Egfr^DN.UAS.cBa/Scer\GAL4^NP5130/Scer\GAL80^ts.αTub84B

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by Scer\GAL4^NP0001/upd2^HMS00901

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by Scer\GAL4^NP0001/upd2^NIG.5988R

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by upd2^Δ/upd2^Δ

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/upd3^RNAi.UAS

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by upd3^Δ/upd3^Δ

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by spi^KK105786/Scer\GAL4^Su(H).GBE

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^Su(H).GBE

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^NP0001

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/Mmp2^RNAi.UAS

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/Mmp1^RNAi.UAS

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/kay^Fbz.UAS

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/Timp^UAS.cPa

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, suppressible | partially by hep[+]/hep¹

(Zhai et al., 2015)

NOT suppressed by

Statement

Reference

Sox21a⁶ has neoplasia phenotype, non-suppressible by upd1^GD1158/Scer\GAL4^Su(H).GBE

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, non-suppressible by Krn^KK107190/Scer\GAL4^NP0001

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, non-suppressible by Scer\GAL4^Su(H).GBE/wg^GD5007

(Zhai et al., 2015)

Sox21a⁶ has neoplasia phenotype, non-suppressible by Scer\GAL4^Su(H).GBE/wg^KK108857

(Zhai et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Sox21a⁶ has adult anterior midgut epithelium phenotype, enhanceable by puc^E69/puc[+]

(Zhai et al., 2015)

Suppressed by

Statement

Reference

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by Egfr^DN.UAS.cBa/Scer\GAL4^NP5130/Scer\GAL80^ts.αTub84B

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by Scer\GAL4^NP0001/upd2^HMS00901

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by Scer\GAL4^NP0001/upd2^NIG.5988R

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by upd2^Δ/upd2^Δ

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/upd3^RNAi.UAS

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by upd3^Δ/upd3^Δ

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by spi^KK105786/Scer\GAL4^Su(H).GBE

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^Su(H).GBE

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^NP0001

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/Mmp2^RNAi.UAS

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/Mmp1^RNAi.UAS

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/kay^Fbz.UAS

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/Timp^UAS.cPa

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, suppressible | partially by hep[+]/hep¹

(Zhai et al., 2015)

NOT suppressed by

Statement

Reference

Sox21a⁶ has adult anterior midgut epithelium phenotype, non-suppressible by upd1^GD1158/Scer\GAL4^Su(H).GBE

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, non-suppressible by Krn^KK107190/Scer\GAL4^NP0001

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, non-suppressible by Scer\GAL4^Su(H).GBE/wg^GD5007

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, non-suppressible by Scer\GAL4^Su(H).GBE/wg^KK108857

(Zhai et al., 2015)

Sox21a⁶ has adult anterior midgut epithelium phenotype, non-suppressible by spi^KK105786/Scer\GAL4^NP0001

(Zhai et al., 2015)

Suppressor of

Statement

Reference

Sox21a⁶ is a suppressor of adult posterior midgut epithelium phenotype of Scer\GAL4^NP0001, upd2^UAS.cUa

(Zhai et al., 2015)

NOT Suppressor of

Statement

Reference

Sox21a⁶ is a non-suppressor of adult anterior midgut epithelium phenotype of Scer\GAL4^NP0001, upd2^UAS.cUa

(Zhai et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

A Sox21a⁶ mutant background suppresses the increase in intestinal stem cells (ISCs) usually seen in the posterior midgut when upd2^Scer\UAS.cUa is expressed in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001}. An increase in ISCs is only seen in the anterior midgut.

Expression of Egfr^{DN.Scer\UAS.cBa} in the midgut cell progenitors under the control of Scer\GAL4^esg-NP5130 (limited to the adult stages using Scer\GAL80^ts.αTub84B) suppresses the formation of tumors in the anterior midgut of Sox21a⁶ mutant flies.

Expression of upd2^HMS00901 in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} strongly reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Expression of upd2^NIG.5988R in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} strongly reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Homozygous upd2^Δ reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Expression of upd1^GD1158 in enteroblasts under the control of Scer\GAL4^Su(H).GBE does not reduce tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Expression of Krn^KK107190 in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} does not reduce tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Expression of wg^GD5007 in enteroblasts under the control of Scer\GAL4^Su(H).GBE does not reduce tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Expression of wg^KK108857 in enteroblasts under the control of Scer\GAL4^Su(H).GBE does not reduce tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Expression of upd3^{dsRNA.Scer\UAS} in enteroblasts under the control of Scer\GAL4^Su(H).GBE reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Homozygous upd3^Δ reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Expression of spi^KK105786 in enteroblasts under the control of Scer\GAL4^Su(H).GBE reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies. The reduction is not significant when spi^KK105786 is expressed in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001}.

Expression of bsk^DN.Scer\UAS in enteroblasts under the control of Scer\GAL4^Su(H).GBE reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies. Expression of bsk^DN.Scer\UAS in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} also suppresses tumor formation and the presence of delaminating enterocytes.

Expression of Mmp2^{dsRNA.Scer\UAS} in enteroblasts under the control of Scer\GAL4^Su(H).GBE reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Expression of Mmp1^{dsRNA.Scer\UAS} in enteroblasts under the control of Scer\GAL4^Su(H).GBE does not reduce tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Expression of kay^Fbz.Scer\UAS in enteroblasts under the control of Scer\GAL4^Su(H).GBE reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

Expression of Timp^Scer\UAS.cPa in enteroblasts under the control of Scer\GAL4^Su(H).GBE reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

One copy of hep¹ reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

One copy of puc^E69 increases tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

(Zhai et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sox21a⁶ is rescued by Sox21a^UAS.Enh/Scer\GAL4^Tub.PU

(Zhai et al., 2015)

Sox21a⁶ is rescued by Sox21a^{UAS.Enh.mRFP1}

(Zhai et al., 2015)

Comments

Expression of Sox21a^Scer\UAS.Enh under the control of Scer\GAL4^tub.PU suppresses the differentiation and intestinal stem cell division defects seen in Sox21a⁶ mutant adult midgut clones.

Expression of Sox21a^{Scer\UAS.Enh.T:Disc\RFP} reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

(Zhai et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

68389

w^*; sna^Sco/CyO; Sox21a⁶ P{FRT(w^hs)}2A

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Sox21a⁶

(Chen and St Johnston, 2022, Zhai et al., 2017, Zhai et al., 2015)

Sox21a^SK6

(Zhai et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close